Cytogenetic analysis in two scallops (Bivalvia: Pectinidae) by PRINS and PI banding

(整期优先)网络出版时间:2007-05-15
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Cytogeneticanalysiswasperformedforthebayscallop(ArgopectenirradiansLamarck1819)andtheJapanesescallop(PatinopectenyessoensisJay1857)byprimedinsitulabeling(PRINS)andpropidiumiodide(PI)bandingtechniques.ThePRINSanalysisrevealedthatmajorrRNAgeneswereclusteredintwolocionthetelomericregionsoftheshortarmsontwoacrocentricchromosomepairsinA.irradiansandontwosubmetacentricpairsinP.yessoensis.ThehistoneH3genesitesdifferedinnumberandlocationbetweenthesetwospecies.TheC-band-likepatternsrevealedbyPIstainingvariedconsiderablybetweenthesetwospecies.A.irradiansdisplayedterminalbandsatlongarmsonallchromosomes,centromericbandsonsomepairsandinterstitialbandsonfivepairs.P.yessoensisexhibitedonlycentromericbandsonallchromosomes.TheseresultswouldcontributetothebetterunderstandingofkaryotypeevolutioninA.irradiansandP.yessoensis.