简介:摘要:目的膝关节置换术后康复护理。方法择取140例行膝关节置换术的患者,分析其临床资料后将其纳入研究范畴,根据所接受的护理方法进行分组,半数为常规护理组,另外半数为康复护理组。观察指标有:①膝关节置换术相关指标;②生活质量评分,③并发症数量康复护理组优势更明显,P<0.05。心理功能、躯体功能、社会功能、生活满意度评分分析,康复护理组优势更明显,P<0.05。膝关节评分分析,各组出院后三个月的指标均有明显提升,与出院时比较,P<0.05;康复护理组出院时以及出院后三个月的指标均高于常规护理组,P<0.05。并发症数量分析,康复组总计3例,少于常规护理组的7例,P<0.05。结论临床应当综合分析患者的肢体情况,适宜时积极开展快速康复护理,优化护理措施,让患者膝关节功能、疼痛感以及生存质量等都能够得到改善,在骨科临床工作中开展快速康复护理是必要的。
简介:【摘要】:目的:研究腹腔镜胃癌D2顺向式淋巴结清扫手术的治疗效果及术后炎症因子的影响。方法:在本文研究中将胃癌患者作为研究对象,200例收治时间在2020年度1月-2021年度12月,根据随机抽签分组原则分为观察组100例、对照组100例,分别采取腹腔镜胃癌D2顺向式淋巴结清扫手术方式、开腹2D根治术方式,分析并且对比两组各项指标。结果:观察组胃癌患者治疗总有效率和对照组相比,具有显著差异(P<0.05);观察组胃癌患者IL-6、MMP-9、VEGF-C水平和对照组相比,具有显著差异(P<0.05)。结论:腹腔镜胃癌D2顺向式淋巴结清扫手术的治疗效果十分显著,并且能够使患者术后机体炎症因子水平得以降低,改善疾病预后。
简介:Thepurposeofthestudyistoestablishafluorescencequantitativereversetranscriptionpoly-merasechainresponse(FQ-RT-PCR)methodforthequantitativedeterminationofIL-2mRNAandIL-4mRNAinThcells,withwhichtheThcellsstatusofthepatientswithgynaecologicaltumorsandchronicrenalfailure(CRF)canbeanalyzed.IL-2cDNAandIL-4cDNAwereprepared,andtheplasmidpMD18carryingIL-2cDNAorIL-4cDNAfragmentwasconstructedandclonedasthetemplateforquantitativedetermination.Theprimersandprobeslabelledwith6-carboxy-fluorescein(FAM)and6-carboxy-tetrarnethylrhodamine(TAMRA)wereprepared,andtheexperimentalconditionswereoptimizedtosetuptheFQ-RT-PCRmethodforquantitativedeterminationofIL-2mRNAandEL-4mRNA.Thcellsenrichedfromperipheralbloodmononuclearcells(PBMCs)of20healthyvolunteers(HVs),16gynaecologicalbenign(GB)cases,18gynaecologicalmalignant(GM)tumorcasesand16chronicrenalfailure(CRF)patientsweretestedforIL-2mRNAandIL-4mRNAbyFQ-RT-PCR.Thehouse-keepinggeneβ-actinwasusedastheinternalcontrolgeneoftheexperiment.Thestandardcurveforlogconcentrationofseriesofquantitativetemplatesvsthresholdcycle(CT)wasestablishedbylinearregression,andthelinearrangewas102-107copies/μl.TheimprecisiontestshowedtheCVofinter-assayandintra-assayofahighcontentsamplebyFQ-RT-PCRwere7.8%and12.5%,respectively.TheCVofinter-assayandintra-assayofalowcontentsamplewere10.8%and19.5%,respectively.TheIL-2mRNAexpressionsinThofthepatientswithgynaecologicalmalignanttumor(comparedwiththeHVsandthepatientswithgynaecologicalbenigndisease)andinThoftheCRFpatients(comparedwiththeHVs)weredeclinedsignificantlyandatthesametimetheIL-4mRNAexpressionincreasedsignificantly(P<0.001).Asimple,sensitiveandaccurateFQ-RT-PCRmethodforthequantitativedetectionofIL-2mRNAandIL-4mRNAhasbeenestablished.TheIL-2mRNAandIL-4m