简介:Inducedembryonicstemresourcesfortheobservationofthecell(ES)cellsareexpectedtobepromisingcellbehaviorsindevelopmentalbiologyaswellastheimplantationincelltreatmentsinhumandiseases.ArecombinantE-cadherinsubstratumwasdevelopedasacellrecognizablesubstratumtomaintaintheEScells'self-renewalandpluripotencyatsinglecelllevel.Furthermore,thegenerationofvariouscelllineagesindifferentgermlayers,includinghepaticorneuralcells,wasachievedonthechimericproteinlayerpreciselyandeffectively.Theinductionandisolationofspecificcellpopulationwascarriedoutwiththeenhancingeffectofotherartificialextracellularmatrices(ECMs)inenzyme-freeprocess.ThemurineEScell-derivedcellsshowedhighlymorphologicalsimilaritiesandfunctionalexpressionstomaturedhepatocytesorneuralprogenitorcells.
简介:ConstantloadtestsinNS4solutionpurgedwithN2-5%CO2gasmixturewereconductedonAmericanPetroleumInstitute(API)X80pipelinesteelappliedinthe2ndWest-EastGasPipelineprojectwithandwithoutpreload.TheresultsshowthatcrackscouldinitiateandpropagateinX80pipelinesteelinnear-neutralpHenvironmentunderaconstantloadcondition.Thelifeofcrackinitiationandpropagationincreasedwithdecreasingappliedstress.Preloaddidnotchangeitscorrosionbehaviorobviously.However,preloadreducedthetimeforcrackinitiation.