简介：AThepurposeofthisinvestigationwastostudythetherapeuticeffectofLamivudineonHBVDNAinperipheralbloodmononuclearcells(PBMC)andserum,andthelevelofcytokinesinserumofthepatientswithchronichepatitisB.Thepatientsweredividedintotwogroups(A=47,B=34),andtreatedbyLamivudine,routinemedicine,respectively.ThelevelsofHBV-DNAinPBMCandserumandcytokineswerealldetectedbeforeandaftertreatment.AfterthetreatmentofLamivndinefor36weeks,thetotalconversionnegativeratesofHBV-DNAinPBMCandserumofthepatientstreatedwithLamivudinewere55.32%(26/47)and61.70%(29/47),respectively.ThetotalnegativeconversionratesofHBV-DNAinPBMCandserumofthepatientstreatedbyroutinemedicinewere26.47%(9/34)and32.35%(11/34),respectively.TherewassignificantdifferencebetweenLamivudinegroupandroutinemedicinegroup(P<0.01).ThenegativeconversionratesofHBeAginserumofthepatientswere46.81%(22/47)and68.09%(32/47)attheendof24weeksand36weeks,andwerehigherthanthoseofroutinemedicinegroup(P<0.05andP<0.01).Thelevelsofalanineaminotransferase(ALT),aspartateaminotransferase(AST),ALT/ASTinserumofthepatientsafterbeingtreatedbyLamivudine,routinemedicineweredown-regulatedto(30.1±9.6)U/ml,(32.3±10.7)U/ml,0.9±0.1and(48.4±10.7)U/ml,(44.7±11.0)U/ml,1.1±0.2.Aftertheanalysisofvariance,thehighsignificantdifferencewasobviousbetweenthetwogroups(P<0.01).ItwasduetothehighlevelsofIL-6,IL-8andTNF-αinchronichepatitisBwhichcouldbedown-regulatedto(250.5±33.3)pg/ml,(153.4±22.2)pg/ml,(232.6±21.2)pg/mlbyLamivudine,whichwasmoreobviousthanthatofroutinemedicine(P<0.01).LamivudinehashightherapeuticeffectonthetreatmentofHBVDNAinPBMCandserumandhasbettertherapeuticeffectthanthatofroutinetherapy.Lamivudinemayalsohavehigherdown-regulatedinflammatoryinfiltrationandsecretioninlocalsitecaused

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简介：ObjectiveToExplorethemechanismoftheacupunctureonposanenopausalosteoporo-sis.MethodOnehundredWistarfemalerats(6month-old)wererandomlydividedintotwogroups:O-variectomizedgroupandsham-operatedgroup.After3monthsofoperation,threeratswerechosentomeasurethebonehistomorphometricindexes.Thentheovariectomizedratsweredistributedrandomlytoacupuncturegroupandyigaininggroupandnormalsalinegroupandcontrol2group,theratswererespec-tivelytreatedbyacupunctureandyigainingandnormalsaline.Afterthetreatmentforthreecourse,allratswerecollectedbloodfromtheateriafemoralis,thelevelsofcalcitoninweremeasuredbyIRMA.thebonehistomorphometricindexesandC-cellinthyroidfollicleweredetected.ResultsThelevelsofcalcitoninofacupuncturegroupwereincreasingsignificantlycomparingwiththeyigaininggroupandthenormalsalinegroupandthecontrol2group(p<0.05),buttherewerenoevidentdifferenceinthelevelsofcal-citoninbetweentheacupuncturegroupandthesham-operatedgroup.Themeantrabecularbonewidthandthetmbecuharbonevolume%ofacupuncturegroupwereincreasingmorethanthatofthenormalsalinegroupandthecontrol2group(p<0.05);themeanmarrowcavitywidthandthemarrowcavityvol-ume%werederreasingmorethanthatofthenomalsalinegroupandthecontrol2group(P<0.05);thenumberofC-cellinthyroidfollicleoftheacupuncturegroupwereincreasingmorethanthatoftheothergroupsexceptthesham-operatedgroup.ConclusionAcupuncturecaneffectivelytreatthepost-menopausalosteoporosisbystrengtheningtheabilityoftheC-cellinthyroidfolliclewhichcanimprovethelevelsofcalcitonininserum.

