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简介：Themitochondrial12SrRNAhasbeenshowntobethehotspotformutationsassociatedwithbothaminoglycoside-inducedandnon-syndromichearingloss.Ofallthemutations,thehomoplasmicA1555GandC1494Tmutationsatahighlyconserveddecodingregioninthe12SrRNAhavebeenassociatedwithaminoglycoside-inducedandnon-syndromichearinglossinmanyfamiliesworldwide.TheA1555GorC1494Tmutationisexpectedtoformnovel1494C-G1555or1494U-A1555base-pairatthehighlyconservedA-siteof12SrRNA.ThesetransitionsmakethesecondarystructureofthisRNAmorecloselyresemblethecorrespondingregionofbacterial16SrRNA.Thus,thenewU-AorG-Cpairin12SrRNAcreatedbytheC1494TorA1555Gtransitionfacilitatesthebindingofaminoglycosides,therebyaccountingforthefactthattheexposuretoaminoglycosidescaninduceorworsenhearinglossinindividualscarryingthesemutations.Furthermore,thegrowthdefectandimpairmentofmitochondrialtranslationwereobservedincelllinescarryingtheA1555GorC1494Tmutationinthepresenceofhighconcentrationofaminoglycosides.Inaddition,nuclearmodifiergenesandmitochondrialhaplotypesmodulatethephenotypicmanifestationoftheA1555GandC1494Tmutations.TheseobservationsprovidethedirectgeneticandbiochemicalevidencesthattheA1555GorC1494TmutationisapathogenicmtDNAmutationassociatedwithaminoglycoside-inducedandnonsyndromichearingloss.Therefore,thesedatahavebeenprovidingvaluableinformationandtechnologytopredictwhichindividualsareatriskforototoxicity,toimprovethesafetyofaminoglycosideantibiotictherapy,andeventuallytodecreasetheincidenceofdeafness.

简介：硬皮病是一种自身免疫性疾病，其特征为早期炎症性浸润，进而发展为皮肤及内脏纤维化。CREST是硬皮病的一种相对良性皮肤变异，特点为钙质沉着、雷诺现象、食管功能异常、指端硬化和毛细血管扩张。CREST很少伴发肾小球肾炎。作者报道1例CREST患者发生肾小球肾炎，并在其血清中检出抗着丝点抗体和抗中性粒细胞胞浆抗体（ANCA）。

简介：ＳＴＵＤＹＯＮＭＥＴＡＳＴＡＳＩＳＡＳＳＯＣＩＡＴＥＤＧＥＮＥＳＣＲＥＥＮＥＤＢＹＭＯＮＯＣＬＯＮＡＬＡＮＴＩＢＯＤＹＨＩＬＱｉＴｅｎｇｐｉｎｇ齐藤平；Ｚｈａｎｇｐｅｉｊｉ张沛基；ＷｅｉＳｈｕｇｕａｎｇ魏曙光；ＣｈｅｎＤｏｎｇ陈东；ＬｉＲｅｎ李仁；Ｗ...

简介：Theadenomatoidodontogenictumorischaracterizedbytheformationofduct-likestructuresandappearanceofeosinophilicmaterials.Eosinophilicmaterialsshowamorphousmassordropletsamongtheepithelialcells.Intheduct-likestructures,itshowsathinlayerofbasementmembranel...

简介：Objective:Toinvestigatetheapproachofemergencymanagementforseverepelvicfractureassociatedwithinjuriesofadjacentvisceraandevaluatethetherapeuticeffect.Methods:Thedataof79patientswithseverepelvicfractureassociatedwithinjuriesofadjacentviscerawereretrospectivelystudied,andthestudycoveredaperiodof14years.Results:Toceasemassivebleedingduetopelvicfracture,ligationofinternaliliacarterieswasperformedon33cases,andangioembolizationon8.Of42patientswithcysticor/andurethralinjury,35underwentcystostomyanddelayedreconstruction,and7receivedaprimaryrealignment.Allof17patientswithinjuryofretroperitonealrectumunderwentdivertingcolostomyoftheproximalendofsigmoidwithpresacraldrainage,but4receivedprimaryrepairwithoutcolostomy.In22patientswithintraperitonealcolorectalinjury,19weremanagedwithprimaryrepairoranastomosiswhile3receivedacolostomy.Theoverallmortalityratewas8.86%(7/79);themaincauseswerehemorrhagicshockandassociatedinjury.Thecomplicationsincludedurethro-rectalfistulain4cases,thrombosisofrightcommoniliacarteryin1,ARDSfollowingchesttraumain1,andparaplegiain1.Exceptthepatientwithparaplegia,allofthemwerecured.Conclusions:Promptdiagnosisandpropertreatmentarethekeytosuccess.Devascularizationofinternaliliacarterieswithexternalfixationcageofthepelvis,cystostomyandproximalsigmoidostomyareeffectiveproceduresinemergencytreatmentofthecriticalpatients.

