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简介：BACKGROUND:Previousresearchhasrevealedthatsomatostatincaninduceepilepsy,andthatthelevelsofneuropeptideYmayincreaseandbecomemoreactiveinbrainareaswithepilepticseizures.OBJECTIVE:ToobservetheeffectofGanodermalucidumsporepowderontheneuropeptideYandsomatostatincontentinthecerebralcortexandhippocampalregionsofseizureratsinducedbypentylenetetrazol(PTZ).Furthermore,toverifyanyeffectofGanodermalucidumsporepowderoninhibitionofepilepticseizures.DESIGN,TIMEANDSETTING:ArandomizedgroupanimalstudywasperformedinAugust2007intheSchoolofBasicMedicalSciences,JiamusiUniversity(Jiamusi,Heilongjiang,China).MATERIALS:Thirtyhealthy,male,Wistarrats,aged12weeksandweighing180–220g,weretakenastheexperimentalanimals.PTZ(SigmaCompany,UnitedStates)wasusedtoinduceepilepsy.Ganodermalucidumspores(Leyss,exFrvariety)werepurchasedfromJiamusiCityWildGrowingCaseoftheGanodermaLucidum(China).Rabbitanti-somatostatinantibodiesandsecondaryantibodieswerepurchasedfromWuhanBosterCompany(China).NeuropeptideYradioimmunoassaykitwaspurchasedfromBeijingFuruiBiotechnologyCompany(China).METHODS:Thirtyratswererandomlydividedintothreegroups:acontrolgroup,anepilepsymodelgroupandaGanodermalucidumspore-treatedgroup.Eachgroupcontained10animals.RatsintheepilepsymodelgroupweretreatedwithintraperitonealinjectionsofPTZandgastricperfusionofphysiologicsaline.IntheGanodermalucidumspore-treatedgroup,intraperitonealinjectionofPTZandgastricperfusionofGanodermalucidumsporepowderwereadministered.Theblankcontrolgroupwasonlyadministeredwiththephysiologicalsalinebyintraperitonealinjectionandgastricperfusion.MAINOUTCOMEMEASURES:ImmunohistochemicalstainingandradioimmunoassaymethodswereusedtoobservethechangesofsomatostatinandneuropeptideYcontentinbraintissueofepilepticrats,aswellasthemorphologyofneurons

简介：ThemicewerefedwithwaterdissolveddifferentlevelY^3+(0,23.25,2325.00mg·L^-1)forsevenmonths,andthelevelsoftraceelementssuchasCu,Zn,Mn,Fe,Mg,NiandCoinbrainsofF1generationmicewerethendeterminedbyPOEMS.Theresultsshowthat,comparedwithcontrol,significantdecreasesinlevelsofFeandNi,increaseinlevelofCoareobserved,whichsuggestedthatY^3+mayhaveaneffectonthemetabolismanddistributionofsometraceelementsinbrain,andthenleadtochangesofphysiologicalfunction.