简介：PlantWRKYtranscriptionfactors(TFs)constituteoneofthelargestfamiliesofproteinsinvolvedinbioticandabioticstressresponses.TheseTFshaveaconserved60aminoacidWRKYdomainattheN-terminalandazincfingermotifattheC-terminal.ToexaminetherelevanceofOsWRKY72inimpartingsalinitystresstolerance,twoindicaricegenotypes,Rasi(tolerantgenotype)andTellahamsa(susceptiblegenotype),wereused.InRasiseedlingsat12hunder100mmol/LNaClstress,OsWRKY72expressionwasup-regulated,whereasinTellahamsa,itwashighlyup-regulatedatlethalstress.Full-lengthOsWRKY72cDNAwasclonedfromthesetworicegenotypesforfurtheranalysis.Weidentifiedavariant,termedasOsWRKY72bthatcarriesanadditionalsequenceof111bpwithintheWRKYdomain.ExpressionofOsWRKY72bwashigherundersalinitystressinRasithaninTellahamsa.DisorderpredictionofOsWRKY72bshowedthattheadditionalsequenceintheWRKYdomainisorderedtherebymaintainingthetertiarystructurethatmightinteractwiththemajorgrooveofDNA.PredictionofphosphorylationsitesinOsWRKY72bindicatedthatafewserineresiduescouldbethepotentialphosphorylationsites.Inthisstudy,wefirstlyreportedaOsWRKY72variantthatcouldhavearoleinabioticstressresponses.