简介：Thequantumvibrationalenergy-spectraincludinghighexcitedstatesoftheproteinmoleculeshavebeencalculatedbynewtheoryofbio-energytransportalongtheproteinmoleculesanditsdynamicequation,discretenonlinearSchrodingerequation,appropriatetotheproteinmoleculesonthebasisofthelevelofmolecularstructure.Thisenergy-spectraobtainedarebasicallyconsistentwiththeexperimentalvaluesbyinfraredabsorptionandradiatedmeasurementofperson'shandsandlaser-RamanspectrumfrommetabolicallyactiveE.Coli..Fromthisenergy-spectraweknowthattheinfraredlightswith(1-3)x1000nmand(5-7)x1000nmwavelengthcanbeabsorbedbytheproteinmoleculesinthelivingsystems.Inaccordancewiththenon-lineartheoryofthebio-energytransportweknowthattheenergyoftheinfraredlightabsorbedbytheproteinscanresultinvibrationsofamide-Iinaminoacidsandcanfacilitatethebio-energytransportalongtheproteinmolecularchainsfromoneplacetootherforthegrowthoflivingbodies.Thisprocesseisnon-thermal.Thisisjustnon-thermaleffectoftheinfraredlights.Accordingtothemechanismweexplainedfurtherthemedicalfunctionsoftheinfraredlightsabsorbed.

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简介：目的：观察针刺治疗黄褐斑的疗效。方法：将90例患者随机分为治疗组和对照组，每组各45例。其中，治疗组又辨证分成3型，予针刺治疗。对照组予维生素C和维生素E治疗。结果：治疗组和对照组有效率分别为93.3％和73．3％(P<0．05)，而治疗组内各型疗效无差异(P>0．05)。结论：针刺治疗各型黄竭斑均有明显的效果。

简介：Objective:Toevaluatetheassociationbetweenobesityandtheriskofcolorectalcancer.Methods:331patientswithrectalcancerand175withcoloncancerwhoacceptedsurgicaloperationatBeijingCancerHospitalduring1995and2002wereenrolled.Datawerecollectedbyreviewingthepathologymaterialsandhospitalrecords.258healthypeoplewhoacceptedhealthexaminationatBeijingCancerHospitalduring2000and2002werealsoenrolledascontrol.Dataofheight,weightandgenderatthetimeofexaminationwerealsocollected.Obesitywasestimatedbybodymassindex(BMI),computedasweightinkilogramsdividedbyheightinmeterssquared(kg/m2).ThedegreeofobesitywascomparedbetweenthetwogroupsusingBMI(18.5,24-27.9and(28(kg/m2)asthecut-offpointsforunderweight,overweightandobesity.Associationswithobesitywereestimatedbyoddsratios(ORs)and95%confidenceintervals(CIs).AllORswereadjustedforageandsex.Results:Obesitywassignificantlyprevalentinfemalepatientswithrectalcancer.AllthepatientswithcoloncancershowedlowerlevelofBMIthancontrolsubjects.TheORsforrectalcancerrosewithincreasingBMIinwomen.Meanwhile,theORsforcoloncancerdroppedwithincreasingBMIinbothmenandwomen.Obesitywasanindependentriskfactorforrectalcancer,butnotanindependentriskfactorforcoloncancer.Conclusion:Rectalcancerandcoloncancermayhavedifferentbiologicalbehavior.Obesewomenhaverelativelyhighriskforrectalcancer.

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简介：Objective:Toevaluatetheefficacyandsignificanceofposterolateralfusioninpreventingfailureofshort-segmentstabilizationforthetreatmentofthoracolumbarburstfractures.Methods:Sixtypatientswiththoracolumbarburstfractureswereincludedinthestudy.Thepatientswereclassifiedintotwogroups(n=30ineachgroup).InGroupA,patientsweretreatedinourhospitalwithshort-segmentinstrumentationviaposterolateralfusionwithiliacbone.InGroupB,patientsweretreatedinotherhospitalwithshort-segmentfixationwithoutfusion.Allcasescametoourhospitalforreexamination.Therewere18malesand12femalesinGroupAwithameanageof42.3years(range,24to52years)and16malesand14femalesinGroupBwithameanageof41.5years(range,19to54years).Radiographic(Cobbangle,kyphosisofthevertebralbody,andsagittalindex)andclinicaloutcomes(LowBackOutcomeScore)wereanalyzedafteranaveragefollow-upof16months.Results:Afteroperation,Cobbanglewasreducedfrom19.3°to3.1°inGroupAandfrom19.1°to3.3°inGroupB(P>0.05).Itwas5.9°inGroupAand11.9°inGroupBatthefinalfollow-up(P<0.01).Itsaveragelossofcorrectionwas2.8°inGroupAand8.6°inGroupB.Averagekyphosisofthevertebralbodywasreducedfrom21.3°to6.2°inGroupAandfrom21.7°to7.4°inGroupB(P>0.05).Itwasdecreasedto7.9°inGroupAand13.5°inGroupBatthefinalfollow-up(P<0.01).Itsaveragelossofcorrectionwas1.7°inGroupAand6.1°inGroupB.Sagittalindexwasreducedfrom21.3°to3.6°inGroupAandfrom20.5°to3.8°inGroupB(P<0.05).Itwasdecreasedto5.1°inGroupAand9.8°inGroupBatthefinalfollow-up(P<0.01).Itsaveragelosswas1.5°inGroupAand6.0°inGroupB.InGroupA,73.3%ofpatientshadanexcellentresultbasedonLowBackOutcomeScoresystem,whilethatinGroupBwasonly43.3%.Conclusions:Posterolateralfusionisaneffectivemeasuretopreventimplantfailure,anddecreaselo

