简介：

简介：包括16不育系的米饭isonuclear-alloplasmic线和他们的维护者线的四个集合被使用91份SSR教材学习原子染色体和他们的相对关系的基因差异分析。169等位基因的一个总数在16根线被检测，与53.85%的多态的loci和每1.8的地点的等位基因的一个平均数字的频率，并且平均基因差异是0.228。无菌的线分享了的isonuclear-alloplasmicmale的四个集合146相同等位基因，相应于86.39%全部的等位基因；同时，在测试材料之中有23不同等位基因，是13.61%全部的等位基因。平均，78.70%相同等位基因和全部的等位基因的21.30%不同等位基因在isonuclear-alloplasmic不育系和他们的维护者线之间被检测。Therewere53.85%相同等位基因和在homozygousallonucleus不育系之中的全部的等位基因的46.15%不同等位基因。指纹为某不育系和维护者线被建立。测试的所有材料基于簇分析在0.2遗传距离被划分成三个组。HuanongA和HuayuA(包括的Huanong乐队HuayuB)的八根线在第一个组，KezhenA的四根线(包括KezhenB)在在第三个组的Zhenshan97A(包括的Zhenshan97B)的第二个组，和四根线。为theisonuclear-alloplasmic不育系，在Y(Yegong)类型和WA之间的类似系数(野未成功)打字或在CW(Raoping野米饭)和WA之间打到达87-98%。

简介：Threeresidualheterozygouslines(RHLs)carryingheterozygoussegmentsintheintervalsRM587-RM225,RM204-RM6119andRM6119-RM402ontheshortarmofricechromosome6,respectively,wereselectedfromaricepopulationderivedfromanRHLfortheintervalRM587-RM402.Tenmaternalhomozygotes,10paternalhomozygotesand20heterozygoteswereselectedfromeachoftheF2populationsderivedfromthethreeRHLs.Thethreesetsofnearisogeniclines(NILs)weregrowntodetectthegrainyieldperplant,numberofpaniclesperplant,numberoffilledgrainsperpanicleand1000-grainweight.WithanalysisonthephenotypicdifferencesamongthethreegenotypegroupsineachNILsetandthoseamongoverlappingchromosomesegmentsubstitutionlines,threeQTLsfornumberoffilledgrainsperpanicleandtwoQTLsforgrainyieldperplantwereresolved.Theywerelocatedintheintervalsrangingfrom0.66Mbto2.49Mb.Theadditiveeffectwashigherthanthedominanceeffectateachlocus.ThealleleforincreasingthetraitvaluewasderivedfromthepaternalparentatqNFGP6-1,andfromthematernalallelesatotherQTLs.Basedonthepresentstudy,anapproachforconstructingnewgeneticresourcetofacilitatefinemappingofQTLsinricewasproposed.

简介：TheDNAfragmentsabout1600bpwereamplifiedusingrandomamplifiedpolymorphismDNA(RAPD)primerOPA12withthetemplatesofmitochondrialDNAofZhenshan97AandZhenshan97B,andweresequenced.ThenucleotidesequencesandlengthsofthefragmentsfromZhenshan97AandZhenshan97Bshowednodifference.Thepreciselengthofthefragmentwas1588bp.Sequencecharacterizedamplificationregion(SCAR)primerswerethendevelopedtodiscriminatethecytoplasmicmalesterile(CMS)linesandtheirmaintainerlines.Aspecific1588bpfragmentcouldbeamplifiedwithSCARprimers,CHI19F2/CHI19R2andCHI20F3/CHI23R3,inthemitochondrialDNAofZhenshan97A,butnotZhenshan97B.Furthermore,thespecificfragmentcouldbealsoamplifiedfromthetotalDNAfromgreenleaftissuesofZhenshan97AwithSCARprimers,butnotZhenshan97B.Withthecorrespondingprimers,thespecificfragmentcouldalsobeamplifiedfromthetotalDNAofgreenleavesofothertwoCMSlineswithwildabortivetypecytoplasm(CMS-WA),namelyZhenpinAandTianfengA,butnotintheirmaintainerlines.Moreover,usingtotalDNAastemplate,eachofthefourpairsofSCARprimerscouldalsobeusedtoamplifythe1588bpfragmentinCMS-ID(Indonesiapaddytype)lineⅡ-32A,butnotinII-32B,andthespecificfragmentwasamplifiedfromtheDNAofbothF1andF2seedlingsofShanyou63.Theresultsofdetectingthegeneticpurityofaman-mademixtureoftheseedsofZhenshan97AusingCHI20F3/CHI23R3werecompletelyconsistentwiththephenotypes.Takentogether,theseresultsindicatedthatthespecific1588bp-fragmentamplifiedbyCHI20F3/CHI23R3wastheuniqueamplificationproductsofCMSmitochondrialDNA,andcouldbeusedtodistinguishCMS-WAandCMS-IDlinesfromtheircorrespondingmaintainerlinesattheseedlingstage.

