Proteins binding to the 5‘—flanking regualtory elements of the human β—globin gene

(整期优先)网络出版时间:1993-02-12
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Thebindingofnuclearproteinspreparedfrommouseerythroidtissueindifferentdevelopmentalstagestothe5'-flankingregulatoryelementsofhumanβ-globingene,twonegativecontrolregions(NCR1,-610to-490bp;NCR2,-338,to-233bp),wasidentified.TwostagespecificproteinfactorscorrespondingtoembryonicandfetalstageswerefoundtobecapableofbindingtoNCR2.Thesedataprovidedevidencethatthecisactingelementsofthe5'-flankingregionmightbeinvolvedinthedevelopmentalcontrolofβ-globingeneandNCR2mightberesponsibleinartforthesilenceofβ-glolbingeneintheembryonicandfetalstages.