简介：　　目的：分析和总结体外循环心脏手术后合并真菌感染的临床特点及耐药情况。方法：对51例体外循环心脏直视术后继发真菌感染的临床资料进行统计分析,分析术后培养分离出的78株感染真菌的药敏试验结果。结果：心脏术后长期使用广谱抗生素及皮质激素、机械通气、气管切开、APACHEⅡ高评分、全肠外营养及侵入性检查治疗等均是真菌感染高危因素。心脏术后真菌感染部位以下呼吸道为主,其次为血液、静脉导管顶端,继之为泌尿系。大多数真菌菌株对两性霉素B、5-氟胞嘧啶敏感,对大扶康及伊曲康唑部分耐药。结论：合理使用广谱抗生素和糖皮质激素,尽量减少不必要的侵入性操作,加强全身营养及脏器功能支持,改善肠道微生态平衡,密切监测真菌感染的一切临床迹象,注重预防及经验性治疗;治疗上应选择敏感、毒副作用轻的抗真菌药物。

简介：由于近年急性心肌梗死(AMI)患者存活率大大提高,发生急性心肌再梗死(简称再梗)者明显增加.本文分析51例再梗者心电图变化,报告如下.

简介：C-反应蛋白是机体非特异性炎症反应的敏感标志物,可能参与心血管疾病的发生和发展,当它增高时,可能预测未来心血管事件的发生.对C-反应蛋白在某些方面也存在激烈的争论.

简介：BackgroundTheprognosticvalueofserumC-reactiveprotein(CRP)inpatientswithinfectiveendocarditis(IE)isnotwellelucidated.ThisstudyaimedtoevaluatetheusefulnessofCRPinpredictingtheoutcomeofIE.MethodsTwohundredninty-sixpatientsfrom2009to2012intheDepartmentofCardiologyatGuangdongGeneralHospitalwerescreenedanddividedintosurgicalandconventionaltreatmentgroups.CRP,whitebloodcell(WBC),erythrocytesedimentationrate(ESR)andotherclinicaldatawereobtainedwithfollow-upfor12months.ResultsTwohundredthirty-sixpatientswereassignedtoreceivesurgerytreatmentwhile60patientsreceivedconventionaltreatment.Inthesurgerygroup,thelevelofCRPinthedeathpatientswassignificantlyhigherthanthatinthesurvivalpatients(P<0.001).TheareaunderthecurveofROCwasabout0.749(SE0.064,P=0.005,95%CI,0.624-0.874)andthecut-offpointofCRPwas23.8mg/L.Inconventionalgroup,therewassignificantdifferencebetweendeathandsurvival(P<0.001).TheareaunderthecurveofROCwasabout0.701(SE0.095,P=0.032,95%CI,0.515-0.888)andthecut-offpointsofCRPwas65.6mg/L.TherewerenosignificantdifferencesinWBCandESRbetweensurgeryandconventionalgroups.ConclusionAmoreaggressivesurgicalinterventionresultsinabetteroutcomeoverconventionaltreatmentandCRPcouldbeservedasapredictivemarkerforadverseoutcomeinIEpatients.

简介：研究疾病:晚期心力衰竭.目的:比较血管紧张素转换酶抑制和直接血管扩张对晚期心衰预后的影响.设计:随机式.病人资料:117例充血性心力衰竭者,NYHAⅢ级或Ⅳ级,休息时有血液动力学异常,为心脏移植做评估.

