简介：为了学习简历，活跃代谢物由导出海绵的未耕作的共生者生产了，海绵Gelliodesgracilis的共生者的一个metagenomicDNA图书馆被构造。DNA的平均尺寸在图书馆插入是20kb。这个图书馆用纸圆盘assaying为抗菌素活动被屏蔽。二克隆对小球菌tetragenus显示了抗菌剂活动。这二克隆的代谢物通过HPLC被分析。结果证明他们的代谢物与主人E的那些相当不同。包含向量pHZ132的关口iDH5α和主人。这研究可以介绍一条新途径给探索简历海绵共生者的活跃代谢物。

简介：Inthisstudy,theentiremitochondrialDNA(mtDNA)controlregion(CR)ofPholisfangiwasamplifiedviapolymerasechainreactionfollowedbydirectsequencing.ThelengthofthemtDNACRconsensussequenceofP.fangiwas853bpinlength.Inaccordancewiththerecognitionsitesaswerepreviouslyreportedinfishspecies,themtDNACRsequenceofP.fangicanbedividedinto3domains,i.e.,theextendedterminalassociatedsequence(ETAS),thecentralconservedsequenceblock(CSB),andtheCSBdomain.Inaddition,thefollowingstructureswereidentifiedinthemtDNACRsequenceofP.fangi:2ETASsintheETASdomain(TASandcTAS),6CSBsinthecentralCSBdomain(CSB-FtoCSB-A),and3CSBsintheCSBdomain(CSB-1toCSB-3).ThesedemonstratedthatthestructureofthemtDNACRofP.fangiwassubstantiallydifferentfromthoseofmostotherfishspecies.ThemtDNACRsequenceofP.fangicontainedoneconservedregionfrom656bpto815bp.Similartomostotherfishspecies,P.fangihasnotandemrepeatsequencesinitsmtDNACRsequence.PhylogeneticanalysisbasedonthecompletemtDNACRsequencesshowedthattherewerenogeneticdifferenceswithinP.fangipopulationsofthesamegeographicaloriginandbetweenP.fangipopulationsofdifferentgeographicalorigins.

简介：Mercury(Hg)isoneofthecommonlyencounteredheavymetals,whichiswidespreadininshoresedimentsofChina.InordertoinvestigatethetoxicityofHgonmarineinvertebrates,westudiedtheeffectsofthedivalentmercuricion(Hg2+)(attwofinalconcentrationsof0.0025and0.0050mgL-1,preparedwithHgCl2)onmetallothionein(MT)content,DNAintegrity(DNAstrandbreaks)andcatalase(CAT)inthegillsandhepatopancreas,antioxidantenzymeactivitiesofsuperoxidedismutase(SOD),catalase(CAT)andglutathioneperoxidase(GPx),inthehemolymph,gillsandhepatopancreasoftheportunidcrabCharybdisjaponicaforanexperimentperiodupto15d.TheresultsindicatedthatMTwassignificantlyinducedafter3d,withapositivecorrelationwithHg2+doseandtimeinthehepatopancreasandanegativecorrelationwithHg2+doseandtimeinthegills.WhileCATinthehemolymphwasnotdetected,itincreasedinthehepatopancreasduringtheentireexperiment;SODandGPxinthethreetissueswerestimulatedafter12h,bothattainedpeakvalueandthenreducedduringtheexperimentalperiod.Meanwhile,DNAstrandbreakswereallinducedsignificantlyafter12h.TheseresultssuggestedthedetoxificationstrategiesagainstHg2+inthreetissuesofC.japonica.

