简介:背景:SOD1-G93A转基因小鼠是研究肌萎缩侧索硬化的经典代表性疾病模型动物,然而对于这种转基因小鼠在保种过程中所存在的种系退化现象却少有报道。目的:研究家族性肌萎缩侧索硬化小鼠在保种过程中出现的种系退化现象。方法:通过比较家族性肌萎缩侧索硬化疾病模型SOD1-G93A转基因小鼠在保种过程中不同代间的怀孕胎数、产仔数以及子代鼠中雄性和阳性小鼠所占得比例分析该转基因小鼠保种过程中生育能力的变化,并比较不同代间小鼠的起病时间、生存期以分析该转基因小鼠在保种过程中发病情况的变化。结果与结论:第3,6代小鼠与第0代比较,其怀孕胎数、每胎产仔数、每只产仔数等均显著下降(P〈0.05),且第6代小鼠中有一半以上雌鼠无法受孕。其子代鼠中雄性小鼠以及阳性小鼠所占的比例有下降趋势,但是其差异无显著性意义。第6代小鼠与第1代比较其发病时间与死亡时间分别推迟了6.37d(P=-0.004)和9.67d(P=0.022),差异有显著性意义。以上结果显示家族性肌萎缩侧索硬化小鼠在保种过程中存在生育能力下降、发病及死亡时间推迟等种系退化现象。
简介:为探讨坡耕地土壤退化程度及其与坡度的关系,在沂蒙山区选择典型小流域,通过测定流域内不同坡度坡耕地、弃耕地与生态林地的土壤颗粒组成、分形维数、土壤养分质量分数的变化及其相关性,研究坡耕地土壤颗粒结构与养分退化特征。结果表明:1)所有土地利用类型,土壤砂粒体积分数最高,黏粒体积分数最低,平均值分别为70.81%、0.75%;2)土壤颗粒分形维数、有机质质量分数为生态林地〉弃耕地〉坡耕地;3)坡耕地土壤颗粒分形维数随坡度增加而减小,其大小与土壤粉粒和黏粒体积分数分别呈极显著、显著正相关;4)所有土地利用类型,土壤有机质质量分数与砂粒体积分数显著负相关,与粉粒和黏粒体积分数显著正相关,土壤氮、磷、钾养分质量分数与土壤颗粒体积分数相关性不显著;5)研究区土壤具有粗骨性砂土的物理特性,坡耕地耕作或坡度加大会增强土壤粉粒和黏粒的流失,导致土壤颗粒分布的均匀性及其分维数下降,水肥保蓄性能和养分质量分数降低。
简介:侵蚀退化红壤植被恢复后生态系统碳库变化的研究对全面认识生态恢复的作用以及碳汇经营具有重要意义.试验地位于福建省长汀县河田镇,本文以采用种植百喜草治理侵蚀退化地上典型“小老头”马尾松林(百喜草治理地)为对象,以相邻的未治理地为对照,研究生态系统及其各个分室碳库的变化.结果表明:侵蚀地种植百喜草治理后生态系统、乔木层及土壤层碳库均显著(P〈0.05)或极显著(P〈0.01)高于对照地,分别是对照的2.32倍、5.23倍和1.81倍.乔木层各器官碳贮量均显著高于对照地(P〈0.05),其中树干碳贮量增量最大.与对照地土壤相比,表层0—20em土壤碳贮量增量高达5.84t·hm^-2,同时土壤深层(20~100cm)碳库增量(6.04t·hm^-2)与其相当.对照地的土壤碳库占生态系统碳库的比例为70.88%,而百喜草治理地的土壤碳库所占比例下降至55.28%,表明侵蚀地种植百喜草治理后生态系统碳库分配趋于合理.因此,从森林碳汇与可持续经营角度出发,种植百喜革治理侵蚀退化红壤是一项可行有效的措施.
