简介:Bt5198,包含Bt基因的一根新米饭restorer线,与BtMinghui63从精英restorer线Chenghui177的十字和回交被开发,一根转基因的Btrestorer线。生来的线用PCR扩大,测试纸评估,在实验室的昆虫抵抗评估和稻地被评估,大米强风抵抗的托儿所评估和农学的特点的家谱选择。当米饭灰煤杆在实验室与有斑纹的茎borer(SSB)的鸡蛋被接种时,Bt5198和BtMinghui63上的幼虫的死亡是100%。Bt5198在地条件下面对SSB和黄茎borer(YSB)是高度抵抗的。F1混血儿源于Bt5198和四细胞质的男性无菌(厘米)线对SSB和YSB也高度抵抗并且有重要杂种优势。米饭强风抵抗的二年的评估BtMinghui63比那些更好证实到叶的Bt5198的抵抗层次爆炸,颈强风类似于Chenghui177并且显著地的那些。Bt5198的种子萌芽能力和花粉产量与Chenghui177是类似的,建议进新restorer线的Bt基因的介绍没有种子活力或种子生产的收益上的重要效果。识别Bt基因的存在,把测试纸检查与昆虫抵抗的评估相结合是有效的,在实验室并且在地条件下面。
简介:WhenbeingplantedinwideareasinsouthernChina(23°23'-33°23'N,98°35’-129°19'E,sealevelabove2.7-1285m),Liangyoupeijiu(LYPJ),atwo-linehybridricecombination,showedaseedsettingrateof75.2-77.2%,whichwaslowerby4.3-7.5percentpointthanthatofanindicahybridriceShanyou63(CK),withsimilarvaluesofgrainyieldandcoefficientsofvariationtoCK.Sowingduring5-25thofMayinNanjing(32°3'N.118°48'E),China,LYPJheadedbefore4thSeptember,andgaveaseedsettingrateof75-90%,andgrainyieldover1kg/m^2.Ifthesowingdatewasdelayedto14-15th,June,itsheadingdatewouldbeaslateas17th-21stSeptember,andseedsettingratewouldbedeclinedby10-15%incomparisonwiththatonasuitablesowingdate.Whenfloweringtookplaceatanaveragedailytemperaturerangeof13.7-28.5℃forfivedays,thespikeletfertility(SF)wouldbeincreasedby1.9-10.7%,foreachincrementof1℃.Thesuitable(SF≥90%)andsafe(SF≥75%)temperaturesforfloweringstagewereindicatedtobe26.5°and22.9°,respectively.TogetahighandstableseedsetincultivatingLYPJ,itwasrecommendedthatLYPJwouldbeplantedintheareassouthof34°N,andthefavorableaveragedailytemperatureduringfloweringstageshouldbeat26-28℃.
简介:与较普通活字大一倍的杂交稻Yangliangyou6(YLY6)和Liangyoupeijiu(LYPJ),andthree线杂交稻Shanyou63(SY63)作为与谷物充满联合的材料,来源,水池和流动特征被调查。种子背景率,谷物充满度和YLY6和SY63的谷物产量比LYPJ的那些显著地高。在YLY6和SY63的灰煤杆和鞘的事的出口和转变百分比比LYPJ的那些显著地高。在谷物的蔗糖synthase,腺苷diphosphoglucosepyrophosphorylase,淀粉synthase和淀粉分叉酶的活动比为LYPJ为YLY6andSY63是更高的,并且很显著地与充满率,吝啬的谷物充满率,谷物充满度和粒重的最大的谷物被相关。每维管束的区域和YLY6和SY63的韧皮部的小穗状花小穗数字,谷物收益和全部的水池负担是比LYPJ的那些显著地小的,并且越大,越多并且越多降低种子背景率负担更差的谷物充满。交通率每YLY6的区域韧皮部比LYPJ或SY63的大。Theresults建议YLY6拥有强壮的来源,大水池活动和有效流动,它为它充满的高种子背景率和好谷物放了一个生理的底。
简介:Thetransgenicrice,Zhongda2,whichwasgeneticallymodifiedfromanindicaricelineZhuxianBbyricechitinasegene(RC24),hadhighresistancetoricesheathblight(Rhizoctoniasolam)inlaboratoryandatwo-yearfieldexperiment.ThepathogencouldinvadesheathofZhongda2andinducesymptomsofthedisease.NodifferencewasnotedintimeofpenetrationorincubationperiodbetweenZhongda2andnon-transgenicricecontrol,ZhuxianB,butthehyphaelysatecouldbeobservedeadierthancontrol.Itsresistanceexpressedastoinhibitthegrowthofmyceliuminhosttissue.F1sfromZhongda2(♂)crossedwithotherfivenon-transgenicricelinesshowedhigherresistancethandonornon-transgenicparents,buttheresistancewasdifferentalongwiththedifferentmaternalparents.
