简介：Weinvestigatedtheeffectofphotodynamictherapy(PDT)withhematoporphyrinmonomethylether(HMME)ontheviabilityofStreptococcusmutans(S.mutans)cellsonbiofilmsinvitro.Streptococcusmutansistheprimaryetiologicalagentofhumandentalcaries.Sincedentalcariesarelocalizedinfections,suchplaque-relateddiseaseswouldbewellsuitedtoPDT.Thediodelaserusedinthisstudyhadthewavelengthof635nm,whoseoutputpowerwas10mWandtheenergydensitywas12.74J/cm~2.HMMEwasusedasphotosensitizer.Sampleswerepreparedanddividedintofivegroups:(1)HMME;(2)Laser;(3)HMME+Laser;(4)Controlgroup(+)withchlorhexidine;and(5)Controlgroup(-)withsterilephysiologicalsaline.InoculumofS.mutansincubatedwithHMMEalsoexaminedwithfluorescencemicroscopy.PDTexhibitedasignificantly(P<0.05)increasedantimicrobialpotentialcomparedwith20μm/mLHMMEonly,laseronly,0.05%chlorhexidine,and0.9%sterilephysiologicalsaline,whichreducedtheS.mutansofthebiofilmmosteffectively.Laserand0.05%chlorhexidinewerecausedreductionintheviablecountsofS.mutanssignificantlydifferent(P<0.05)also,butthesetwotesttreatmentsdidnotstatisticallydifferfromeachother.HMMEgroupdidnotstatisticallydifferwithnegativecontrolgroup.FluorescencemicroscopyindicatedthatHMMElocalizedprimarilyintheS.mutansofthebiofilm.ItwasdemonstratedthatHMME-mediatedPDTwasefficientatkillingS.mutansofbiofilmsandausefulapproachinthetreatmentofdentalplaque-relateddiseases.

简介：Toinvestigatetheeffectofphotodynamictherapy(PDT)withhematoporphrinmonomethylether(HMME)onbovineimmunodeficiencyvirus(BIV)canprovidethebasistheoryforphotoinactivationofhumanimmunodeficiencyvirus(HIV).ToassesstheprotectionofHMME-PDTonthecelllineCf2ThinfectedwithBIVR29by3-(4,5)-dimethylthiahiazol-2-yl-3,5-di-phenytetrazoliumbromide(MTT)withpowerdensityof5and25mW/cm~2andenergydensityfrom0.6to3J/cm~2.ToobservetheinhibitionofmembranefusionusinganewreportercelllineBIVEbyfluorescencemicroscope.HMME-PDThassignificantprotectanteffectsonCf2Th-BIVR29withbothpowerdensities,especiallyinthegroupofhighpowerdensity.FluorescentmicroscopeshowsthatthereisnosignificantdifferencebetweenthegroupofPDTandcontrol,whichmeansPDTcouldnotinhibittheBIV-mediatedmembranefusion.