简介：从中国福建沿海某一污染滩涂的沉积物中筛选到一株具有较强拮抗水产病原菌的生防细菌LHB02。采用通用引物,对LHB02的16SrRNA片段进行扩增,获得1511bp的DNA系列,基于该DNA系列,结合细菌形态学、生理生化特征,鉴定LHB02为一株枯草芽孢杆菌。对LHB02进行了抗菌试验、抗菌谱检测和发酵条件优化研究,结果表明,LHB02对哈维氏弧菌、溶藻弧菌、鳗弧菌等有很强的拮抗作用,其优化后的发酵条件为：KB培养基（蛋白胨20g,丙三醇10mL,K2HPO41.5g,MgSO4.7H2O1.5g,H2O1000mL）;温度28°C,pH值7.0,培养时间36h,接种量1.5%。

简介：2016年12月,由国家测绘地理信息局局长库热西？买合苏提任主编,国家测绘地理信息局测绘发展研究中心策划的测绘地理信息蓝皮书《测绘地理信息供给侧结构性改革研究报告（2016）》重镑发布.该蓝皮书是国家测绘地理信息局测绘发展研究中心组织编辑出版的第八本测绘地理信息蓝皮书.蓝皮书邀请了测绘地理信息行业的有关领导、专家和企业家撰文,深入分析测绘地理信息行业如何更好地推进供给侧结构性改革.

简介：~~

简介：Astheexplorationandexploitationofoilandgasproliferatethroughoutdeepwaterarea,therequirementsonthereliabilityofdynamicpositioningsystembecomeincreasinglystringent.Thecontrolobjectiveensuringsafetyoperationatdeepwaterwillnotbemetbyasinglecontrollerfordynamicpositioning.Inordertoincreasetheavailabilityandreliabilityofdynamicpositioningcontrolsystem,thetripleredundancyhardwareandsoftwarecontrolarchitecturesweredesignedanddevelopedaccordingtothesafespecificationsofDP-3classificationnotationfordynamicallypositionedshipsandrigs.Thehardwareredundantconfigurationtakestheformoftriple-redundanthotstandbyconfigurationincludingthreeidenticaloperatorstationsandthreereal-timecontrolcomputerswhichconnecteachotherthroughdualnetworks.Thefunctionofmotioncontrolandredundancymanagementofcontrolcomputerswereimplementedbysoftwareonthereal-timeoperatingsystemVxWorks.Thesoftwarerealizationoftaskloosesynchronization,majorityvotingandfaultdetectionwerepresentedindetails.Ahierarchicalsoftwarearchitecturewasplanedduringthedevelopmentofsoftware,consistingofapplicationlayer,real-timelayerandphysicallayer.ThebehavioroftheDP-3dynamicpositioningcontrolsystemwasmodeledbyaMarkovmodeltoanalyzeitsreliability.Theeffectsofvariationinparametersonthereliabilitymeasureswereinvestigated.Thetimedomaindynamicsimulationwascarriedoutonadeepwaterdrillingrigtoprovethefeasibilityoftheproposedcontrolarchitecture.

简介：Outerdensefibersarespecificsubcellularcomponentsofmammalspermflagella,apartfromtheaxonemeanditsassociatedproteins.Asoneoftheouterdensefiberscomponents,ODF3isessentialfortheformationofmammalspermflagella.Inthepresentstudy,weisolatedtheOdf3gene,1033bpinlength,fromZhikongscallopChlamysfarreri,whichwasnamedasCf-Odf3.ItwaspredictedthatCf-Odf3encodesaproteinof240aminoacidswhichcontains5discontinuousPro-Gly-Prorepeats.TheCf-Odf3transcriptsweredetectedinbothtestisandovaryofC.farreriatvariousstagesthroughqRT-PCR.TheCf-ODF3proteinsynthesizedbyprokaryoteswaspurifiedusingNi2+-NTAaffinitychromatographyandusedtoproduceitspolyclonalantibody.TheantibodyproductwaseffectiveandspecificaswasexaminedbyWesternblotting.TheCf-Odf3transcriptandCf-ODF3proteinwerelocatedinintragonadalsomaticcells(ISCs),spermatogoniaandspermatocytesofC.farreritestes,whilenotranscriptionofCf-Odf3andsynthesisofCf-ODF3weredetectedinspermatidsandspermatozoa.ThelocationofOdf3expressionisdifferentfromthatofvertebrateswhereODF3issynthesizedspecificallyinspermatidsandspermflagella.Moreover,theCf-Odf3expressionwasalsodetectedinISCs,oogoniaandoocytesofthescallopovaries.OurfindingrevealedadifferentcharacteristicofOdf3expressionbetweenscallopandvertebrates,whichimpliedthatCf-Odf3playedaroleinthegametogenesisofC.farreri.