简介：Genotypingbysequencing(GBS)istherecentapproachofnext-generationsequencingtechniquefordiscoveringandgenotypingsinglenucleotidepolymorphisms(SNPs)incropspecies.Genotypicvariationstudies(SNPsandinsertion-deletions/InDels)wereperformedusingfourricelinesbasedonGBSdatabyaligningtothereferencegenomeNipponbare.LocalaromaticricelandraceTulaipanjiwascrossedwithRanjit,andtwodistinctlineswereidentifiedfromtheprogenies:onelinewithawnsandaromatraitsandtheotherwithoutawnsandaroma.TotalnumberofSNPsandInDelsidentifiedwere52810and4327atreaddepth10,respectively.OutofthetotalpolymorphicSNPs/InDels,16490wereintergeneric,7812wereinsidegene,and4435wereintronic.Phylogenetically,Tulaipanjiwasclosertothereferencegenomenipponbare.Basedonrecurrentparentgenomeanalysis,outof10013alleles,92.52%wasintrogressedintoprogeny-awnfromTulaipanjiand7.48%fromRanjit,whereasprogeny-awnlesscarried89.19%allelesfromRanjitandonly10.81%allelesfromTulaipanji.Inaddition,progeny-awnwasthehighestheterozygous(83.88%)andprogeny-awnlesswastheleast(2.24%)atthisfifthgenerationofrecombinantinbredlines.TheseSNPvariationsmaybelinkedtothephenotypictraitsandcanbeutilizedincropimprovementthroughlinkagemapping.TheseresultssuggestthataddingahighdensityofSNPmarkerstoamappingorbreedingpopulationthroughGBShasagreatvaluefornumerousapplicationsinricebreedingandgeneticsresearch.

简介：Fieldperformanceofwhitebackedplanthopper(WBPH)-resistanceoffourphenotypeswasevaluatedinChunjiang06(CJ-06)/TN1DHricelines,whichwereexpressedbydifferentcombinationsofsuckinginhibitoryandovicidaltraitsinheritedindependentlyfromCJ-06.WBPHestablishedthehighestpopulationsinsusceptibleDHlinesthathadneithersuckinginhibitory-norovicidalresistance.Bothimmigrationandsubsequentpopulationlevelswerekeptbelowthedamage-causingdensityinthesuckinginhibitoryDHlinesevenunderaWBPHoutbreak.WBPHcouldnotbuilduppopulationsintheDHlineshavingboththesuckinginhibitoryandovicidalresistance.AlthoughWBPHimmigratedpreferentiallytonon-suckinginhibitoryDHlineswithovicidalresistance,subsequentpopulationbuildupwassignificantlysuppressed.ItwasconcludedthatthedifferentialperformancetoWBPH-resistanceinCJ-06/TN1DHlineswasprimarilyduetothesuckinginhibitorytrait,andcomplementarilytotheovicidaltrait.

简介：微卫星标记484/485和484/W2R被用来在米饭细菌原生质在Wx地点识别复等位基因。十五等位基因被使用微卫星班和G-T多型性在278accessions识别。在这些等位基因之中，(CT)_(12)-G,(CT)_(15)-G,(CT)_(16)-G,(CT)_(17)-G,(CT)_(18)-G并且(CT)_(21)-G没被报导。在Wx地点带不同等位基因的72根单个片断的替换线(SSSL)被使用Huajingxian74与开发(CT)是的_(11)-G等位基因作为施主在Wx地点包含12不同等位基因的一个接受者和20就职。代替的片断的估计的长度与17.4厘米的一般水准从2.2～77.3厘米。