简介：精子是导出把父亲的单倍体染色体送到卵母细胞的一种高度专业化的房间类型。渐成说，或基因规章，精子的性质和机制在父亲的染色体的准备帮助贡献胚胎开始和接合子的染色体。许多最近的研究在精子处理了改变的渐成说过程的问题。这评论评估DNA损坏，染色体非整倍性，减少的端粒长度，中心体的畸形性，印错误的genomic，改变的mRNA侧面，并且在胚胎开始上的在授精和观察效果以前的在精子的反常原子包装的当前的理解。注意也被给了在人与改变的渐成说机制理解精子的内在的病原学。

简介：BackgroundMyocardialfibrosisisoneprocessofthevariousheartdiseases,whichleadstocommonpathologicalchangeswhenitdevelopstoacertainstage.Itisthemaincauseofventricularremodelingwhicheventuallyleadstodifferentdegreesofcardiacdysfunction,malignantarrhythmiasandsuddencardiacdeath.Manystudieshaveshownthatvariouscytokinesplayaveryimportantroleinthedevelopmentofmyocardialfibrosis.Thispaperreviewsthelatestresearchesontheroleofcytokinesinmyocardialfibrosis.

简介：Osteocytesactasmechanosensorsinbone,whichcansendmechanicalsignalsdirectlytoosteoblaststhroughgapjunctions.However,underphysiologicalconditionsthenumberofgapjunctionsislimitedbecauseofthequantityvarianceofthetwokindsofcells.Inthisstudy,thepossibilityofindirectinteractionbetweenthesetwocellswasinvestigated.Anewflowchamperwasdesignedinwhichosteocytesandosteoblastswerecoculturedintwochampersseparately.OsteocyteswereexposedtofluidflowandthentheALPactivity,osteocalcinandosteopotinofosteoblastsweredetermined.TheresultsshowedthateitherALPactivityorproductionofosteocalcinandosteopotininosteoblaststhatwerecoculturedwithshearedosteocytesincreased,whichindicatedthatosteocytescouldregulateosteoblastsindirectlythroughsomesolublefactors.

简介：ObjectiveToinvestigatetheeffectoffamilytherapyonprognosisofpatientswithschizophrenis.Method200patientsafterdischargingfromhospitalwererandomlydividedintotwogroups:controlgroup(n=100)andexperimentgroup(n=100).Familytherapywasactualizedinpatientsofexperimentgroup.ResultsTherewassignificantdifferenceinpercentofrecurrenceratebetweencontrolgroup(38%)andexperimentgroup(18%,P<0.05).ConclusionFamilytherapymaydecreaserecurrencerateofschizophrenicafterdischargingfromhospital.