简介：ＡＲｅｓｅａｒｃｈＩｎｓｔｉｔｕｔｅｏｆＳｕｒｇｅｒｙ，ＤａｐｉｎｇＨｏｓｐｉｔａｌ，ＴｈｉｒｄＭｉｌｉｔａｒｙＭｅｄｉｃａｌＵｎｉｖｅｒｓｉｔｙ，Ｃｈｏｎｇｑｉｎｇ４０００４２，Ｃｈｉｎａ（ＬｉｕＬＭ，ＣｈｅｎＨＳ，ＨｕＤＹ，ＬｕＲＱ，ＣｈｅｎＹ...

简介：LongbeforethediscoveryofHelicobacterpylori,thereweremanyexcellentobservationalstudiesthatdocumenteddifferencesinthepatternsofgastroduodenaldisease.Itwasclearthatinthedevelopingworld,gastriculcerandgastriccancerweremorecommonthaninthedevelopedworldwhereduodenalulcerpredominated.Thiscorrelatedwiththedistributionofgastritisinduodenalulcerpatientswheretheinflammationwasantralpredominantwhileingastriculcerpatientsthegastritiswasmoreevenlydistributedthroughthestomach.Gastriculcersusuallyappearedinafairlyrestricteddistributioninthestomachneartheangulusandclosetothetransitionalzonebetweenantrumandbodymucosa.Asasocietydevelopedsothesepatternsofdiseasechanged.

简介：患者女，19岁。既往有青霉素、头孢菌素皮试阳性史。因出现心悸、多汗、易饥饿和体重减轻症状，于2005年6月在当地医院诊断为甲状腺功能亢进症（甲亢），给予丙硫氧嘧啶（PTU）150mg-200mg／d，酒石酸美托洛尔（倍他乐克）25mg／d，口服。2006年4月28日患者出现感冒症状并伴有发热（T38．5℃）。双上肢可见暗红色、大小不等的出血性皮疹，部分融合成片。4d后皮疹消退，但开始出现咳痰，偶有痰中带血丝。于2006年5月8日收住院。查体：甲状腺Ⅱ度肿大，可扪及震颤，闻及血管杂音。突眼度左19mm，右18mm。双肺呼吸音清，未闻及干、湿性啰音。

简介：Objective:TumorassociatedantigenencodinggeneHCA520(AF146019)wasidentifiedbyscreeningahumanhepatocellularcarcinomaexpressingcDNAlibraryusingSEREXtechnique.InthisexperimentwestudiedtheeffectofHCA520oncellproliferationandapoptosis.Methods:GeneHCA520wasgainedbyPCRandtransfectedinto293cells.ThestableexpressioncellswereobtainedbyG418selection.Thecellproliferationwasmeasuredby[3H]-TdRuptakeandapoptosisassaywasmeasuredbyFACS.Results:EukaryoticexpressionplasmidpcDNA3-HCA520wasconstructedanditsstabletransfectantswereobtained.OverexpressionofHCA520inhibitedthecellproliferationandenhancedcellapoptosisafterserumdeprivation.Conclusion:HCA520isanoveltumorassociatedantigenthatcanaffectcellproliferationandapoptosis.

简介：AIMToexpressandpurifyLipoprotein-associatedphospholipaseA2(Lp-PLA2),andtoestablishascreeningmodelforLp-PLA2inhibitorsthroughtherecombinantLp-PLA2.METHODSThefull-lengthgeneofLp-PLA2wasclonedfromthedifferentiatedTHP-1cellsbyRT-PCRandPCR.TheLp-PLA2genewassubclonedintothePichiaexpressionvectorpPIC9andintroducedasequenceencodingaC-terminalstretchofsixhistidineresiduesatthesametime.TherecombinantplasmidwastransformedintoPichiapastorisGS115byspheroplastingandthegenewasthenintegratedintotheGS115genome.Lp-PLA2wasexpressedintheyeaststrainGS115byinducingwith0.5%methanol.

