Hereinwedemonstratetheconstructionofthreetypesofparallelgoldnanorod(AuNR)clustersusingaDNAorigamirod(DOR)asthetemplate.BasedontheprecisecontroloverthepositionofcapturestrandsonDOR,numberandorientationoftheAuNRclusterscanbewellengineered,asevidencedbybiologicaltransmissionelectronmicroscope(TEM).Importantly,theAuNRclustersexhibitchiropticalresponseswhicharestronglyaffectedbythenumberofAuNRonrod-likeDNAorigami.