摘要
AIM:Toinvestigatetheabilityofproteaseinhibitorstomodulatehistaminereleasefromhumancolonmastcells.METHODS:Enzymaticallydispersedcellsfromhumancolonwerechallengedwithanti-IgEorcalciumionophoreA23187intheabsenceorpresenceoftryptaseandchymaseinhibitors,andhistaminereleasewasdetermined.RESULTS:IgEdependenthistaminereleasefromcolonmastceilswasinhibitedbyuptoapproximately37%,26%and36.8%bychymaseinhibitorsZ-Ile-Glu-Pro-Phe-CO2Me(ZIGPFM),N-TosyI-L-phenylalanyl-chloromethylketone(TPCK),andC~l-antitrypsin,respectively.Similarly,inhibitorsoftryptaseleupeptin,N-tosyI-L-lysinechloromethylketone(TLCK),lactoferrinandprotaminewerealsoabletoinhibitanti-IgEinducedhistaminereleasebyamaximumofsome48%,37%,40%and34%,respectively.Preincubationoftheseinhibitorswithcellsfor20rainbeforechallengedwithanti-IgEhadsmalleffectontheinhibitoryactionsoftheseinhibitorsoncolonmastcells.Aspecificinhibitorofaminopeptidaseamastatinhadnoeffectonanti-IgEinducedhistaminerelease.Thesignificantinhibitionofcalciumionophoreinducedhistaminereleasewasalsoobservedwiththeinhibitorsoftryptaseandchymaseexamined.Apartfromleupeptinandprotamine,theinhibitorstestedbythemselvesdidnotstimulatecolonmastcells.CONCLUSION:ItwasdemonstratedthatbothtryptaseandchymaseinhibitorscouldinhibitIgEdependentandcalciumionophoreinducedhistaminereleasefromdispersedcolonmastcellsinaconcentrationdependentofmanner,whichsuggestthattheyarelikelytobedevelopedasanovelclassofanti-inflammatorydrugstotreatchronicofcolitisinman.
出版日期
2004年03月13日（中国期刊网平台首次上网日期，不代表论文的发表时间）
