摘要
Theirradiationofcellscombinedwiththeimmunoconjugateofgoldnanoparticlesbytheshortpulselasercanmaketheplasmamembranebetransientlypermeabilized,whichcanbeusedtotransferexogenousmoleculesintothecells.Weexplorethistechniqueasanovelgenetransfectionmethodforfloatingcells.Threedifferentfloatingcellsexposedtothelaserareselectivelytransfectedwithfiuoresceinisothiocyanatedextran,antibody,andgreenfluorescentprotein(GFP)codingplasmids,andtheviabilityofcellsaredeterminedbypropidiumiodide.Forfluoresceinisothiocyanate-dextran,thebesttransfectionefficiencyof65%isobtained;fortheantibody,itis74%;whereasforthegreenfluorescentproteincodingplasmids,averysmalltransfectionefficiencyisgained.Ifthetransfectionefficiencyisimproved,goldnanoparticleswillbeveryusefulasmediatorforgenetransfectioninlivingcells.
出版日期
2009年10月20日（中国期刊网平台首次上网日期，不代表论文的发表时间）
