简介：Objectives:ToevaluatetheVidasChlamydia(CHL)assayfordetectingC.Trachomatiswithswabsandfirstcatchurine(FCU)specimensfromSTDpatientsandhighriskpopulations.Methods:Atotalof383patientsweretestedwithtissueculture(TC),VidasCHLandpolymerasechainreaction(PCR)forC.trachomatisonmaleandfemaleswabs,withVidasCHLtestingmaleFCUspecimens.CHLpositiveandequivocalresultswereconfirmedwithablockingassay(CHB).TruepositiveweredefinedaseitherTCpositive,orTCnegtivebutCHLandPCRpositive.TheperformanceofTC,CHLandPCRwereevaluatedaccordingtothisexpandedgoldstandard.Results:Comparedwiththeexpandedgoldstandard,54ofthe232malespecimensweretruepositiveresults.Formaleswabs,TC,CHLandPCRhadsensitivitiesof90.7%,96.3%and94.4%,andspecificitiesof100%,98.3%and97.2%,respectively.Differenceswerenotstatisticallysignificant.FormaleFCUspecimens,CHLsensitivityandspecificitywere83.3%and98.3%;therewaslittledifferencebetweentheseresultsandthatofmatchedswabs.Comparedwiththeexpandedgoldstandard,28ofthe151femaleswabsweretruepositive;TC,CHLandPCRhadsensitivitiesof82.1%,100%and96.4%,andspecificitiesof100%,98.4%and97.6%,respectively.Thedifferencewasalsonotsignificant.Conclusions:VidasCHLassayisverysensitiveandspecificforC.trachomatisdetectionwithswabspecimensofmaleandfemaleSTDpatients.FormaleFCUspecimens,theassayalsohadhighsensitivityandspecificity.CHBmaynotbeneededintheroutinedetectionofChlamydiainfections.PopulationswithhigherincidenceofC.trachomatisinfection.

简介：摘要目的回顾性分析VIDAS法检测高敏心肌肌钙蛋白I（hs-cTnI）在诊断“心肌梗死”中的诊断实验评价。

简介：目的调查一起食物中毒事件,分离并鉴定其致病微生物是否为大肠埃希菌O157。方法根据患者临床表现和标本种类,按照临床微生物检验法规,利用小型全自动荧光酶免疫分析仪（mini-VIDAS）、全自动细菌鉴定药敏分析系统、全自动微生物鉴定仪（VITEK32）,分别进行初筛和系统生化与药敏鉴定,并结合血清学试验作出最终鉴定。结果该起食物中毒的病原微生物被mini-VIDAS初筛为大肠埃希菌O157阳性菌株,后经系统鉴定为产毒型大肠埃希菌O8：K40,K47血清型。9份标本中有6份检出产毒型大肠埃希菌O8：K40,K47血清型,检出率为66.7%（6/9）,其中食品1份,呕吐物3份,肛拭2份,所有标本均未检出金黄色葡萄球菌、蜡样芽胞杆菌、霍乱弧菌、副溶血性弧菌、志贺菌、沙门菌及其他致泻性大肠埃希菌。结论该起食物中毒由产毒型大肠埃希菌O8：K40,K47血清型引起。产毒型大肠埃希菌（O8：K40,K47血清型）标本经mini-VIDAS初筛可以产生假阳性结果,需进一步确证。