简介：EvolutionaryevidencesuggeststhatSox3,amemberofthehigh-mobility-group(HMG)familyoftranscriptionfactors,isanancestralprecursorofSryandisinvolvedinsexdeterminationsimilartoSry.However,thereislimitedinformationregardingtheSOX3geneoftheblackrockfish(Sebastesschlegeli).Inthisstudy,wefirstisolatedSOX3genefromthegonadsofS.schlegelibyhomologycloning.Thefull-lengthofS.schlegeliSOX3(SsSOX3)cDNAwas1386bp,comprisinga906-bpopenreadingframe,whichencodesapeptideshowing93.6%and93.9%homologywiththeSox3proteinsofEpinepheluscoioidesandOryziaslatipe,respectively.ComparisonofthecDNAsequenceoftheSsSOX3genewiththecorrespondinggenomicDNAfragmentrevealedthattheSsSOX3geneconsistsofasingleexon.PhylogeneticanalysisdemonstratedtheevolutionaryrelationshipofSsSOX3withotherknownSOXB1genesinfishandtetrapods.ThepromoterregioncontainsbindingsitesofseveraltranscriptionalfactorsthatmightparticipateintheregulationofSsSOX3expression.Quantitativereal-timePCRanalysisindicatedthatSsSOX3wasexpressedinalltheinvestigatedlarvaldevelopmentalstagesfrom1to35daysafterbirthandthelevelofexpressiongraduallydecreasedasthedevelopmentproceeded.SsSOX3exhibitedsexuallydimorphicexpressioninadultgonads,withhighexpressionintheovarybutlowexpressioninthetestis.InsituhybridizationrevealedthatSsSOX3wasstronglyexpressedinoocytesandfollicularcellsofovariesbutslightlyexpressedingermcellsoftesticulartissues.Therefore,thisstudysuggeststhatSsSOX3playsanimportantroleinoogenesisandovarydifferentiationinS.schlegeli.

简介：Combinedmulti-bodydynamicswithstructuraldynamics,anewdiscreteelementwithflexibleconnector,whichisapplicablefor3-Dbeamstructures,isdevelopedinthispaper.Boththegeneralizedelasticcoefficientmatrixoftheflexibleconnectorandthemassmatrixofdiscreteelementmaybeoff-diag-onalinageneralcase.Thezero-lengthrigidelementisintroducedtosimulatethenodeatwhichmultipleel-ementsarejointedtogether.Itmayalsobeeffectivewhentheaxesofadjacentelementsarenotinthesameline.Theexamplesforeigenvaluecalculationshowthatthemodelissuccessful.Itcanbeextendedtothegeometricnonlinearresponseanalysis.

简介：AbundantevidencesofhighersealevelsfromJiangsuandFujiancoastshaveprovedamarinetransgressioneventduring30–40kaBP,suggestingthattherewasastagewithhighsealevelandawarmclimatewhenicesheetsshrankintheNorthernHemisphere.Thedurationof30–40kaBPspannedaperiodinthelateMarineIsotopeStage3(MIS3)andwasinnatureaninterstadialepochduringtheLastGlacialperiodoftheQuaternary.Differentfromtheglacialperiodwithacoldclimate,thismarinetransgressionconsideredasapenultimatehighersealevelduringtheQuaternaryremainsapuzzlethatwhytheevidenceiscontrarytotheQuaternaryglacialtheory.Itisimportanttounderstandsealevelrisefortheseareassensitivelyrespondingtotheglobalchangesinthefuture.Torecognizethekeyissuesonsealevelchanges,theeustaticsealevel(H_S)wasdefinedastheglaciation-climateforcedsealevels,andtherelativesealevelchange(H_R)wasdefinedasthatasealevelrecordwaspreservedinsedimentthatexperiencedmultiplesecondaryactionsoflandandseaeffects.Onthebasisasdefinedabove,weconstructedmulti-levelmodelsofclimate-drivenglacio-eustaticchangesandland-seasystems.Byintegratingdatasetsfromeightboreholecoresandprescribingtheboundaryconditions,wesimulatedthechangesofHSandHRintheEastChinaSeaandsouthernYellowSeaareasinthelateMIS3.Themarinetransgressionstratafromtheboreholecoredatawasidentifiedatca.30mbelowpresentsealevelasaresultofthecollectiveinfluenceoficemeltingwater,neotectonicsubsidence,sedimentcompactionandterrestrialsedimentfillingsinceca.35kaago,whereasthesimulatedrelativesea-levelsturnedouttobe–26.3––29.9ma.s.l.Thesmallerrorinvolvedinthesimulationresultsof±(2.5–4.5)mdemonstratedthecredibilityoftheresults.OurresultsindicatedthatsealevelchangeinthelateMIS3wasdominatedbyglacialeffects,inwhichtheeustaticsea-levelwasbetween–19.2�

简介：在视口数据检索之后,视口重建效率成为了影响地形数据三维重建和显示速度的主要因素,视口重建与可利用的开发平台资源密切相关.在剖析Direct3D硬件访问方式的基础上,提出了一套完整的DEM快速重建方法.实验结果表明在相同的条件下,基于Direct3D的视口重建具有明显的速度优势.