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简介：Objective:Toobservetheeffectofacupunctureonimagesinautismchildren.Methods:Atotalof27casesofautismchildrenweresubjectedintothisstudy.ByusingaSPECT,thecerebralimageswerecollectedbeforeandafteracupuncturetreatmentandanalyzedaccordingtotherecommendedmethodsinforassessingthestateofbloodflow,radioactivityquantitydistributionandradioactivitycountinbilateralhemispheres.'JIN'sthree-needling'wasemployed.Theacupuncturetreatmentwasgivenonceeveryotherday,with4monthsbeingatherapeuticcourseandanintervalofonemonthbetweentwocourses.Results:Afteracupuncturetreatment,ofthe22cases,20hadremarkableimprovementand2hadimprovementincerebralbloodflow,withthetotaleffectiverateofimprovingcerebralbloodflowbeing90.8%.Beforethetreatmentthereweresignificantdifferencesbetweentheleftandrightcerebrum(P<0.001),andbetweentheleftandrightfrontallobesinradioactiveareas(P<0.01);however,aftertreatment,nodifferenceswerefoundbetweenthem(P>0.05).Aftertreatment,theradioactivitycountinthewholebraindecreasedsignificantlyincomparisonwiththatofpretreatment(P<0.01).Itindicatestheimprovementofcerebralbloodflowandcellularmetabolismafterthetreatment.Conclusion:Acupumctureconsignificantlyimprovecerebralbloodflowinautismchildren.

简介：Objectives:Toexploretheeffectofacupunctureoncerebralinfarctioninratsandtotryprovidingsomeexperimentalparametersforclinicalpractice.Methods:27healthyWistarratswererandomlydividedintopseudo-operation(n=10),model(n=8)andacupuncture(n=9)groups.Neuro-functionaldefectscoring,apoptosisofsinglebrainsliceandthenumberofbcl-2immuno-reaction(IR)-positiveneuronsinCA1areaofthehippocampuswereusedastheindexestoinvestigatethepossiblemechanismsofacupunctureof'NieSanZhen'(needlingthreeacupointsinthetemporalregion)and'SiShenZhen'(needlingfouracupointsintheocciputregion)intreatingratswithcerebralinfarction.Results:Thereexistedsignificantdifferencesbetweenacupuncturegroupandmodelgroupinimprovingneurologicfunctionalactivities,inhibitingapoptosisofthebraincellsandincreasingbcl-2IR-positiveneuronsinthehippocampalCA1area(P＜0.01).Conclusion:Acupuncturetherapycanimprovecerebralinfarctionintheratbysuppressingapoptosisandup-regulationofthebcl-2IR-positiveneuronexpression.

简介：Objective:Toobservetheeffectofacupunctureonhyperleptinaemiaandhyperinsulinemiaforstudyingitsunderlyingmechanismaboutanti-obesityandreducingbloodlipidinobesityrats.Methods:Atotalof80SDratswererandomizedintonormalcontrol,model,acupunctureandmedicationgroups,with20casesineachgroup.Hypothalamicobesitymodelwasestablishedbysubcutaneousinjectionof15%sodiumglutamate(0.2mL/10gbodyweight),oncedailyandcontinuouslyfor5days.'Zusanli'(足三里ST36),'Sanyinjiao'(三阴交SP6),'Guanyuan'(关元CV4)and'Zhongwan'(中脘CV12)werepuncturedandstimulatedelectrically(100Hz,dense-sparsewaves,andasuitablestrengthinducinglocalmusculartremor)for15min,oncedaily.Inmedicationgroup,ratswerefedwithSibutramine4mg/kg,oncedaily.After4weeks'treatment,Lee'sindexwasdetected,andserumleptinandinsulincontentsweredeterminedbyradioimmunoassay(RIA).Results:Comparedwithnormalcontrolgroup,Lee'sindex,serumleptinandinsulincontentsofmodelgroupincreasedsignificantly(P<0.01).Comparisonbetweenacupunctureandmodelgroups,Lee'sindexandserumleptinofacupuncturegroupdecreasedsignificantly(P<0.01),andseruminsulinlevelalsolowered.Incomparisonwithmodelgroup,Lee'sindex,serumleptinandinsulinlevelsofmedicationgroupalsolowered.ComparisonbetweenacupunctureandmedicationgroupsshowedthatLee'sindexandserumleptinlevelofacupuncturegroupweresignificantlylowerthanthoseofthelatergroup(P<0.01),displayingthatthetherapeuticeffectofacupunctureisbetterthanthatofSibutramineinloweringLee'sindexandserumleptin.Nosignificantdifferenceswerefoundbetweenacupunctureandmedicationgroupsinbodyweightandlengthandseruminsulin(P>0.05).Conclusion:AcupuncturecaneffectivelyreduceLee'sindex,serumleptinandinsulincontentsinfastingobeserats,whichmaycontributetoitseffectinanti-obesity.

