简介:Numerouscellularfunctionsoccurinspatiallyandtemporallyconfinedregions.Recentstudieshaveshownthatmembrane-lessorganellesandcompartmentsinthecellareassembledvialiquid-liquidphaseseparation(LLPS).InvitroLLPSassaysusingrecombinantexpressedandpurifiedproteinsarenecessaryforustofurtherunderstandhowtheassemblyofphase-separatedcompartmentsisregulatedincells.However;uniformstandardsandprotocolsarelackingfortheseinvitrostudies.Here,wedescribeastep-by-stepprotocolcommonlyusedtoinvestigateinvitroLLPSusingpurifiedproteins.Thisprotocolincludesexpressionandpurificationofthestudiedproteins,inductionofLLPSofthepurifiedproteins,andstudiesofthebiophysicalpropertiesoftheliquiddropletsformedbyLLPS.TheseprotocolscanbeeasilyfollowedbyresearcherstoinvestigatetheLLPSbehaviorsofproteinsofinterest.
简介:Optofluidics是光学和microfluidics(薄片上的所谓的实验室)的集成。液体的使活动在那里是一种关键技术。在为控制microscale液体的许多方法,轻使活动特别地有趣。轻使活动提供一个新奇方法控制液体的流动为生物医学并且biotechnological应用,等等……设备的复杂性和费用可以被使用完全的光控制极大地有时减少并且可能比另外的方法在操作更灵活。然而,液体的轻使活动是象optofluidics一样的一块发芽的地。有大量工作做。这里,我们系统地基于下列点为液体的轻使活动描述四机制:optoelectrowetting,photothermal效果,放射压力,感光性的物质。
简介:相片折射的效果的一个理论处理和在液晶的nematic的联系非线性的光过程的试验性的确认被介绍。效果被归因于可以来自光电游离杂质的相片费用导致的dc空格费用域。现象被分析,在nematic液晶的相片折射度的一个理论模型被开发。新试验性的结果被发现在对理论的好同意。(编辑作者摘要)5个裁判员。
简介:Blanketsandotherin-vesselcomponentsareeasilydamagedowingtotheircircumstanceofhighradiationandhighheat.Toprotectthem,firstwalldesignshouldbeconsidered.Owingtoitshighheatremovalndself-refreshingcapability,liquidmetalfirstwallhasbeenseenasapotentialfirstwallforafusionreactorinthefuture.Blanketliquidcurtainisactuallyaspecialliquidmetalwalltoprotectblanket.
简介:Thereactionofisocyanateswithaliphaticandaromaticaminesinthel-n-butyl-3-methylimidazoliumtetrafluoroborate(bmimBF4)ionicliquidingoodtoexcellentyieldsisdescribed.Duetoitsinsolubility,thedesiredureasolidscouldberecoveredbysimplefiltrationfromtheionicliquidafterreaction.
简介:Aliquidcrystalmovingpictureprojectorandmethodaredescribed.Lightincidentonaliquidcrystaldisplay-typedeviceisselectivelyscatteredortransmittedbyrespectiveportionsofliquidcrystaldisplay,andaprojectionmechanismprojectsanimageformedbyeithersuchscatteredlightorsuchtransmittedlight.Aliquidcystalmovingpictureprojectorincludesaliquidcrystaldisplayforcreatingcharacteristicsofanimage,andprojecttionopticsforprojectingimagessequentiallycreatedbythedisplay.Thedisplayincludesaliquidcrystalmaterialcapableoftemporarystoringinformationatrespectiveareas.Thetemporarystoragemaybeafunctionofchargestoringdirectlyonliquidcrystalmaterial.Amethodofprojectingpluralimagesinsequenceincludes:creatinganimageorcharacteristicsofanimageinaliquidcrystalmaterial,storingsuchimageinsuchliquidcrystalmaterial,directinglightatsuchliquidcrystalmaterial,projectingsuchimageasafunctionoflighttransmittedthroughorscatteredbysuchliquidcrystalmaterial,andcreatingafurtherimageinsuchliquidcrystalmaterialforsubsequentprojection.
简介:UnderthesponsorshipoftheInternationalUnionofPureandAppliedChemistry(IUPAC)andChineseChemicalSociety,theInternationalConferenceonLiquidCrystalPolymerswasheldattheTwenty-FirstCenturyHotel,BeijingChina,in6-9September1994.TheconferenceChair-manandSecretarygeneralwereProfessorsQ.F.ZhouofPekingUniversityandX.J.Wangof
简介:Inthisarticletheemphasiswasgiventothediscussionoftheeffectsofdiameterratioandswirlingoninstabilitycharacterforthegas/liquidcoaxialjetusedbyLiao,etal.[1].Theresultsindicatethatthefinitediameterratiomarkedlyincreasesthemaximumgrowthrate,themostunstablewavenumber,aswellasthecutoffwavenumber.Itimpliesthatthefinitediameterratiowillleadtotheliquidjetbreakuplengthshorterandtheliquiddropsizesmaller.Theeffectoftheswirlingjetsismuchmorecomplex:fortheaxisymmetricperturbationmode,theswirlingenhancestheflowstability,forhelicalperturbation,thedominantinstabilitymodeoccursatn<0.Anditisfoundthatinlongwaveregionthereexistsanewkindofinstabilitymodesatn=1thatwasnotmentionedinLiaoetal.'sarticle.Forthisnewmode,thereappearsadominatedswirlingratioatwhichtheflowhasthemaximumgrowthrate.
简介:Heattransferinthethrustchamberisofgreatimportanceinthedesignofliquidpropellantrocketengines.Regenerativecoolingisandadvancedmethodwhichcanensurenotonlytheproperrunningbutalsohigherperformanceofarocketengine.Thetheoreticalmodeliscomplicated,itrelatestofluidbynamics,heattransfer,combustion.etc…,Inthispaper,aregenerativecoolingmodelispresented.Effectssuchasradiation,heattransfertoenvironment,variablethermalpropertiesandcokingareincludedinthemodel.Thismodelcanbeappliedtoallkindsofliquidpropellantrocketenginesaswellassimilarconstructions.Themodularizedcomputercodeiscompletedinthework.
简介:Ultrasonicattenuationinliquidhydrogenhasbeenmessuredwiththepulse-echotechniqueasafunctionoftemperaturefrom13.84Kto20.50K,at45MHz.Theresultsindicatethatthetemperaturedependenceofultrasonicattenuationinliquidhydrogenismainlydeterminedbyvolumeviscosityeffect.Ultrasonicattenuatinduetovolumeviscosityisgettingmoreandmorewithcooling.Theratiobetweenvolumeviscouscoefficientandshearviscouscoefficientisfrom1.4to4.2withinthemeasuredtempera-tureregion.
简介:Circulatingfreenucleicacids;cellfreeDNAandcirculatingmicro-RNA,arefoundintheplasmaofpatientswithhematologicandsolidmalignanciesatlevelshigherthanthatofhealthyindividuals.InpatientswithhematologicmalignancycellfreeDNAreflectstheunderlyingtumormutationalprofile,whilstmicro-RNAsreflectgeneticinterferencemechanismswithinatumorandpotentiallythesurroundingmicroenvironmentandimmuneeffectorcells.Thesecirculatingnucleicacidsofferapotentiallysimple,non-invasive,repeatableanalysisthatcanaidindiagnosis,prognosisandtherapeuticdecisionsincancertreatment.