简介：FattyacidcompositionsoftwostrainsofmarinediatomsNitzschiaclosterium(MACCB222)andChaetocerosgracilis(MACCB13)havebeenexamined.ThemicroalgaehavebeengrownatdifferentinitialNaNO3concentrations(75,375,750,1125,1500,1875mg/L)andharvestedatthelateexponentialphase.TheresultsbyonefactoranalysisofvarianceshowthattheNaNO3concentrationshavenosignificantinfluenceontherelativegrowthrateoftwostrainsofmarinediatoms;theinfluenceofNaNO3concentrationsonthefattyacidsdiffersfromspeciestospecies.Themajorfattyacidsoftwodiatomstrainsare14:0,16:0,16:ln-7and20:5n-3(EPA),B13alsohashighpercentageof20:4n-6(6.0～9.1%).

简介：有不同规模的很多海底的山崩在Liwan3-1气体地的海底的管道线路的峡谷区域被识别了。仍然有许多机会，海底的斜坡失败将发生，并且下列集体运动将介绍大风险给海底的管道。鉴于这，一个数字预言方法基于Eulerian-Eulerian二阶段的流动模型被介绍模仿潜在的海底的山崩的集体运动。滑动土壤和周围的水被Herschel-Bulkley流变学模型和牛顿的液体模型分别地模仿。骚乱用k-模型被模仿。与两个相比实验数据并且是结果，二阶段的流动模型显示出好精确性，并且它的结果是更多关门到实际状况；在土壤和周围的水之间的动态联合能有效地被模仿，hydroplaning和头分开的现象能被获得。最后，潜在的海底的山崩的土壤运动作为一个例子被模仿，根据在峡谷区域的地震侧面。结果证明hydroplaning发生在运动过程期间。二阶段的流动模型计算的刺杀距离是877m，它是27.1%比是大结果。然而，土壤的山峰前面速度是相对的小，与8.32m/s的最大的价值。是有一个简单、快速的过程的节目能被用于初步的评估，当二阶段的流动模型为一个精确评价是更适当的时。

简介：这篇论文为基于的自我类似论述一个便宜方法由使用高度和反照率地图真实世界的3D表面质地编辑。编辑的基于的2Dtexture接近仅仅做变化到象素颜色或紧张的不同自我类似珍视，这种技术也允许表面几何学和捕获的3D表面质地的反射到用原版不同于那些的照明条件和视场角是ited和relit。在一个给定的地点的单个编辑操作影响所有类似的区域并且在在不同条件下面显示的样品的所有图象上生产变化。后来，表面高度和反照率地图能被用来描述海床地形学和地质的特征，它在许多海洋的进程起重要作用，建议方法能有效地关于海洋的现象的可视化和模拟在应用程序被采用。

简介：Atime-domainmethodisappliedtosimulatenonlinearwavediffractionaroundasurfacepiercing3-Darbitrarybody.ThemethodinvolvestheapplicationofTaylorseriesexpansionsandtheuseofperturbationproceduretoestablishthecorrespondingboundaryvalueproblemswithrespecttoatime-independentfluiddomain.AboundaryelementmethodbasedonB-splineexpansionisusedtocalculatethewavefieldateachtimestep,andthefreesurfaceboundaryconditionissatisfiedtothesecondorderofwavesteepnessbyanumericalintegrationintime.Anartificialdampinglayerisadoptedonthefreesurfacefortheremovalofwavereflectionfromtheouterboundary.Asanillustration,themethodisusedtocomputethesecond-orderwaveforcesandrun-uponasurface-piercingcircularcylinder.Thepresentmethodisfoundtobeaccurate,computationallyefficient,andnumericallystable.

