Soilswerecollectedfrom2-year(2-y)and3-year(3-y)oldred-pineseedlingplotsintwotreenurseries,HaywardinthenorthandWilsoninthesouthwesternpartofWisconsinStaterespectively,andequilibratedwith0.01MCa(NO3)2)forsoilsolutionZnandMn(solu-ZnandMn),andwith0.01MCa(NO3)2+0.005MEDTAforsoilabsorbedZnandMn(ad-ZnandMn).BufferingcapacityofsoilZnandMn(b-ZnandMn)wasobtainedfromtheratioofad-ZnandMntothesolu-ZnandMn.Theconcernedtracesinpineseedlingneedles(ndls),stems(sts)androots(rts)weresimultaneouslymeasured.Theresultsobtainedshowthat:About60%ofsolu-andad-Znrangedfrom0.2to0.4andfrom1to2μg/gsoilrespectively.About70%ofb-Znwaswithin3-10.Thehighestcontentofsolu-Zncomparedwiththelowestshowedadiscrepanceofmorethan10-fold.ThetwoformsofsoilZnwerecommonlyhigherinWilsonthaninHaywarkNursery.About80%ofsolu-,ad-andb-Mnwerewithin3-10,5-5.8μg/gsoiland1-2respectively.Influenceoflowbufferingcapacityonsolu-ZnandMnwasabout20timesstrongerthanthatofthehigh.TheE-value,aratioofaccumulatedZnandMninneedlestothoseinthesoilsolution,isprovedtobe:E-Zn>E-Mn;E-sts>E-ndlsorE-rts;andE-2y>E-3y.Curvilinearand/orlinearcorrelationsbetweensoilsolu-,ad-andb-ZnandMnandndls-,sts-,rts-ZnandMnwereatverysignificantorsignificantlevels.Forpredictingndls-ZnandMn,tworealizableandsimplemodelsfromtworegressionequationswereestablishedthroughtheselectionofrelatedparametersanddependentvariables.BinaryregressionanalysisbasicallyeliminatedtheinfluenceofsoilpHonthepredictionofZnandMninneedles.SoilpHwasthusthoughttobeexcludedfromthemodel.