简介：番茄是人们餐桌上常有的蔬果,有人说要生吃,有人说要熟吃,到底番茄怎么吃才更有益健康呢？我们购买番茄时应避免选择未成熟的青色番茄,因其含有毒的龙葵碱,吃多了可能导致中毒,出现头晕、恶心、呕吐及全身疲乏等症状,严重的还有生命危险。因此宜选择个大、圆润、丰满、已经熟透的番茄食用。

简介：目的评估低密度脂蛋白胆固醇（LDL—C）／高密度脂蛋白胆固醇（HDL—C）比值对经皮冠脉介入（PcI）术后患者心血管事件的预测价值。方法选择急性冠脉综合征（ACS）并予前降支置入支架的患者119例，依据血浆LDL—C／HDL—C比值将患者分为3组，随访1年，评估三组患者心血管事件发生率，以及各危险因素与心血管事件发生率的关系。结果①与LDL—C／HDL—C比值较低的两组相比，比值较高组患者体重指数、女性患者百分率、吸烟人数及糖化血红蛋白、高敏C反应蛋（hs—CRP）、总胆固醇和LDL—C水平均明显升高，而HDL—C水平和他汀类药物使用率则较低（P〈0．05）。②第1组风险（HR）1．04，95％可信区间（c，）0．98-1．08，第2组HR1．16，95％C11．08-1．20，第3组HR1．27，95％C11．19～1．36（P〈0．05）。随着LDL—C／HDL—C比值的升高，PCI术后1年患者心血管事件发生率也逐渐升高（P〈0．05）。③Cox比例风险回归模型提示，LDL—C／HDL—C比值对PCI术后心血管事件风险的预测价值优于其他危险因素。结论LDL—C／HDL—C比值对PCI术后患者1年内心血管事件再发具有一定的预测价值。

简介：根据一项新的研究，添加服用维生素C或E不能帮助预防年龄相关性白内障。白内障或眼睛的晶状体浑浊是老年人失明的主要原因。年龄相关性白内障影响一半以上超过65岁的美国人。手术去除白内障是唯一有效的治疗方法，并且除了戒烟和多吃富含水果与蔬菜的饮食之外，关于如何预防它们知道的很少。

简介：一个新的研究显示,血液C反应蛋白(C-reactiveprotein,简称CRP)水平高并不会使人发生心脏病和中风的风险升高。C反应蛋白是一种反应人体炎症状态的蛋白。近年来C反应蛋白很受关注,因为有证据显示它与心脏病相关,可能是心脏病的标志物之一。但疑问仍然存在,到底C反应蛋白只是反映心脏存在问题,还是它本身可以导致心脏疾病?

简介：ObjectivesTostudythedepressiveeffectoftheantisenseoligonuceotides(ASODN)ofc-mycandproliferatingcellnuclearantigen(PCNA)ontheproliferationofVSMC.MethodsTakingtheVSMCobtainedfromrataortathoracaliscultured4～8generationasresearchobject.Theobjectsweredividedintothreegroupstocarryoutcontrolstudy:controlgroup,PCNAASODNgroupandc-mycASODNgroup.TheASODNs'workingconcentrationallwere1:50.ThedepressiveeffectofASODNonVSMCproliferationwasinvestigatedbycellcounting,MTTand3H-TdRincorporationassay;PCNAandc-mycexpressionweredetectedbyimmunohistochemicalmethodaftertransferringPCNAandc-mycASODNintoVSMC.ResultsPCNAandc-mycASODNcouldinhibittheproliferationofVSMCsignificantly,comparedwithcontrolgroup(P<0.05).②TransferringPCNAandc-mycASODNintoVSMCobtainedsuccessfully;thecorrespondinggenewasinhibitedobviously;comparedwithcontrolgroup(P<0.05).ConclusionsPCNAandc-mycmightplayaconsiderableroleintheVSMCproliferationprocess.ThecorrespondinggenecouldbedepressedsuccessfullyaftertransferringPCNAandc-mycASODNintoVSMC,andthentheproliferationofVSMCwassloweddown.Thisstudypresentedabeneficialproposalandtheoreticalfundamentforatherosclerotictreatment.

