简介:ComparedwiththespeciesofLeucesection,thedifficultyinplantregenerationduringtissuecultureforthespeciesofAigeirossectionisakeylimitingfactorfortheirapplicationingeneticengineeringofforesttrees.Inthisstudy,theplantgrowthregulatorscombination,copperconcentration,lightintensity,andtheselectionpressuresofkanamycinwereinvestigatedusingleavesofPopulus×euramericanacl.’Bofeng’asexplants,andastableandhighefficientregenerationsystemwasestablished.Usingthissystem,boththeshootregenerationrateandrootingratewereupto100%,andtheaveragenumberofdifferentiatedshootsineachleafexplantwasupto20,andthesurvivalrateofseedlingsreached98%.Theoptimalselectionpressureofkanamycininshootinducementforleaf-explantwas40mg·L-1,andtheoptimalselectionpressureofkanamycininrootinducementofadventitiousbudwas20mg·L-1.TheauthorsalsofoundthatdifferentiationrateofadventitiousbudsofP.×euramericanacl.’Bofeng’couldbesignificantlypromotedbyincreasedcopperconcentration,andthisisthefirstreportaboutthekeyroleofcopper(Cu)inexplantregenerationofPoplars.ThisregenerationsystemwithhighfrequencywhichwasestablishedbytheauthorswasasgoodasthatofspeciesofLeucesection,anditprovidesagoodtechnologicalplatformforgeneticengineeringofforesttreewhichusesthespeciesofAigeirossectionasmodelmaterials.
简介:Low-molecular-weight(LMW)organicacidsexistwidelyinsoilsandhavebeenimplicatedinmanysoilprocesses.TheobjectiveofthepresentpaperwastoevaluateeffectoftwoLMWorganicacids,citricacidandoxalicacid,onCl-adsorptionbythreevariablechargesoils,alatosol,alateriticredsoilandaredsoil,usingabatchmethod.TheresultsshowedthatthepresenceofcitricacidandoxalicacidledtoadecreaseinCl-adsorptionwithlargerdecreasesforcitricacid.AmongthedifferentsoilsGl-adsorptioninthelateriticredsoilandtheredsoilwasmoreaffectedbyboththeLMWorganicacidsthanthatinthelatosol.
简介:本研究以转BADH-反义4Cl二色胡枝子(L.bicolor)为研究对象,对其进行抗盐性试验。通过在茎段增殖培养基添加不同浓度的NaCl(0g/L,2g/L,4g/L,6g/L,8g/L)和生根培养基中添加不同浓度的NaC(0g/L,1g/L,2g/L,3g/L,4g/L),对转lBADH-反义4Cl基因的不同株系,进行茎段增殖和生根培养。结果表明培养基中添加2g/LNaCl不能抑制生根,适合作为生根阶段的选择剂浓度。当NaCl浓度达到4g/L时茎段分化率低下,该浓度可作为适合茎段分化的选择剂浓度。NaCl浓度大于6g/L就抑制了茎段分化,NaCl浓度大于3g/L浓度就抑制了根的生长。两个浓度为胡枝子的筛选浓度或极限浓度。综合茎段增殖系数、平均分化芽数量等测定因素,22号株系在转入双基因BADH-反义4Cl之后耐盐性相对较高。综合生根率、平均生根数量等因素,74号株系在转入双基因BADH-反义4Cl之后耐盐性相对较高。本实验旨在为转基因胡枝子的育种提供一定的理论基础。