简介：TounderstandthewildOryzagenomeeffectonphotosynthesisanditsrelationtototaldrymatteraccumulationinanelitericevariety,asetof40stableintrogressionlines(ILs)BC3F8derivedfromacrossofOryzasativa(KMR3)×Oryzarufipogon(WR120)weregrownunderwellwateredconditions.Leafgasexchangemeasurementsandleafchlorophyllestimateswereconductedatthefloweringstage.Theresultsrevealedsignificantvariationsinnetphotosyntheticrate(Pn),transpirationrate(E),transpirationefficiency(Pn/E)andcarboxylationefficiency(Pn/Ci).PnshowedsignificantpositivecorrelationwithE,stomatalconductance(gs),Pn/Ciandtotalcanopydrymatter.SpecificleafareaandleafthicknesswerenotsignificantlycorrelatedwithPn.Thirty-sevenoutof40ILsshowedhigherPnthanKMR3[11.28μmol/(m2·s)],and20ILsshowedhigherPnthanWR120[15.08μmol/(m2·s)].ThelineIL194showedthehighestPn[21.62μmol/(m2·s)]withincreasedtotalcanopydrymatterfollowedbylinesIL381,IL106,IL363-12,IL198,IL86-18andIL50,whichexhibitedPnabove18.0μmol/(m2·s).TheILswithenhancedPnareapotentialsourcefordevelopingricevarietiesandhybridswithhigherbiomassandyield.

简介：由202根线组成的一张回交重组体近交系人口从XieqingzaoB//XieqingzaoB/Dongxiang野米饭被开发。人口在植物hopper抵抗和收益特点上是assayedwithDNA标记和phenotyped。A连锁图在于of119脱氧核糖核酸标记并且跨越了因为在12个米饭染色体上的1188厘米被构造。Thirty-twochromosomal片断替换线在标记loci基于XieqingzaoB等位基因的百分比被选择。这些线具有为基因印射和相异的基因基因渗入的大潜力。

简介：105根转基因的米饭线的一个总数独立地与豌豆含铁听基因(Fer)转变了以前被获得。在selfing和贝它-glucuronidase(GUS)的七代帮助了选择以后，有稳定的农学的特点的82根转基因的线被得到。在82根转基因的线，二高铁的转基因的米饭线Fer34和Fer65之中，与在milled的铁内容，是4.82和3.46野生型Xiushui11的预定，分别地被识别。在二根转基因的线，每基因外长高度被表示，并且继承为一个单个地点。transgene没在米饭植物的农学的特点上有否定效果，另外的矿物质营养的部件，外观质量和milled的吃的质量，显示这二根线是精英高铁的繁殖线。而且，实际申请和引起线的二精英的进一步的研究便于利用被讨论。

简介：Roll-leaf-1(rl-1)andspot-leaf-1(spl-1)weretwonear-isogeniclines,whichwereobtainedafter3to4backcrosseswithearlyseasonindicariceZhefu802asrecurrentparent.Hennamacro-lesions,referredasphysiologicalormorphologicalmarkers,begantoappearonleavesat4.5-to6.0-leafstage.Thericeseedlingswereinoculatedat3.5-,5.0-and7.0-leafstageswithhighpathogenicracesZhongA1andZhongB1ofMagnaporthegrisea,respectively.Theresistanceofrl-1,spl-1andZhefu802againstblastwassignificantlydifferent.TheseedlingsofZhefu802at3.5-to7.0-leafstageweresusceptibletoracesZhongA1andZhongB1ofM.grisea,whereasthoseofrl-1andspl-1at3.5-,5.0-and7.0-leafstagesweresusceptible,moderatelyresistantandresistant,respectively.Theseresultssuggestedthattheenhancedresistanceofri-1andspl-1relatedtotheappearanceoftheirmorphologicalmarkerlesions.Theexperimentprovidedabasisforstudyinglesionmimicandhypersensitiveresponseinassociationwithdiseaseresistance.

简介：二细菌的老家(BB)抵抗基因，Xa21和Xa4，从IRBB24被介绍进杂交稻restorer线Mianhui725，它高度产生BB，由使用杂交和分子的帮助标记的选择技术。四根相应恢复系线通过与分子的标记测试R目标基因并且分析父母遗传背景被获得。有特定的紧张ofXanthomonasoryzaepv的四根线和他们的混血儿的接种。oryzae，P1，P6和七个代表汉语拉紧病理型，CⅠ-CⅦ，证明所有四根线和他们的混血儿是到BB的高度抵抗、介绍的宽广抵抗光谱。G46A/R207-2的混血儿显示了好农学的人物和高产潜力，并且R207-2被称为Shuhui207。

