简介：AIM:Todeterminewhetherischemicpreconditioning(IP)affectsthedevelopmentofedematouscerulein-inducedpancreatitisandtoassesstheroleofcyclooxygenase-1(COX-1),COX-2,andheatshockprotein70(HSP70)inthisprocess.METHODS:InmaleWistarrats,IPwasperformedbydampingofceliacartery(twicefor5minat5-minintervals).ThirtyminutesafterIPorshamoperation,acutepancreatitiswasinducedbycerulein.ActivityofCOX-1orCOX-2wasinhibitedbyresveratrolorrofecoxib,respectively(10mg/kg).RESULTS:IPsignificantlyreducedpancreaticdamageincerulein-inducedpancreatitisasdemonstratedbytheimprovementofpancreashistology,reductioninserumlipaseandpoly-Cribonucleaseactivity,andserumconcentrationofpro-inflammatoryinterleukin(IL)-1β.Also,IPattenuatedthepancreatitis-evokedfallinpancreaticbloodflowandpancreaticDNAsynthesis.Serumlevelofanti-inflammatoryIL-10wasnotaffectedbyIP.Cerulein-inducedpancreatitisandIPincreasedthecontentofHSP70inthepancreas.MaximalincreaseinHSP70wasobservedwhenIPwascombinedwithcerulein-inducedpancreatitis.InhibitionofCOXs,especiallyCOX-2,reducedtheprotectiveeffectofIPinedematouspancreatitis.CONCLUSION:OurresultsindicatethatIPreducespancreaticdamageincerulein-inducedpancreatitisandthiseffect,atleastinpart,dependsontheactivityofCOXsandpancreaticproductionofHSP70.

简介：目的探讨热休克蛋白70（HSP70）、p53和增殖细胞核抗原（PCNA）在肝细胞癌（HCC）组织中的表达及其意义。方法采用免疫组化法检测正常人、慢性乙型肝炎、肝硬化和HCC肝或癌组织HSP70、p53和PCNA的表达情况。结果HCC组织HSP70、p53和PCNA表达阳性率明显高于非癌组织（x1^2=27.16x2^2=67.6,x3^2=40.6,P〈0.01）;HSP70在正常人、慢性乙型肝炎、肝硬化和HCC中的表达逐步增强;HSP70和p53在分化较好的HCC中的阳性率明显低于分化不良者（x1^2=6.8,P1〈0.01x2^2=6.1,P2〈0.05）,而PCNA表达与HCC组织分化程度无关（x2=2.4,P〉0.05）;HSP70表达强度与p53和PCNA表达关系密切（x1^2=41.3,x2^2=41.4,P〈0.01）。结论HCC是HSP70高表达肿瘤。HSP70表达与p53和PCNA表达密切相关,因而在HCC发生和发展中起重要作用。

简介：丙型肝炎病毒感染者糖代谢异常的发病率有不同程度的升高。除了肝源性糖尿病共有的发病机制外，胰岛β细胞功能的减低和胰岛素抵抗（IR）在丙型肝炎病毒感染者糖代谢异常的发病机制中起着重要作用。但IR可能更为重要，而肝脂肪变性、肿瘤坏死因子-α过表达、脂联素水平下降、细胞因子信号抑制因子合成以及铁代谢异常等都可能参与了IR的形成。

简介：AIM:Heatshockprotein(HSP)70isover-expressedinhumangastriccancerandplaysanimportantroleintheprogressionofthiscancer.WeinvestigatedtheeffectsofantisenseHSP70oligomeronhumangastriccancercelllineSGC-7901,anditspotentialroleingenetherapyforthiscancer.METHODS:HumangastriccancercelllineSGC-7901wastreatedinvitrowithvariousconcentrationsofantisenseHSP70oligonucleotidesatdifferentintervals.Growthinhibitionwasdeterminedaspercentagebytrypanbluedyeexclusiontest.ExtractedDNAwaselectrophoresedonagarosegel,anddistributionofcellcycleandkineticsofapoptosisinductionwereanalyzedbypropidiumiodideDNAincorporationusingflowcytometry,whichwasalsousedtodetecttheeffectsofantisenseoligomerpretreatmentonthesubsequentapoptosisinducedbyheatshockinSGC-7901cells.ProteinswereextractedforsimultaneousmeasurementofHSP70expressionlevelbySDS-PAGEWesternblotting.RESULTS:Thenumberofviablecellsdecreasedinadoseandtime-dependentmanner,andladder-likepatternsofDNAfragmentswereobservedinSGC-7901cellstreatedwithantisenseHSP70oligomersataconcentrationof10μmol/Lfor48hor8μmol/Lfor72h,whichwereconsistentwithinter-nucleosomalDNAfragmentation.Flowcytometricanalysisshowedadose-andtime-dependentincreaseinapoptoticratebyHSP70antisenseoligomers.ThisresponsewasaccompaniedwithadecreaseinthepercentageofcellsintheG1andSphasesofthecellcycle,suggestinginhibitionofcellproliferation.Inaddition,flowcytometryalsoshowedthatpretreatmentofSGC-7901cellswithHSP70antisenseoligomersenhancedthesubsequentapoptosisinducedbyheatshocktreatment.WesternblottingdemonstratedthatHSP70antisenseoligomersinhibitedHSP70expression,whichprecededapoptosis,andHSP70wasundetectableattheconcentrationof10μmol/Lfor48hor8μmol/Lfor72h.CONCLUSION:AntisenseHSP70oligomerscanabrogateHSP70expressioninSGC-7901cells,wh

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简介：功能性消化不良又称非溃疡性消化不良，是指一组无器质性原因的慢性或间歇性的上消化道症状。本病是近20年来随着人们对疾病认识观念的改变而提出的。临床表现主要是胃肠道症状，伴有失眠、焦虑、抑郁、头昏、头痛等功能性症状。本文采用多潘立酮加低聚木糖治疗功能性消化不良病人45例，取得了满意的效果，现报告如下。

简介：【摘要】目的：探讨在糖尿病患者的临床诊断中，血液生化检验的应用价值。方法：选取患有糖尿病的患者作为研究组进行研究，一共是60名，在对同一时间进行体检后身体健康的60名人员设置成健参照组进行对比研究，两组人员均接受血液生化检验，对两组人员的空腹血糖（FBG）、餐后2小时血糖（2hPBG）、葡萄糖耐量、糖化血红蛋白（HbA1c）、果糖胺（FMN）、总胆固醇（TC）及甘油三酯（TG）等指标进行了对比分析，还对研究组各生化指标的疾病检出率进行了分析。结果：研究组的FBG、2hPBG均显著高于参照组；研究组的HbA1c、FMN、TG、TC、葡萄糖耐量等指标也均高于参照组；在研究组别中，HbA1c、FMN、TG三个指标的检出率都要比葡萄糖耐量高，差异显著（P<0.05）。结论：采取血液生化检验这种方法可以更加准确的将糖尿病病人诊断出来，从而为患者的后续治疗提供可靠依据。