简介：Bothendotheliallipasegene(LIPG)584C>T(rs2000813)polymorphismandalcoholconsumptionmodulateserumlipidlevels.Buttheirinteractionsonserumlipidprofilesarenotwellknown.ThepresentstudywasundertakentodetecttheinteractionsofLIPG584C>Tpolymorphismandalcoholconsumptiononserumlipidlevels.GenotypingoftheLIPG584C>Twasperformedin763nondrinkersand520drinkersaged15-85.Interactionsbetweenthegenotypesandalcoholconsumptionwereassessedbyusingacross-productterm.Thelevelsofserumtotalcholesterol(TC),triglyceride(TG),high-densitylipoproteincholesterol(HDL-C),apolipoprotein(Apo)AI,andtheratioofApoAItoApoBwerehigherindrinkersthaninnondrinkers(P<0.01forall).Therewasnosignificantdifferenceinthegenotypicandallelicfrequenciesbetweennondrinkersanddrinkers.ThelevelsofserumTC,HDL-CandApoAIinnondrinkersweredifferentamongthethreegenotypes(P<.05-.01).ThesubjectswithCTgenotypehadhigherserumTC,HDL-CandApoAIlevelsthanthesubjectswithCCgenotype.ThelevelsofserumHDL-CandApoAIindrinkersweredifferentamongthethreegenotypes(P<.001andP<.05;respectively).TheindividualswithTTgenotypehadhigherserumHDL-CandApoAIlevelsthantheindividualswithCCandCTgenotypes.ThelevelsofTCinnondrinkerswerecorrelatedwithLIPG584C>Tallele(P<.05),whereasthelevelsofTGandHDL-CwereassociatedwithLIPG584C>Talleles(P<.05)andgenotypes(P<.05);respectively.ThepresentstudysuggeststhatthesubjectswithTTgenotypebenefitedmorefromalcoholconsumptionthanthesubjectswithCTandTTgenotypesinincreasingserumHDL-CandApoAIlevels.

简介：backgroundBonemarrowmesenchymalstemcells(BMSCs)canbeisolatedandculturedtomanypassages.However,StemcellsincludingBMSCsquicklyundergosenescenceinculture.Thecellsenescenceandmulti-directionaldifferentiationhavehamperedproducingBMSCsinquantitywiththeirundifferentiatedstate.Inthisstudywereportanaturalcompound,vitaminC(Vc),maintainsBMSCsstemproperty.MethodsHumanBMSCswereisolatedfrombonemarrowandpurifiedby1.073g/mLdensitygradientcentrifugation.50ng/mLVcwereaddedtoBMSCsfordifferenttimepoint.FlowcytometrywasusedtodetectcellsurfacemarkersofBMSCswithorwithoutVctreatment.BMSCsproliferationwasanalyzedbyMTTassay.PCR(polymerasechainreaction)andreal-timePCRwereusedfordetectingc-kit,nanog,andOct-4genesexpressionlevels.DNAmethyltransferase(Dnmt)1andDnmt3blevelswerealsodetectedbyreal-timePCR.ResultsFlowcytometryshowedthatafterVctreatmentfor6h,thesurfacemarkersofBMSCswerealmostunchanged.VcincreasedtheproliferationactivityofBMSCsfrom6hto24h.PCRshowedtheexpressionofc-kit,nanog,andoct-4geneswereobviouslyincreasedinVctreatedgroupthancontrolgroupat12h.Real-timePCRshowedthatthelevelofc-kit,nanog,andoct-4geneswereunregulatedfrom6hto12hcomparedwithcontrolgroup.VcalsoincreasedDnmt3bbutnotDnmt1geneexpression.ConclusionsOurresultsshowedVcactsatleastacceleratesBMSCsproliferationandmaintainsstemcellproperty.Inourstudy,wehighlightedamethodofimprovingthespeedofBMSCsgenerationandprovidedadditionalinsightsintothemechanisticbasisofpreventingBMSCssenescence.