简介：为phosphoenolpyruvatecarboxylase基因(ZmC4Ppc)的未经触动的Zeamays基因全身是6781bp。为这基因的PCR的产品不与差的重覆性是清楚的，结果因为它在米饭和玉米两个都对帮助标记的选择(妈)困难。为为妈选择标记，仅仅在玉米而非在米饭介绍的序列被强风识别，并且用教材首相5.0为教材设计使用了。一双特定的教材称为MRpc(向前：5AAGCAGGGAAGCGAGACG3，逆行：5GATTGCCGCCAGCAGTAG3)被用于转变米饭的选择，并且ZmC4Ppc能高度并且组成地在使用MRpc选择的转变米饭在每个测试发展阶段被表示。因此，MRpc被用于与ZmC4Ppc在米饭和一些restorer线带ZmC4Ppc基因的子孙的妈(例如FPM881)与restorer线Shuhui881源于转变ZmC4Ppc的Kitaakebackcrossed被获得。基因背景上的分析，PEPCase活动，捕捞光合的率，一般联合能力(GCA)和显示出的FPM881的特定的联合能力(SCA)超过95%到达的基因背景的那类似，PEPCase和网络光合的率比那些高控制，并且一些带ZmC4Ppc基因的子孙为谷物的GCA和SCA最好每植物让步，控制比那些每植物，和1000谷物重量圆锥花序数。这建议经由妈和它的稳定的表示的玉米ZmC4Ppc基因的介绍能增加米饭的谷物产量并且将多半为米饭提供一条小径品种的改进。

简介：Introgressionlines(ILs)derivedfrominterspecificcrossesareasourceofnewgeneticvariability.Atotalof55ILsderivedfromtwocrossesSwarna×O.nivaraIRGC81848(populationA)andSwarna×O.nivaraIRGC81832(populationB)werecharacterizedforyieldandyield-relatedtraits/QTLs.Segregationof103simplesequencerepeat(SSR)markersassociatedwithyield-relatedQTLswasstudied.PopulationAshowedanaverageof12.6%homozygousO.nivaraallelesandpopulationBshowed10.6%.Interestingly,threeSSRmarkers,RM223,RM128andRM517,showedconspicuouspatternofsegregation.ThedistributionofparentalallelesatthreelociRM223,RM128andRM517linkedtoyield-relatedtraitswasunique.Thesemarkersflankedtoseveralyield-relatedQTLs.RM223,flankingtoqyld8.3,washeterozygousinalmostallthe55ILsexceptinIL10-3SandIL131S.RM128onchromosome1andRM517onchromosome3weremutuallyexclusivein46outof55ILs.These46ILsshowedeitherofthemarkeralleleRM128orRM517fromO.nivarabutnotboth.IL166ShadbothRM128andRM517fromO.nivaraandtheotherILsshowedhomozygousSwarnaalleleatRM517exceptIL65S.Populationstructureassignedthe55ILstothreesub-populationsbasedontheirgenomicdiversity.IL65S,IL166S,IL248S,IL7KandIL250Kshowedhighyieldsinmulti-locationtrials,andIL248SwasreleasedforcultivationasDRRDhan40.

简介：干旱是到在rainfed低地的米饭生产的主要不能生活的限制并且不足地灌溉区域。干旱的改进容忍的变化是策略之一减少干旱的否定效果。为与染色体上的干旱忍耐(DT)有关的主要、第二等的特点的量的特点loci(QTL)1，3，4，8和9从线从在CT9993和IR62266之间的一个十字导出的两倍haploid决定了那是introgressed并且在Khao邮政马里105的基因背景(KDML105)把了进小片开发染色体片断替换线(CSSL)人口。CSSL在干旱应力下面为他们的农学的表演和收益部件在繁殖阶段被评估，并且结果与灌溉状况相比。CSSL线的flowering比KDML105早是6～7d。在CSSL的谷物收益的吝啬的价值比在干旱下面的KDML105高并且灌溉了条件。以灌溉状况，谷物从4和8是的染色体带DT-QTLs的基因渗入线让步比KDML105的高，而另外的特点与KDML105显示出小差别。当否定地与天相关到flowering时，分析显示谷物产量在干旱应力下面每植物每植物，和全部的谷物重量与植物高度，tiller和圆锥花序数字有积极关联。是提及在上面，在干旱应力下面显示出好改编的CSSL能被用作基因材料，并且作为材料在引起节目的泰国rainfed低地米饭改进干旱忍耐把基因位于\O下面干旱忍耐。