简介：Thisexperimentusedwhole-cellpatch-clamptechniquetoinvestigatethecourseofrecoveryfromuse-dependentblockofNa+channels(Nav1.5)inhumanembryonickidney(HEK)cells,onwhichtoverifytheeffectsofvolatileoilofNardostachychinesisBatal(Gansong).MethodsTwopulsesgeneratedbycomputerfollowedbyarecoverypulseandatestpulse,theintervaldurationbetweenthetwopulsesvariedfrom16msto1s,andholdingpotentialis-80mVto-140mV.ThepeakNa+currentforagivenrecoverytimewasnormalizedtothefullyrecoveredpeakcurrent,andthenormalizedvaluewastheplotasafunctionoftherecoverytimetostudytheeffectsof3ppmconcentrationGansongvolatileoilonrecoveryfromuse-dependentblockofNav1.5inHEK.ResultsItshowedthatGansonggroup,comparingwithcontrolgroup,delayedthetimecoursesofrecoveryfromuse-dependentblock[(33.2±5.77)msforcontrolgroupand(52.5±6.08)msfor3ppmGansonggroup,P<0.05].InthepresenceofGansong,inhibitionoftheNa+currentwasenhancedbyincreasingfrequencyofdepolarizingpulsefrom56.5msto16ms.Inthecontrolgroup,thetimecourseofrecoveryshowedthatrecoverystartedat19.5msandfinishedby36.5ms.InthepresenceofGansong,thetimecourseofrecoveryshowedthatrecoverystartedat36.5msandfinishedby56.5ms.Na+currentsrecoveredfromtheuse-dependentblockvaryingwithholdingpotential(holdingpotential-dependent).ConclusionsTheresultssuggestedthatNa+currentsrecoveredfromtheuse-dependentblockcorrelatedwithpersistenttime,holdingpotential.TheGansongvolatileoilhasinhibitiveeffectontheNa+currentrecovery.