简介：Thetyphoon,asamaturetropicalcyclonethatdevelopsinthewesternpartoftheNorthPacificOceanwithhighwindspeedandheavyrainfall,isoneofthemostlethalandcostlyofnaturaldisastersforthedenselypopulatedcountriesofEastAsia.Itcanbeeasilydetectedbyspace-bornesensorsoperatedatmicrowave,visibleorinfraredbands(Liuetal.,2014).SyntheticApertureRadar(SAR)is

简介：在本研究中，我们首次通过基因组上游第一次步移克隆到了雨生红球藻中crtO基因的3个不同大小的5’上游侧翼序列1．1kb，1．9kb和2．2kb。利用生物信息学方法分别预测并比较了这3个不同大小的5’上游侧翼序列，结果发现，三者具有与高等植物相类似的顺式作用元件，如脱落酸反应元件（ABRE）、干燥或低温反应元件（DRE／C-repeat）、几种光反应元件（G1-box,GAG-motif，l-boxandATC—motif）、热激反应元件（HSE）、机械伤害反应元件（WUN-motif）、生长素反应元件（TGA-element）、茉莉酸甲酯反应元件（TGACG-element）和反式作用因子MYB蛋白的结合位点（MBSandMRE）等等，但是三者并不具有典型的TATA框和CCAAT框。上述研究预示了雨生红球藻虾青素合成中crtO基因调控方式的多样化。

简介：基于广州及深圳海岛海岸带区域的1：2000航空摄影测量项目,对比分析A3航摄仪在有、无地面像控点情况下的空中三角测量精度.结果表明,在无地面像控点的情况下,A3仍可满足海岛海岸带区域大比例尺成图的精度要求,且航摄效率高、成图周期短.本研究可为A3在海岛海岸带区域的应用提供参考.

简介：在本研究中,我们首次通过基因组上游第一次步移克隆到了雨生红球藻中crtO基因的3个不同大小的5’上游侧翼序列1.1kb,1.9kb和2.2kb。利用生物信息学方法分别预测并比较了这3个不同大小的5’上游侧翼序列,结果发现,三者具有与高等植物相类似的顺式作用元件,如脱落酸反应元件(ABRE)、干燥或低温反应元件(DRE/C-repeat)、几种光反应元件(G-box,GAG-motif,I-boxandATC-motif)、热激反应元件(HSE)、机械伤害反应元件(WUN-motif)、生长素反应元件(TGA-element)、茉莉酸甲酯反应元件(TGACG-element)和反式作用因子MYB蛋白的结合位点(MBSandMRE)等等,但是三者并不具有典型的TATA框和CCAAT框。上述研究预示了雨生红球藻虾青素合成中crtO基因调控方式的多样化。更多还原

简介：柱体的垂直摆动与不同直径比率Dd/Dc和厚度比率td/Dc被3D学习(三维)在这份报纸开发的数字模型，并且通过2D与结果相比获得了(二维)数字模型。高顺序的迎风的计划被使用稳定计算，并且集中被多格子方法加速。2D和3D模拟结果之间的差别的质、量的分析与一个依附底部的磁盘在流动地特征和垂直地震荡的柱体的水动力学系数上揭示3D效果。液体领域上的3D效果主要在三个流旋涡的模式的意义被反映：x相对在2D模拟产生的旋涡在锋利的边附近在流动地上有更大的效果。在沿着轴的取向的片，沿着光线的轴的y比沿着环绕的方向的x，更在速度上显示光线的效果在磁盘的锋利的边附近比环绕的效果读了。旋转相互作用在水平飞机的液体的z在举运动期间是不足道的。基于2D和3D模拟结果，分开增加的集体系数和抑制比率的增加的政体的转弯的点被识别。直径比率Dd/Dc和厚度比率td/Dc详细被讨论。

简介：Inthisstudy,threecDNAsequencescorrespondingtocytochromeP450C17(CYP17I),3β-hydroxysteroiddehydrogenase(3β-HSD)andandrogenreceptor(AR)wereisolatedfromspottedseabass(Lateolabraxmaculatus).ThemRNAabundancesofCYP17Iand3β-HSDincreasedfromstageⅡtostageⅤwithasignificantincreaseatstageⅤ,andthehighestabundanceofARmRNAwasdetectedatstageⅢintesticulardevelopmentcycle.CYP17I,3β-HSDandARtranscriptswereobviouslyabundantinsteroidogenesistissuessuchastestis,brain,headkidneyamongothers.StrongandpositivesignalswereobservedmainlyininterstitialcellregionsofL.maculatustestisasweremeasuredwithinsituhybridizationmethod.SignificantincreasesofCYP17Iand3β-HSDtranscriptsweredetectedafter12–48hhCG(humanchorionicgonadotropin)andGnRHa(gonadotropin-releasinghormoneanalogue)treatments.However,anoppositerelationshipwasfoundforARintestisatthesametime.Inaddition,decreasingtrendsofCYP17Iand3β-HSDmRNAwereobservedintestisofL.maculatusinfreshwatergroup(FW)fromday2today6,andmRNAabundanceofARincreasedinbrackishwater(BW)groupfromday4today8.Thesefindingsrevealedthatthesethreesteroidsynthesisgenesareimportfortesticulardevelopment,hormoneandsalinitytreatment,andprovidedalsoaninsightintothemechanismofreproductiveendocrineofL.maculatus.