简介：Aciladivaricata(Hinds,1843)andA.mirabilis(AdamsandReeve,1850)arecommonbenthicbivalvesinChina.Anumberofresearchershaveproposedthatthelatterspeciesisajuniorsynonymoftheformerspecies.Becauseofmorphologicalsimilarities,itisdifficulttodistinguishthesetwospeciesbasedonvisualexaminationonly.Forbetterunderstandingoftheirtaxonomy,themitochondrialCOIgenefragmentsoffiveindividualsofA.divaricatafromtheEastChinaSeaandsixindividualsofA.mirabilisfromtheYellowSeaweresequencedinthisstudy.ThephylogeneticrelationshipsoftheobtainedCOIsequences,togetherwithnineteensequencesofthreespeciesofthegenusNucula,wereanalyzed.Thepairwiseintra-andinter-specificdistancesfortheCOIsequencesrangedfrom0.002to0.017andfrom0.128to0.134,respectively,andnooverlapwasfound.Phylogenetically,A.divaricataandA.mirabilisformdistinctcladesandclusterintoasistertoallotherNuculaspecies.TheresultsindicatedthatA.divaricataandA.mirabilisaretwodistinctspecies.Thedifferencesinthemorphologyanddistributionbetweenthetwospecieswerebrieflydiscussed.

简介：Inthispaper,wetooktheleadinstudyingonspecificityofthemicrosatelliteDNAlociandapplicabilityofmicrosatelliteDNAprimersinprotozoa.Inordertostudycharactersofmicrosatellitesinfree-livingprotozoa,eightmicrosatellitelociprimersdevelopedfromTrypanosomacruzi(MCLE01,SCLE10,MCLE08,SCLE11,MCLF10,MCLG10,MCL03,MCL05)wereemployedtoamplifymicrosatelliteinfourfree-livingprotozoa,includingBododesignis,EuglenagracilisFACHB848,ParameciumbruziseandTetrahymenathermophilaBF1.IntheamplificationsystemsofP.bruzise,fourloci(SCLE10,SCLE11,MCLF10,MCL03)wereamplifiedsuccessfully,andfouramplificationfragmentswereinpropersize.IngenomeofE.gracilisFACHB848,fiveofeightprimersbroughtfiveclearamplificationbands.InB.designis,three(No.4,5and7)ofeightlociproducedclearandsharpproductswithoutstutterbands,whereasnobandsappearedinT.thermophilaBF1.Further,eight300-500bpamplificationfragmentswereclonedandsequenced.Nevertheless,allsequencedproductsdidnotcontaincorrespondingmicrosatellitesequence,althoughBodoisinthesameorderandhasthenearestphylogeneticrelationwithTrypanosomaamongthesefourspecies.Thus,themicrosatelliteDNAprimerscannotbeappliedamongorderormorefartaxa,andthespecificityofmicrosatelliteDNAisveryhighinprotozoa.TheresultsofthisstudywillcontributetoourunderstandingofmicrosatelliteDNAinprotozoa.

简介：Saccharina是最重要的无热水设备生活水兵褐之一海藻的类。在这研究，我们分析了S的transcriptome。装饰用的梨树，它属于1000植物(OneKP)工程，由使用定序技术的下一代的高产量的DNA。未加工的数据的大约5.16GB被产生，并且有454bp的平均长度的65536脚手架与肥皂denovo汇编方法被装配。总共，19040unigenes被强风识别；25734脚手架被聚类进37基因本体论功能的组；6760脚手架被分类进25个轮牙范畴，以及被分到306条KEGG小径的2665脚手架。unigenes的多数比另外的cyanobacteria，海洋的硅藻，和植物包括棕色的水藻和硅藻展出了更多的类似到水藻。Saccharina装饰用的梨树有突出的能力积累象Br那样的卤素，我从海水经由halogenation处理。我们在S获得了42不同钒依赖者haloperoxidases(vHPO)。装饰用的梨树transcriptome数据，包括钒依赖者iodoperoxidase(vIPO)的5个片断和钒依赖者bromoperoxidase(vBPO)的37个片断。识别fulllengthS的复杂分析。装饰用的梨树vBPO1和S。装饰用的梨树vBPO2在在海洋的海藻的vBPOs和vIPOs之间的棕色的水藻和强壮的关系的种类之中揭示了vBPO的重要性。这研究将提高我们生物特征的理解和S的经济价值。装饰用的梨树种类。

简介：综述了近10a国内外有关重金属、氨态氮、亚硝酸盐、有机污染物等几种环境因子对虾蟹类非特异性免疫影响的研究成果，以期为通过改善养殖环境条件提高虾蟹类自身免疫抗病力提供理论依据。同时，为进一步深入开展该领域的研究工作积累资料。