简介：Thispaperpresentsanovelmethodofspotaddressingandsegmentationabouttheforegroundsegmentationofmicroarrayimage.Inthispaper,aspotaddressingmethodbasedonparticleswarmoptimization(PSO),algorithmisproposedtohaveafurthersearchforthecentercoordinateandradiusofthespotwhoseregionisdeterminedbytheprojectionmethod.Then,aforegroundsegmentationmethodisputforwardtomakethespotforegroundsegmentationbasedonthecentercoordinateandradiusofthespot.Thespotaddressingandsegmentationexperimentsonsyntheticandrealmicroarrayimagesshowthattheproposedmethodiseffectiveandfeasiblefortheforegroundsegmentationofmicroarrayimage.

简介：Theergonomicdigitaldesignandevaluationofman-machinesystemisthemainapplicationfieldsofvirtualhumantechnology.Becauseofthecomplexityofphysicalpersonanduncertaintyoffatiguegenerationmechanism,itisdifficulttoexpressandevaluatetheVirtualHuman'sfatiguestate.Thispapersummarizesthecharacteristicsofvirtualhuman'sfatigueevaluation,suchasundeterminable,invisible,theconstraintconditionscanbeenused.Viewedfromthestrengthsandweaknessesofpreexistingmodels,metabolicenergyexpendituremodel,dynamicmusclefatiguemodelandamodeltoassessfatigueatjoint-levelusingthehalf-jointconcept,thispaperputsforwardthefuturedevelopmenttrendthatthecombinationofsubjectiveandobjective,diversificationoftheanalyzablemotion,real-timeandvisualexpression.

简介：Inthispaper,weproposeamethodtoimproveanti-radiationcapabilitybycoatingheavymetalX-rayprotectionglasswithcompoundphotoniccrystallayers,basedontheuniquepropertyofphotoniccrystalthatlightcannotbepropagatedwithintherangeofbandgaps.Usingtheplanewaveexpansionmethod,wemadeatheoreticalstudyofparametersaffectingthebandgapstructuresofone-dimensionalphotoniccrystals.Basedonthefindings,wechoseappropriatematerialsandcompoundstructureofphotoniccrystalsoastogethighX-rayreflectioncoatingphotoniccrystallayers.Bythismethod,thereflectionratewithinX-raywavelengthcanreachthemaximumvalueof100%,andtheaveragevalueofover90%.Evenlow-costheavymetalX-rayprotectionglassofabsorptioncoefficientvaluecanachievethedesiredeffect.Thus,thismethodgreatlydecreasestheanti-radiationrequirementsofheavyX-rayprotectionglass.

简介：Objective:ThisstudyaimstoestablishamethodforhighlyparallelmultiplexeddetectionofgeneticmutationsinChineselungcancersamplesthroughAgenaiPLEXchemistryandmatrix-assistedlaserdesorptionionizationtime-of-flightanalysisonMassARRAYmassspectrometryplatform.Methods:Wereviewedtherelatedliteratureanddataonlungcancertreatments.Wealsoidentified99mutationhotspotsin13targetgenescloselyrelatedtothepathogenesis,drugresistance,andmetastasisoflungcancer.Atotalof297primers,composedof99pairedforwardandreverseamplificationprimersand99matchedextensionprimers,weredesignedusingAssayDesignsoftware.Thedetectionmethodwasestablishedbyanalyzingeightcelllinesandsixlungcancerspecimens.TheproposedmethodwasthenvalidatedthroughcomparisonsbyusingaLungCarta~(TM)kit.ThesensitivityandspecificityoftheproposedmethodwereevaluatedbydirectlysequencingEGFRandKRASgenesin100lungcancercases.Results:Theproposedmethodwasabletodetectmultiplexgeneticmutationsinlungcancercelllines.Thisfindingwasconsistentwiththeobservationsonpreviouslyreportedmutations.Theproposedmethodcanalsodetectsuchmutationsinclinicallungcancerspecimens.ThisresultwasconsistentwiththeobservationswithLungCarta~(TM)kit.However,anFGFR2mutationwasdetectedonlythroughtheproposedmethod.Themeasuredsensitivityandspecificitywere100%and96.3%,respectively.Conclusions:TheproposedMassARRAYtechnology-basedmultiplexmethodcandetectgeneticmutationsinChineselungcancerpatients.Therefore,theproposedmethodcanbeappliedtodetectmutationsinothercancertissues.