简介：BackgroundInthisstudy,weaimedtoevaluatetheimpactofabnormalglucose,lipidandCystatin-ConthevirtualPvectorcharacteristics,whichhaven’tbeenreportedinpreviousstudies.Methods204ofnon-diabetesmellitus(NDM),130ofDM(type2)and39ofimpairedglucosetolerance(IGT)patientswereconsecutivelyandretrospectivelyrecruited.Weselectedaone-minutelengthofelectrocardiogramat4AMforanalysis.Afteraseriesofcalculatingalgorisms,weobtainedthevirtualplanarPvectorparameters.ResultsTherewerenosignificantdifferencesinFPV,FPA,RSPV,RSPA,HPVandHPAgroups.Afteradjustingconfoundingfactors,theregressioncoefficients(RC)wereestimatedasfollow:forFPV,femalegender(RC-0.21,P=0.02),triglyceride(RC-0.09,P<0.01),RVOT(RC0.03,P=0.02);forRSPV,femalegender(RC-0.21,P<0.01),triglyceride(RC-0.10,P<0.01),averageheartrate(RC0.01,P=0.02);forHPV,triglyceride(RC-0.08,P<0.001),LDL(RC-0.19,P<0.01),ApoB(RC0.67,P<0.01);forRSPA,Btypeofblood(RC-22.06,P=0.02),Cystatin-C(RC-72.79,P=0.02),thicknessofinterventricularseptum(RC3.70,P=0.01).Cystatin-CwassuggestedasacurerelatedtoRSPA,andthecut-offpointwas1.6mg/L.TherewerenosignificantriskfactorsassociatedwithFPAandHPA.TherewasnodifferenceinvirtualPvectoramongDM,IGTandNDMgroups.ConclusionIncreasedlevelsoflipidandCystatin-CsignificantlyimpactthecharacteristicsofvirtualPvector,whereasglucosedoesnot.Thesechangesmaycomefromahigherlowvoltageatrialareaandabnormalorientationofatrialdepolarization.

简介：近年来的研究证实，动脉粥样硬化的发生发展与炎症密切相关。作为机体非特异性的炎症敏感指标C反应蛋白（CRP）与脑血管疾病的相关性日益备受关注。我们对我院住院诊治的急性脑卒中患者早期CRP检测结果进行了系统分析，进一步明确CRP与脑血管疾病的关系，现报道如下。

简介：目的探讨络风宁1号方对心肌梗死大鼠血栓调节素-活化蛋白C-内皮细胞蛋白C受体系统的调节作用。方法将SD大鼠随机分为假手术组（7只）、模型组（8只）、络风宁1号方组（7只）、联合用药组（8只）、卡托普利组（8只），心肌梗死造模后络风宁1号组及联合用药组予含生药量4.81g/（kg·d）的络风宁1号方汤剂，卡托普利组及联合用药组予卡托普利片7.5mg/（kg·d），假手术组及模型组予相同体积蒸馏水，2/d，连续4周。4周后测定各组大鼠心肌梗死边缘组织血栓调节蛋白（TM）、活化蛋白C（APC）、内皮细胞蛋白C受体（EPCR）基因及蛋白的表达。结果模型组大鼠EPCR、TM的mRNA、sEPCR、sTM含量较假手术组明显升高（P＜0.05），EPCR、TM的蛋白表达及APC含量较假手术组下降（P＜0.05）；络风宁1号组、联合用药组EPCR、TM的mRNA以及含量较模型组降低，差异有统计学意义（P＜0.05），EPCR蛋白表达较模型组增加，差异有统计学意义（P＜0.05）；络风宁1号组、联合用药组血清APC含量较模型组增加（P＜0.05）。结论络风宁1号方能够减轻膜结合型EPCR、TM的损伤，抑制了TM、EPCR的mRNA代偿性增加，使sEPCR、sTM含量下降，APC含量增加。

