简介:Thelight-harvestingchlorophylla/b-proteincomplexplaysanimportantroleinphotosynthesisofplants.Afull-lengthcDNAoflight-harvestingchlorophylla/b(cab)genewasclonedfromthefirststrandofMoso(Phyllostachysedulis)cDNAthroughRT-PCRandRACEmethods,namedascab-PhE10(cabgene10fromPh.edulis).Thelengthofcab-PhE10(GenBankaccessionnumber:EU118754)is1151bp,whichcontainsanopenreadingframeencoding283aminoacidsfrom81stto932ndposition.Thebioinformaticsanalysisindicatedthattheproteinencodedbycab-PhE10hadachlorophlla/bbindingdomain(83rd-247thposition),twoproteinkinaseC-phosphorylationsites,threeN-myristoylationsitesandayiaA/Bdoublehelixdomain.Theaminoacidsequenceofcab-PhE10showedhighsimilaritywiththecabgenesofOryzasativa,Zeamays,Hordeumvulgare,andVitisvinifera,morethan80%,respectively,whichindicatedthatcab-PhE10genebelongstolhcb5genefamily.