简介:AIM:Tocharacterizetemporalpatternofresolutionandrecurrenceofnaivechoroidalneovascularization(CNV)secondarytowetage-relatedmaculardegeneration(AMD)treatedwithintravitrealbevacizumabonasneededregimen,andtoanalyzebaselineriskfactorsforCNVresolutionorrecurrence.METHODS:Ninety-oneeyesof80patientswithnewlydiagnosedwetAMDwereretrospectivelystudied.Alleyesweretreatedwitharoundofthreemonthlyintravitrealbevacizumabinjections,followedbyoneadditional’bonus’injectionafterresolutionofCNVactivity.Duringfollow-up,eyesweremonitoredwithfluoresceinangiography,opticalcoherencetomography,andbest-correctedvisualacuity(BCVA).IncaseofrecurrencesofCNVactivity,eyeswereretreatedwithotherroundsofbevacizumabinjectionsfollowingthesametreatmentprotocol.RESULTS:Overamedianfollow-upof532d,themedianresolutiontimeofCNVactivityinthefirst,second,andthirdtreatmentroundwas98d,126d,and111d,respectively.Themedianrecurrencetimeforthethreeroundswas154d,126d,and151d,respectively.Nosignificantdifferenceinresolutiontime(P=0.09)orinrecurrencetime(P=0.11)wasdetectedamongtreatmentrounds.Age(P=0.0082)andlensstatus(P=0.035)werefoundtobeassociatedwithCNVresolution;forevery1-yearincreaseinagetherewas4%greaterchanceofCNVresolution;Phakiceyesdemonstrateda33%betterchancetoexperienceCNVresolutionthanpseudophakiceyes.ForCNVrecurrence,lensstatus(P=0.0009)andgender(P=0.0446)werefoundtobepredictive;pseudophakiceyeshada3.69-foldgreaterrisktoexperiencerecurrenceofCNVactivitycomparedtophakiceyes;maleshada2.19-foldgreaterrisktoexperiencerecurrenceofCNVactivitythanfemales.NosignificantBCVAchangesamongthreetreatmentroundswerenoted(P=0.56).CONCLUSION:ResolutiontimeandrecurrencetimeofCNVactivitywerenotsignificantlydifferentamongtreatmentrounds,suggestingabsenceoftachyphylaxistobevacizumab.Acautiousdecisionshouldbemadeupondisco
简介:AIM:Todeterminewhethersinglenucleotidepolymorphism(SNP)rs641153isassociatedwiththeriskofage-relatedmaculardegeneration(AMD),weperformedasystematicmeta-analysisof15eligiblestudies.SNPinthecomplementfactorB(CFB)geneisconsideredtohavesignificantassociationwithAMDsusceptibility,butthereisgreatdiscrepancyintheseresults.METHODS:TheeligiblestudieswereidentifiedbysearchingthedatabasesofPubMed,EMBASE,andWebofScience.Oddsratios(ORs)with95%confidenceintervals(CIs)wereusedtoassesstheassociation.AlldatawereanalyzedusingStatasoftware.RESULTS:Theassociationbetweenrs641153andAMDriskwasstatisticallysignificantunderthehomozygousmodel(AAvsGG:OR=0.26,95%CI=0.15-0.45,P_h=0.973,/~2=0.0%,fixedeffects),dominantmodel(AA+GAvsGG:OR=0.49,95%CI=0.40-0.59,P_h=0.004,/~2=56.4%,randomeffects)andrecessivemodel(AAvsGA+GG:OR=0.30,95%CI=0.17-0.51,R_n=0.983,I~2=0.0%,fixedeffects).Thesameresultswerealsoobservedinthestratifiedanalysesbyethnicity,sourceofcontrolandsamplesize.CONCLUSION:Ourmeta-analysissuggeststhatrs641153intheCFBgenemayplayaprotectiveroleinAMDsusceptibility,thelateAMDinparticular,bothinCaucasiansandinAsians.