简介：BackgroundApolipoprotein(apo)A-Visanovelmemberoftheapolipoproteinclusterinvolvedintriacylglycerol(TG)homeostasis.IthasreportedthatAPOA5genepolymorphismsiscorrelatedwitharterioscleroticdiseases.However,ThisassociationisunknownonChinesepatientswithatheroscleroticcerebralinfarction.ThepresentstudyaimedtoelucidatetherelationshipofAPOA5-1131T>Candarterioscleroticcerebralinfarction(ACI)aswellasthelevelsofserumlipids.MethodsPolymerasechainreaction-restrictionfragmentslengthpolymorphisms(PCR-RFLP)analysis,enzymaticandimmunoturbidimetrymethodswereusedtomeasure-1131T>Cgenotype,allelefrequencyaswellasplasmalipidlevelof90ACIpatientsand221healthysubjectsofHanChinese.ResultsInACIgroup,thelevelofTGinalleleCcarrierswashigherthanthatofnon-Ccarriers(P<0.05).ThefrequencyofalleleCinACIgroupwashigherthaninhealthygroup(χ~2=5.568,P=0.018).Exceptsex,ageandBMI,thelevelsoftotalcholesterol(TC),triglycerides(TG),highdensitylipoproteincholesterol(HDL-C),low-densitylipoproteincholesterol(LDL-C),APOA1andAPOBinACIgroupdistinctivelywerehigherthanthoseinhealthygroup.ConclusionTheAPOA5-1131alleleCisassociatedwiththehighlevelofTGinACIpatients,whichisprobablylinkedwithACIdangerofChineseHan.

简介：目的；探讨高敏C反应蛋白（hsCRP）与高血压之间的关系。方法；选择56例高血压1，2，3级患者和52例正常对照者．测定并比较其血清hsCRP浓度。结果：高血压病患者血清hscRP浓度明显高于对照组（P〈0-05），高血压3级患者的血清hsCRP浓度显著高于高血压1，2级患者（P〈0.05）。结论：高血压病患者血清hscRP浓度显著增加，且hsCRP浓度可反映其血压水平，炎症反应可能参与了高血压病的发生。

简介：目的探讨急性脑卒中时C反应蛋白（CRP）的动态改变特点与临床生理指标、血生化指标、凝血指标之间的关系，了解急性脑卒中对CRP的影响。方法选择住院急性脑卒中患者55例（观察组），进行有关临床指标检查和不同时刻CRP的测定。另选在门诊的非脑血管病患者30例（对照组），测定CRP作为对照。结果观察组患者CRP动态变化显著高于对照组（P=0．0269～0．0006），发病初期CRP的变化与WBC计数、血糖、胆固醇变化显著相关，随后的增高则与年龄、甘油三酯、凝血功能、纤维蛋白原水平等有关，与格拉斯哥意识评分和巴塞尔神经功能评分呈负相关（P〈0．05，P〈0．01）。结论CRP的动态变化反映了机体损伤的实际状况，可以作为判断急性脑卒中严重程度和预后的有效指标．但这种变化会受到血液中多种因素的影响。

简介：目的:评价C-反应蛋白(CRP)对于急性心肌梗塞(AMI)患者预后价值的意义.方法:测定65例AMI患者的血清CRP含量,并观察其心脏事件的发生率.结果:CRP≥8mg/L的AMI患者其心力衰竭(17.24%),心律失常(20.69%),心脏事件发生率(40.83%)明显高于CRP＜8mg/L的13.89%,5.56%,5.56%(P＜0.01).结论:血C反应蛋白水平升高是急性心肌梗塞预后的预测因子.

简介：研究表明，炎性机制参与了动脉粥样硬化的病理过程。其中，炎性反应标记物C反应蛋白（CRP）的血清水平对缺血性脑卒中（ischemicstroke，IS）的发病、严重程度及预后具有重要临床价值。近年来，单核苷酸多态性（singlenucleotidepotymorphisms，SNP）遗传标记的出现，