Inordertoimprovethestabilityandcorrosioninhibitionperformanceofbioenzyme,lipaseandlsozymewereco-immobilizedonthemesoporousmolecularsieveMCM-41bytheadsorptionmethod.Thentheimmobilizedenzymeswerecombinedwithaminotrimethylenephosphonicacidandpolyasparticacidtopreventcorrosioncausedbycirculatingcoolingwater.Theweight-lossmethodandelectrochemicaltechniqueswereusedtoevaluatetheperformanceofcompositeinhibitors.Theco-immobilizedlysozymeandlipaseachievedgoodinhibitioneffects.Aftertheywerecombinedwithaminotrimethylenephosphonicacidandpolyasparticacid,thecorrosioninhibitionpropertieswerefurtherimproved.Theinhibitionefficiencywaspromotedto94.4%.Duringthecorrosioninhibitionprocess,theimmobilizedenzymesplayedanimportantrole.Theadditionofcorrosioninhibitorcouldinhibittheanodicdissolutionandcathodichydrogenevolutionprocessofcarbonsteelatthesametime.Theadsorptionofco-immobilizedlysozymeandlipasecompositeinhibitoronthesteelsurfacewasajointactioninvolvingphysicaladsorptionandchemicaladsorption.