简介：ObjectivesToinvestigatetheprotectiveeffectofthrombopoietin(TPO)onmyocardialcellsinvitro.MethodsH9C2celllinewasmaintainedinIscove’smodifiedDulbecco’smedium(IMDM)supplementedwith10%calfserum.Beatingcellsfromheartventriclesofneonatalheartwereculturedataninvitrosystem.Apoptosisofthecelllineabovewasinducedbytreatmentofdoxorubicin(DOX)andwasblockedbyTPO.CellsurvivalrateofH9C2cellwasmeasuredbytheMTTassay.Changesofbeatingrateofneonatalmyocardialcellswerecapturedbydigitalcameraandbeatingratewascalculated.Flowcytometrywasemployedtostudyanti-apoptoticeffectofTPObystainingJC-1proteintoH9C2cell.ResultsMTTassaydemonstratedthatdoxorubicinreducedcellsurvivalrateby73.8%±1.1%,50ng·mL-1and100ng·mL-1TPOincreasedcellsurvivalrateby84.6%±3.6%(P<0.05),86%±4%(P<0.01)atadose-dependentmanner.Beatingrateofprimaryneonatalmyocardialcellsalsodecreasedto15%±8%at48h,100ng·mL-1TPOimprovedbeatingrateto48%±11%(P<0.01).TPOdecreasedapoptoticratefrom19%±9%to11%±6%(P<0.05).ConclusionsTPOhasprotectiveeffectonmyocardialcellsinvitro.Anti-apoptosisisoneofthemechanismsbywhichTPOprotectsinjuredheart.

简介：Aim:ToevaluatetheeffectofaqueousCaricapapayaseedextractonmicroenvironmentofcaudaepididymis.Methods:Adultmalealbinoratswereintrauscularlyadministeredwith0(control)or0.5mgpapayaseedextract/kgbodyweightfor7days.Caudaepididymaltubularcontentwascollectedbymicropuncturetechnique;epididyrealluminalfluidandspermpelletswereseparatelyanalyzed.Results:Theresultsrevealedthattheextracttreatmentcausedsignificantreduction,ascomparedwithcontrol,intotalproteinandsialicacidcontentsinbothepididymalfluidandspermpellet.Ascomparedwithcontrol,significantlyloweredacidphosphataseactivitywasrecordedinspermpelletbutwashigherinepididymalfluidafterthetreatment.TheextracttreatmentalsocausedsignificantreductioninlevelofinorganicphosphorusintheePididymalfluid.Conclusion:Itisconcludedthattheaqueouspapayaseedextractalterscaudaepididymalmicroenvironment.

简介：客观5-Aminoisoquinolinone，一水溶性，多形核白细胞(腺苷5'-diphosphate核糖)的活动的有势力禁止者聚合酶，在禁止多形核白细胞(腺苷5'-diphosphate核糖)的活动与ischaemia-reperfusion损害和发炎联系的织物损害起一个重要作用聚合酶和象ICAM-1那样的房间粘附分子的表示，P-selectin等。但是不在肿瘤它怎么样是清楚的。现在的学习的目的是在对人的脐的静脉endothelial的冒号癌线HT-29房间的粘附上学习5-Aminoisoquinolinon的效果；并且在结肠癌HT-29房间的ICAM-1，P-selectin和多形核白细胞(腺苷5'-diphosphate核糖)的活动聚合酶的表示上的5-Aminoisoquinolinon的效果。到人的脐的静脉endothelial的HT-29房间的粘附被粘合剂实验检测的方法。Immunocytochemically，Streptavidin-Peroxidase方法被用来调查ICAM-1，P-selectin和多形核白细胞(腺苷5'-diphosphate核糖)的表示(多形核白细胞(腺苷5'-diphosphate核糖)的产品聚合酶激活)。到HUVEC的HT-29房间的粘附试金的结果显示出的结果在每个5-AIQ-treated组的OD570价值是重要的，这以一种剂量依赖者方式在控制组(5-AIQ-untreated)比那降低。ICAM-1，P-selectin和多形核白细胞(腺苷5'-diphosphate核糖)的表示是重要的在HT-29房间在5-Aminoisoquinolinone-untreated组比那组织的5-Aminoisoquinolinone-treated更低。结论数据建议5-Aminoisoquinolinone能禁止HT-29房间的粘附到人的脐的静脉endothelial。5-Aminoisoquinolinone能也禁止多形核白细胞(腺苷5'-diphosphate核糖)聚合酶激活和在HT-29房间的ICAM-1和P-selectin的表情。5-Aminoisoquinolinone可能贡献肿瘤房间转移的预防。进一步的学习被需要。