简介:为揭示大麦中黄酮合成的分子调控机制,利用反转录PCR结合同源克隆和RACE技术首次从青稞(裸大麦)叶片中克隆获得肉桂酸-4-羟化酶基因(HvC4H)的全长cDNA序列(Genbank登录号:KF927086),总长度1951bp,ORF为1518bp,编码505个氨基酸,等电点PI=9.01,平均亲水指数(GRAVY)为-0.170,属于亲水性碱性蛋白,高级结构分析表明其具有细胞色素P450家族保守域及C4H特异的功能性活性位点。利用实时荧光定量PCR分析胚乳发育5个时期不同组织(茎、叶及子粒)的表达情况,结果显示HvC4H基因在青稞胚乳发育期的表达情况存在着明显的组织差异性,在茎中的表达量最高。本研究为通过调控C4H基因的表达从而提高大麦黄酮的含量奠定了分子生物学基础,对于改良大麦的品质、抗性、生长发育等性状具有重要意义。
简介:Highenergyprotonbeamhasthelongpenetrationlengthinsolidmatteranditcouldproducetheradiographsandindicatethetomographyandmaterialpropertiesinsideofthespecimen.Consideringtheexpensivecoststoconstructahighenergyprotonaccelerator,theothercandidateofhighenergyelectronbeambecomesmoreacceptable[1??3].Duetothehighspatialandtemperalresolutionofhighenergyelectronbeam.Itisapowertooltodiagnosetheinnerstructurechangeduringthehighenergydensitymatterproductionandtheinertialconfinementfusionprocess.Inordertostudythedensityresolutionofthehighenergyelectronbeamradiography,astep-targetwasdesignedandproduced.ThestructureandthetargetpicturesareshowninFig.1andtheSitargetswithdifferentslot-widthwereproducedbyusingthemicroetchtechnics.
简介:TemperatureisoneofthekeythermodynamicparametersofHEDmatter.Asapopulartechnique,thepyrometerofnarrow-bandlterstogetherwithphotodiodes(namelymulti-channelpyrometer)hasbeenapplied.However,thepyrometercanonlymeasureone-positiontemperatureinsingleshot[1;2].Actually,itisveryimportanttoknowtemperatureevolutionofmultiplepositionsinsingleshot,especiallyforthetargetinducedbyheavyionbeam,wherealargevolumeofHEDmattercanbegenerated.Consequently,afastnew-typepyrometerhasbeendesigned.
简介:Thiscontributionreportsethylenepolymerizationbehavioroftitaniumcomplexesincorporatingbis(phenoxyimine)ligands.Sixphenoxy-imineTi(IV)complexes{6-R1-2-[CH=N(2,6-difluoro-3,5-diR2-4-R3Ph)]C6H3O}2TiCl2(1:R1=H,R2=H,R3=H;2:R1=H,R2=H,R3=4-vinylphenyl;3:R1=CH3,R2=H,R3=H;4:R1=CH3,R2=H,R3=4-vinylphenyl;5:R1=CH3,R2=F,R3=H;6:R1=CH3,R2=F,R3=4-vinylphenyl)havebeensynthesizedandevaluatedforethylenepolymerizationusingdriedMAO(simplifiedasDMAO)ascocatalyst.AnobviouscatalyticheterogeneityofCat2(Complex2/DMAO)towardsethylenepolymerizationwasobserved,whichwasillustratedbydecreasedactivity,multimodalmolecularweightdistributionandpartiallyimprovedparticlemorphologycomparingwithCat1.Moreover,Cat3exhibits'living'characteristicsintheprocessundercertainconditions(25°C,lessthan20min).Otherwise,themoderatetohighethylenepolymerizationactivityofca.105-106gPE/(molTi·h)andhighmolecularweight(Mw=105-106)ofpolyethylenecanbeobtainedbychangingtheskeletonstructureofthesecomplexes.
简介:摘要院伴随当前汽车市场的不断成熟,客户在对汽车的消费过程中产生越来越高的要求,同时也越来越严格。在经济飞速发展的今天,汽车已经成为人们生活中的必需品,同时汽车在品牌方面的归口管理需求也迫切需要汽车4S店的产生。因此,汽车4S店是一种必然的发展趋势。本文将重点对汽车销售4S店的财务核算和管理进行分析,从而为汽车4S店的高效运营提供决策参考。
简介:研究环境内分泌干扰物4-硝基酚(4-nitrophenol,PNP)对大鼠肝脏功能的毒性作用及其对核因子相关因子-2(Nrf2)通路的影响。20只SD雄性大鼠随机分成4个组,分别为对照组、1、10和100mg·kg-1体重PNP处理组,连续皮下注射28d,检测肝脏的结构变化、氧化损伤和Nrf2及其相关基因的表达情况。结果表明,与对照组相比,100mg·kg^-1PNP处理组大鼠的血清肝功能主要指标ALT、AST、AKP活性和TBIL含量显著性升高(p〈0.05),100mg·kg^-1组肝脏GSH-PX、CAT和SOD活性显著性降低(p〈0.05),大鼠肝脏中Nrf2及其下游基因NQO1和HO-1mRNA表达水平在1mg·kg^-1组显著升高(p〈0.05),10、100mg·kg^-1组有升高趋势,100mg·kg^-1组肝脏显微和超微结构都发现有不同程度的损伤。结果提示,皮下注射1mg·kg-1PNP引起了大鼠肝脏氧化损伤,机体可能通过提高Nrf2及其相关基因mRNA的表达水平来抵抗PNP引起的肝脏损伤,皮下注射100mg·kg^-1PNP改变了肝脏的正常生理功能,造成肝细胞超微结构病理损伤,引起肝脏毒性。