简介:Themajormatesterilegenesinanewphoto/thermo-sensitivegenicmalesterile(PTGMS)lineB06Sofricewereanalyzedbythemanipulationofmixturedistributiontheory.TheresultsindicatedthatapairofmajormalesterilenucleargeneswithlargeeffectswereresponsibleforcontrollingthemalesterilityofB06S.
简介:YuetaiA的种子样品,一种Honglian(HL)类型细胞质的男性无菌(厘米)在混合大米的线被调查估计种子纯净并且由在南京,江苏省和Lingshui模仿生物沾染物到YuetaiA分析离开类型植物的原因,海南省在20042006期间。YuetaiA的种子杂质主要源于在生物污染以后然而并非从它的绝育unstability恢复富饶基因(Rf)的基因飘移。十根维护者线,五根restorer线和三thermo敏感的遗传因子的男性,无菌的线在学习使用了的所有能传花粉给生产F1的YuetaiA和YuetaiB种,直接或间接地导致漂流进YuetaiA并且产生iso细胞质恢复产生的Rf基因。而且,高outcrossing率和有许多变化的YuetaiA的类似的标题日期容易在米饭生产力量使用了在生物污染的结果。在移开所有植物,Rf基因在YuetaiA混合了并且阻止Rf基因漂流进YuetaiA以后,YuetaiA和YuetaiB的种子纯净被提起了到100%。
简介:在米饭restorer线C224的条纹疾病抵抗的继承为量的特点加多基因用主要基因的混合效果模型被分析。另外,抵抗与维护者线在C224的七个十字被调查。结果证明C224的条纹抵抗被二主要基因与添加剂优势效果(E-1模型)加多基因与additive-dominance-epistasis效果控制。这二基因有12.47%和24.75%分别地,出现的否定优势效果的添加剂效果。在二主要基因之间有重要epistasis和相互作用效果。当多基因的是2.74%时,二主要基因的可遗传性是92.12%,显示条纹抵抗有主导的主要基因效果。七个十字,五显示了高或中等的抵抗到条纹疾病。
简介:Nearisogeniclinescarryinglarge-effectQTL(qtl12.1),whichhasaconsistentinfluenceongrainyieldunderuplanddroughtstressconditionsinawiderangeofenvironments,wereevaluatedunderwaterstressinthefields.Thelinewhichgavehigheryieldunderdroughtwascrossedwithalocaleliteline,PMK3,andforwardedtoF2:3generation.SignificantvariationwasfoundamongtheF2:3linesforagronomictraitsunderwaterstressinthefields.Lowtohighbroadsenseheritability(H)forinvestigatedtraitswasalsofound.Waterstressindicatorssuchasleafrollingandleafdryingwerenegativelycorrelatedwithplantheight,biomassandgrainyieldunderstress.Bulkedsegregantanalysis(BSA)wasperformedwiththemarkersinthevicinityofqtl12.1,andRM27933wasfoundtobesegregatedperfectlywellinindividualcomponentsofdroughtresistantanddroughtsusceptiblebulkswhichwerebulkedbasedonyieldunderwaterstressamongF2:3lines.Hence,thissimpleandbreederfriendlymarker,RM27933,maybeusefulasapotentiallyvaluablecandidatemarkerforthetransferoftheQTLqtl12.1intheregionalbreedingprogram.BioinformaticanalysisoftheDNAsequenceoftheqtl12.1regionwasalsodonetoidentifyandanalyzepositionalcandidategenesassociatedwiththisQTLandtoascertaintheputativemolecularbasisofqtl12.1.