简介：Changesofsoilorganicnitrogenformsandsoilnitrogensupplyundercontinuousricecroppingsystemwereinvestigatedinalong-termfertilizationexperimentinJinhua,ZhejiangProvince,China.ThefertilizertreatmentsincludedcombinationofP-K,N-K,N-P,andN-P-Kaswellasthecontrol.Aftersixyearsofcontinuousdouble-ricecropping,totalsoilNandhydrolysableNcontentsremainedstableinplotswithNtreatments,whilethehydrolysableNcontentsweresubstantiallyreducedinthoseplotswithoutNapplication.Comparedtotheunbalancedfertilizationtreatments,PandKincreasedthepercentageofhydrolysableammoniumNinthetotalsoilNwiththebalancedapplicationofN,andalsomaintainedhigherricegrainyieldsandnitrogenuptake.GrainyieldwaspositivelycorrelatedwithtotalNuptake(r=0.875**),hydrolysableN(r=0.608**),hydrolysableammoniumN(r=0.560**)andthehydrolysableunknownN(r=0.417**).TotalNuptakewaspositivelycorrelatedwithhydrolysableN(r=0.608**),hydrolysableammoniumN(r=0.440**)andhydrolysableunknownN(r=0.431**).Soilnutrientdepletionand/orunbalancedfertilizationtoricecropreducedNcontentinsoilmicrobialbiomass,andthereforeincreasedC/Nratio,suggestinganegativeeffectonthetotalmicrobialbiomassinthesoil.