ISOLATION　OF　HUMAN　TRANSCRIPTS　EXPRESSED　IN　16HBE　CELLS　RELATED　TO　CHLOROPHYLLIN　ANTITRANSFORMING　ACTIVITY　AGAINST　ANTI-BPDE　BY　cDNA　REPRESENTATIONAL　DIFFERENCE　ANALYSIS-中国期刊网

摘要 Objective:ToanalyzethedifferentiallyexpressedcDNAsequencesrelatedtochlorophyllin(CHL)mediatedinhibitionofmalignanttransformationofhumanbronchia1epithelialcellline(16HBE).Methods:16HBEcellstreatedwithchlorophyllinandanti-BPDEwereconductedastester,16HBEcellstreatedonlywithanti-BPDEwereconductedasdriver,andcDNArepresentationaldifferenceanalysis(cDNARDA)wasusedtocomparethedifferentialgeneexpressionbetweenthetwokindsofcells.ThecDNAfragmentswereligatedtopGEM-TvectorandtransformedintoJM109bacteria.TheplasmidDNAwassequencedandcomparedwithdatabaseinGenBankbyBLASTN.Results:Amongthe5clonedcDNAsequences,threewerenovelandwereregisteredindbESTdatabase,twoshowedsequencehomologytoalpha-enolaseandanewlyfoundgeneribosomalproteinS18/S6-like.Conclusion:These5cDNAsequencesmightplayimportantrolesinantitransformingeffectofchlorophyllin.

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ISOLATION　OF　HUMAN　TRANSCRIPTS　EXPRESSED　IN　16HBE　CELLS　RELATED　TO　CHLOROPHYLLIN　ANTITRANSFORMING　ACTIVITY　AGAINST　ANTI-BPDE　BY　cDNA　REPRESENTATIONAL　DIFFERENCE　ANALYSIS

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ISOLATION OF HUMAN TRANSCRIPTS EXPRESSED IN 16HBE CELLS RELATED TO CHLOROPHYLLIN ANTITRANSFORMING ACTIVITY AGAINST ANTI-BPDE BY cDNA REPRESENTATIONAL DIFFERENCE ANALYSIS

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ISOLATION　OF　HUMAN　TRANSCRIPTS　EXPRESSED　IN　16HBE　CELLS　RELATED　TO　CHLOROPHYLLIN　ANTITRANSFORMING　ACTIVITY　AGAINST　ANTI-BPDE　BY　cDNA　REPRESENTATIONAL　DIFFERENCE　ANALYSIS

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