简介：Fingeringphenomenaarecommonoccurrenceinthenaturalworld.Itgenerallytakesplacewhenalessviscousfluiddisplacesamoreviscousfluidtypicallyinporousmedia.Nowadays,suchphenomenahaveextensivelybeenstudiedduetoitsimportanceinmanyindustrialfields.Inthispaper,theeffectsofsurfacewettabilityonfingerpatternarestudiedandsimulatednumericallybytheLatticeBoltzmannmethod(LBM).Thedisplacementefficiencyisinvestigatedbyusingtwoparameters,namely,thebreakthroughtimeandthearealsweepefficiency.Thesimulationhasdemonstratedthatsurfacewettabilitywillinfluencethefingerpatternnomatterthegravityisconsideredornot,butinthepresenceofgravity,thefingerpatternismuchmorecomplicatedandirregularduetothecoexistenceandcompetitionofcapillaryforce,viscousforceandgravity.

简介：Wedescribeanoveltechnique,lowsurfaceenergyGasExpansionMolding(GEM),tofabricatemicrobubblearraysinpolydimethylsiloxane(PDMS)whichareincorporatedintoparallelplateflowchambersandtestedincellsortingandmicrocellcultureapplications.Thisarchitectureconfersseveraloperationaladvantagesthatdistinguishthistechnologyapproachfromcurrentlyusedmethods.HereinwedescribetheGEMprocessandtheparametersthatareusedtocontrolmicrobubbleformationandaVacuum-AssistedCoating(VAC)processdevelopedtoselectivelyandspatiallyalterthePDMSsurfacechemistryinthewellsandonthemicrochannelsurface.WedescriberesultsfrommicroflowimagevisualizationstudiesconductedtoinvestigatefluidstreamsaboveandwithinmicrobubblewellsandconcludewithadiscussionofcellculturestudiesinPDMS.

简介：AsapartofabasicresearchprojectonXeno-transplantion,wehavebeenengagedinthederivationofembryonicstemcelllinesfromChineseminiswine.Here,wereportedforthefirsttimetheestablishmentoftwoporcineEGcelllines(BPEG1andBPEG2)fromprimordialgermcellsofgenitalridgesofa28anda27dembryosrespectively.Theirpluripotentnaturehasbeenidentifiedbycolonymorphology,markercharacterizationaswellasbyinvitroandinvivodifferentiation.TheseporcineEGcellsarepotentiallyusefulforfurtherbasicstudies.

简介：我们以前证明Wnt3a能刺激人的胚胎的茎(hES)细胞增殖并且影响房间命运决心。当feedercell-derivedfactors不在时，在一个喂入器饲料分送器免费的条件下面有教养的hES房间糟糕幸存并且增殖。当feeder房间不在时的AddingrecombinantWnt3a导出因素的刺激hES细胞增殖而且区别。在现在的学习，我们进一步扩大了我们的分析到象Wnt1和Wnt5a那样的另外的Wntligands。当Wnt1作为Wnt3a在hES房间上显示了类似的效果时，在这个系统的Wnt5ahad小效果。当喂入器饲料分送器导出自强因素和bFGF也是现在时，Wnt3a和Wnt1提高了无差别的hES房间的增长。探索可能性由激活Wnt发信号支持无差别的hES细胞的增长，在使不朽的人的成年成纤维细胞(HAFi)的weoverexpressedWnt3a或Wnt1基因在比主要老鼠支持无差别的hES细胞的长期的生长是优异的细胞胚胎的成纤维细胞。HAFi房间与或没有Wnttransgene能被宣传Wnt3a基因的indefinitely.Over表示显著地提高了HAFi喂入器饲料分送器房间的能力支持我们测试了的3根不同hES房间线的无差别的生长。在Wnt3a-overpressingHAFi房间的threecommonly-used药选择基因的合作表示进一步使我们能在稳定的transfection或转导以后选择稀罕hES克隆。这些使不朽的设计喂入器饲料分送器房间(W3R)那列合作快车象Wnt3a那样的支持生长的基因和三药选择基因应该授权我们高效地为基本、翻译的研究使修改hES房间线基因。

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