简介：TheeffectofPentoxifylline(PTX)ontype1diabeteswasinvestigatedbymeansofthestudiesontheexpressionsofcytokinemRNAinpancreasandtheFas-Fasl,onisletcellsofNODmice.NODmiceweretreatedwithFFXfrom4-6wk,andthenfrom8-12wk.Aftertreatment,itwasfoundthattheincidenceofdiabetesinNODmiceatagesof30wkwasreducedto25%ingroupofmicetreatedwithPTX,incomparisonto73.3%incaseofmiceinjectedwithPBS,andthedegreeofinsulitisinthePTXtreatedmicewaslowerthanthatofthePBSinjectedmice.RT-PCRanalysisrevealeddown-regulatoryeffectontheexpressionsofIFN-γandTNF-αmRNAinPTXtreatedmice,buttherewasnoanyeffectontheexpressionofIL-10.AstotheexpressionofFas,therewasmarkeddecreaseinthemeancytoplasmicintegralopticaldensity(IOD)inPTXtreatedmice,buttherewaslittledifferencebetweenFIXandPBSgroupsintheexpressionofFasL.TheseresultsindicatedthatFIXcouldpreventthedevelopmentofdiabetesinNODmice,whichmightberelatedtotheregulationofTh1/Th2imbalanceandthedecreasedexpressionofFasinisletcells.

简介：调查瘤的目的淋巴的联系转移的基因；它的分子的机制。方法22690鼠标染色体cDNA微数组(包括14500知道基因；4371EST)被用来比较；分析老鼠肝细胞癌房间线Hca-F(高度淋巴的转移潜力)的基因表示侧面；Hca-P(低潜力)。结果901基因；129EST至少是起来调整的在Hca-F的2褶层房间。在表示显示出重要改变的33基因被介绍，包括endoglin(EDG)，MCAM，Cdc42ep5，F2r，D7Ertd458e，Serpinh1(HSP47)，AXL，Areg；那么上。这些基因有血管生成的函数，房间粘附，信号转导变异，房间活动性，女伴活动，蛋白质激酶活动；受体绑定。微数组与淋巴的转移模型相结合的结论cDNA可能贡献新方法；淋巴的转移研究的瘤的线索。在表示基因上的一些可能提供新奇线索给瘤的分子的机制淋巴的转移。

简介：ObjectivesTostudythephenotypeofaChinaLQTfamilyandinvestigatetherelationshipofphenotypeandgene.MethodsTheclinicalmaterialswereanalyzedandgenemutationswerescreenedbysequencing.ResultsAdistinctivebiphasicTwavepatternwasshownintheleftprecordialleadsofallpatients.TheLQT2relatedHERGgeneAla561Valmutationwasfound.ConclusionsAprolongedQTintervalaccompaniedbiphasicTwaveindicatesHERGmutation.

简介：TheaimofthisstudyistoexplorepotentialpathogenicityofMycoplasmapenetrans,andtoinvestigatewhetherM.penetranslipid-associatedmembraneproteins(LAMPs)couldinducehumanmonocyticcellline(THP-1)toproducesomeproinflammatorycytokinesinvitro,includinginterleukin-1β(IL-1β),tumornecrosisfactoralpha(TNF-α),andIL-8.THP-1wasstimulatedwithdifferentconcentrationsofM.penetransLAMPsandatdifferenttimetoanalyzetheproductionofhumanIL-1β,TNF-αandIL-8.TheproteinlevelsofhumanIL-1β,TNF-αandIL-8weremeasuredbyenzyme-linkedimmunoadsorbentassay(ELISA)andthemRNAlevelsoftheseproinflamrnatorycytokinesweredetectedbyreversetranscriptase-PCR(RT-PCR).ItwasdemonstratedinthepresentstudythattheproductionofIL-1β,TNF-αandIL-8increasedindose-andtime-dependentmannerafterstimulationwithM.penetransLAMPsinTHP-1cells.M.penetransLAMPsalsoinducedtheexpressionofIL-1β,TNF-αandIL-8mRNA.TheproductionofIL-1β,TNF-αandIL-8andtheexpressionofmRNAweredown-regulatedbypyrrolidinedithiocarbamate(PDTC).ThisstudydemonstratedthatM.penetransLAMPscaninducetheproductionofproinflammatorycytokinesinhumanmonocyticcellsinvitro,thussuggestingthatitmaybeanimportantetiologicalfactor.

