Detection of Trichomonas Vaginalis Infection in Male Non-gonococcal Urethritis Patients by InPouch TV Culture and Polymerase Chain Reaction-中国期刊网

摘要 Objective:TostudytheprevalenceofTrichomonasvaginalis(TV)infectioninChinesemalepatientswithnongonococcalurethritis(NGU),toevaluatethesensitivityandspecificityofurine-basedandurethralswabpolymerasechainreaction(PCR)detection,tosetupamethodfornon-invasivedetectionofmaleTVinfection.Method:OnehundredandfivemaleNGUpatientswereselectedfromaBeijingSTDclinic.Twourethralswabswereobtainedfromeachpatient,onefortheInPouchTVculturesystemandtheotherforPCR.Inaddition,onefirstvoidurinespecimenwascollectedforPCRdetection.Culturewasconsideredthe“goldstandard”.Thesensitivity,specificity,positivepredictivevalue(PPV)andnegativepredictivevalue(NPV)ofthetwoPCRdetectionswerecomparedtocultureresults.Results:Theprevalenceofurine-basedPCRandurethralswabPCRdetectionwas3.81%(4/105)and4.76%(5/105)respectively.Comparedtoculture,thesensitivity,specificity,PPVandNPVwere80%,100%,100%and99%forurine-basedPCRand80%,99%,80%and99%forurethralswabPCR.Conclusion:TVisoneoftheetiologicalagentsinmaleNGU,witha4.76%prevalenceofinfectioninourstudy.TheurinebasedPCRdetectionhashighersensitivityandspecificityandprovidesanoninvasivemethodmorefeasibleinpractice.

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Detection of Trichomonas Vaginalis Infection in Male Non-gonococcal Urethritis Patients by InPouch TV Culture and Polymerase Chain Reaction

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Detection of Trichomonas Vaginalis Infection in Male Non-gonococcal Urethritis Patients by InPouch TV Culture and Polymerase Chain Reaction

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