简介：<正>自1980年以来经临床及病理检查证实为上颌窦血管瘤者19例,为进一步提高诊治水平,将我们的经验报告如下。

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简介：<正>细菌性眼内炎是眼内的严重感染,对视力及眼球危害大,儿童眼内炎在眼内炎病人中占有较大比重。笔者复习了1994年元月1日至1997年5月31日的31例确诊为眼内炎的病历,其中19例为12岁以下儿童,且有其特点,分析如下。

简介：AIMTo调查反脉管的上皮的生长的效果在normoxic和hypoxic条件下面的纤维变性相关的煽动性的调停人的表示上的因素(VEGF)代理人，并且进一步澄清在anti-VEGFtherapy.METHODSHuman以后位于纤维变性下面的机制网膜的颜料上皮(RPE)细胞在normoxic和hypoxic条件下面被孵化。为组织缺氧处理，在200楲敭瑮??杧獥整?桴?敮慧楴敶挠牯敲慬楴湯戠瑥敷湥琠敨攠灸敲獳潩?景洠剩木?愠摮?楥?的CoCl2?乏?单佉呎敨攠灸敲獳潩?敬敶獬漠?業???洠剩木?愠摮洠剩??搠晩敦?敢睴敥?桴?散瑮慲?灥瑩敨楬浵漠?牴湡灳牡湥?湩慦瑮氠湥?湡?潣杮湥瑩污挠瑡牡捡?猠杵敧瑳湩?桴楥?湩潶癬浥湥?湩琠敨瀠瑡潨敧敮楳?景挠湯敧楮慴?慣慴慲瑣

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简介：AIM:ToinvestigatetheroleofRho-associatedproteinkinase(ROCK)inhibitor,Y27632,inmediatingtheproductionofextracellularmatrix(ECM)componentsincludingfibronectin,matrixmetallo-proteinase-2(MMP-2)andtypeIcollagenasinducedbyconnectivetissuegrowthfactor(CTGF)ortransforminggrowthfactor-β(TGF-β)inahumanretinalpigmentepithelialcellline,ARPE-19.METHODS:TheeffectofY27632ontheCTGForTGF-βinducedphenotypeinARPE-19cellswasmeasuredwithimmunocytochemistryasthechangeinF-actin.ARPE-19cellsweretreatedwithCTGF(1,10,100ng/mL)andTGF-β(10ng/mL)inserumfreemedia,andanalyzedforfibronectin,laminin,andMMP-2andtypeIcollagenbyRT-qPCRandimmunocytochemistry.CellswerealsopretreatedwithanROCKinhibitor,Y27632,toanalyzethesignalingcontributingtoECMproduction.·RESULTS:TreatmentofARPE-19cellsinculturewithTGF-βorCTGFinducedanECMchangefromacobblestonemorphologytoamoreelongatedswirlpatternindicatingamesenchymalphenotype.RT-qPCRanalysisanddifferentgeneexpressionanalysisdemonstratedanupregulationinexpressionofgenesassociatedwithcytoskeletalstructureandmotility.CTGForTGF-βsignificantlyincreasedexpressionoffibronectinmRNA(P=0.006,P=0.003respectively),lamininmRNA(P=0.006,P=0.005),MMP-2mRNA(P=0.006,P=0.001),COL1A1mRNA(P=0.001,P=0.001),COL1A2mRNA(P=0.001,P=0.001).PreincubationofARPE-19withY27632(10mmol/L)significantlypreventedCTGForTGF-βinducedfibronectin(P=0.005,P=0.003respectively),MMP-2(P=0.003,P=0.002),COL1A1(P=0.006,P=0.003),andCOL1A2(P=0.006,P=0.004)geneexpression,butnotlaminin(P=0.375,P=0.516).CONCLUSION:OurstudydemonstratedthatbothTGF-βandCTGFupregulatetheexpressionofECMcomponentsincludingfibronectin,laminin,MMP-2andtypeIcollagenbyactivatingtheRhoA/ROCKsignalingpathway.Duringthisprocess,ARPE-19cellswereshowntochangefromanepithelialtoamesenchymalphenotypeinvi