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简介：客观：为了与M-CSFR验证MAF-J6-1受体的抗原协会并且进一步学习M-CSF和它的受体的角色，调停了在支持白血病的房间增长的juxtacrine。方法：MAF-J6-1RRE2的Monoclonal抗体(McAb)和rhM-CSFR的polyclonal抗体(PolyAb)被准备。到M-CSFR的McAbRE2的特性被ELISA被间接ELISA，有J6-1房间殖民地形成的跨neutralizing试金和中立化测试证实。结果：到M-CSFR的净化的RE2的反应活动是超过1：16000。M-CSFR和MAF-J6-1R的禁止的活动能被RE2和anti-M-CSFR抗体堵住。到M-CSFR的RE2的反应能被M-CSFR减少。结论：到M-CSFR的RE2的特性被证实，有M-CSFR的MAF-J6-1R的抗原协会被证明。它建议M-CSF和它的受体调停了auto-juxtacrine刺激能是在白血病或nonhematological恶意的起作用的机制。

简介：瞄准：为了在ischemia-reperfusion(红外)上调查rosuvastatin的保护的效果，并且在内皮的氮的氧化物synthase(eNOS)的表示上决定这个代理人的效果，在老鼠导致了小肠的损害和发炎蛋白质。方法：肠的损坏被为30min夹钳优异mes伤寒动脉和腹的箱子在男Sprague-Dawley老鼠导致，为60min由灌注列在后面。在生理盐水溶解的Rosuvastatinintraperitoneally被管理在局部缺血前的60min。肠的粘膜损害和发炎的严厉被几个生物化学的标记，以及由组织检查所见评估。eNOS的蛋白质层次被西方的污点决定。结果：当粘膜的索引损坏，管腔内血红素和蛋白质的层次显著地在假冒操作组与那些相比在红外组被增加。然而，这些增加被处理显著地以一种剂量依赖者方式与rosuvastatin禁止。rosuvastatin的保护的效果被组织检查所见也证实。到红外的小肠的暴露导致了thiobarbituric的重要增加描绘的粘膜发炎酸反应的物质，联系织物的myeloperoxidase活动，和老鼠的粘膜内容导致cytokine的嗜中性的chemoattractant-1(CINC-1)和肿瘤坏死factor-alpha(TNF-alpha)。在红外以后的煽动性的参数的这些增加被预告的处理显著地在10mg/kg的剂量与rosuvastatin禁止。而且，CINC-1和TNF-alpha的mRNA表示在红外以后被增加，并且这增加被rosuvastatin也禁止。eNOS的粘膜蛋白质层次在红外期间减少了，但是在与rosuvastatin对待的老鼠被保存。结论：Rosuvastatin禁止老鼠红外导致的肠的损害和发炎，和它的保护与eNOS的保藏被联系蛋白质。

简介：Objective:Toinvestigatethepreparationofthecarboxymethlydextranironoxidemagneticnanoparticles(CDMN)andtheeffectsofCDMNcarriersystemassociatedwithexternalmagneticfieldsonkillingtumorcellsandgenetransfectioninvitro.Methods:Epirubicin-CDMN(EPI-CDMN)andgreenfluorescentprotein(GFP)plasmid-CDMN(GFP-CDMN)werepreparedbytheoxidation-reductionprocedureandtheircharactersweredetected,respectively.TheeffectsofEPI-CDMNassociatedwithexternalpulsedelectromagneticfields(PEMFs)(10mT)onkillinghumanbladdercancerBIU-87cellswerestudiedbyMTTassayandAnnexin-V/PIdouble-labeledflowcytometrytechnique,respectively.AndthetransfectionefficiencyofGFPwhenCDMNwereusedasgenecarrierassociatedwiththeexternalmagneticfieldswasevaluatedunderfluorescencemicroscopeinvitro.Results:ThediameterofEPI-CDMNandGFP-CDMNwereabout8~10nmand5~9nm,respectively,andsaturationmagnetizationwere0.22emu/gand0.26emu/g,respectively.EPI-CDMNassociatedwithPEMFscouldsignificantlyinhibittheproliferationofBIU-87cellsandinducecellsapoptosis,thegrowthinhibitoryrateandapoptosisratewere(21.82(3.18)%and(16.79(3.37)%,respectively.ThetransfectionefficiencyofGFP-CDMNcombinedwithPEMFswassignificanthigherthanthatofGFP-CDMNwithoutPEMFs[(45.70(4.32)%vs(35.85(2.16)%,P<0.05].Conclusion:ItseemedthatEPI-CDMNassociatedwithexternalmagneticfieldscouldsignificantlykilledhumanbladdercancerBIU-87cellsandCDMNcouldeffectivelytransferGFPgeneintotumorscellswiththehelpofexternalmagneticfieldswhichprovidedexperimentalbasisforthemagnetictargetingtherapyoftumor.

简介：ToinvestigatetheprotectiveeffectofdecoctionofliaoweiongastricmucosaofH.pyloriassociatedchronicatrophicgastritisinrats.TheH.pyloriassociatedchronicatrophicgastritismodelswereinducedwithsodiumdeoxycholate,sodiumsalicylate,ethanol,andculturebrothofH.pylori.ProtectiveeffectofdecoctionofliaoweiongastricmucosaofH.pyloriassociatedchronicatrophicgastritiswasobservedquantitatively.