简介：Theaggregationbehaviorandstructureofhydrophobicallymodifiedblockcopolymersofacrylamideand2-phenoxylethylacrylatewereinvestigatedbyviscometry,1HNMRrelaxation,2DNOESY,fluorescenceanddynamiclightscattering.Itwasfoundthattheaggregationbehaviorwasstronglydependentontheconcentrationofpolymersolutionandthehydrophobecontents.Withvaryingconcentrationfrom2.0,6.0,8.0to12.0g/L,thereweredifferentaggregatemorphologiesdistributedinaqueoussolutions,suchasmonopolymerchain,micelle-likeaggregate,multi-micelleaggregateandcross-linkednetwork.Accordingtothemodelofaggregation,itcangiveareasonableexplanationonthelargemagnitudeenhancementofviscositywiththeincreasingofpolymerconcentration.Additionaldataof2DNOESYandfluorescenceshowthatthecopolymerwithhigherhydrophobecontent(molarfraction≥1%)islikelytoformintra-molecularassociation.

简介：Thepurposeofthisstudyistoexploretheexpressionofgrowth-associatedprotein(GAP-43)inspinalcordsegmentsconnectedwithinjuredsciaticnervebythetreatmentwithbrazileininmice.Unilateralsciaticnerveinterruptionandanastomosiswereperformed.Physiologicalsaline(blankgroup),highdose,middledoseandlowdoseofbrazileinwereadministratedintragastricallytohealthyadultBALB/cmiceinseparategroups.L4―6spinalsegmentsconnectedwiththesciaticnervewereharvested.Real-timePCR(Polymerasechainreaction)andWesternblotanalysiswereperformedtodetecttheexpressionofGAP-43inspinalsegments.Histologicalstainingonmyelinandtheelectrophysiologywereperformedtoexaminethesciaticnerverecovery.GAP-43wasactivatedinspinalcordL4―6connectedwithinjuredsciaticnerve.Inthesurvivaltimeof12h,24h,3d,5d,7dand14d,GAP-43expressioninthemotorneuronsofspinalcordofthehighdosegroupandthatinthemiddledosegroupweresignificantlyhigherthanthoseonthelowdoseandblankgroups.MyelininthehighdosegroupandthatinthemiddledosegroupweremorematureandthepotentialamplitudeandMNCV(motornerveconductionvelocity)inthehighandmiddledosegroupswereobviouslyhigherthanthoseinthelowdosegroupandblankgroup.BrazileinfacilitatestheexpressionofGAP-43inneuronsinspinalcordL4―6segmentsconnectedwithinjuredsciaticnerve,whichpromotesnerveregeneration.