简介：Theenhancedcardiaccontractilityeffectofhumanrecombinantgrowthhormone(hr-GH)onthecongestiveheartfailure(CHF)wasstudiedonthepig.Tobuildapigmodelofcongestiveheartfailure,atemporaryartificialcardiacpacemakerwasimplantedinthepig'sbodyandpacedat220beatsto240beatsperminutefor1week.Afterthemodelofcongestiveheartfailurewassuccessfullysetup,thefrequencyofthepacemakerwaschangedto150beatsto180beatsperminutetomaintaintheCHFmodelstable.Pigsweredividedintothreegroups:Thehr-GHgroupinwhich0.5mg/kgperdayofhr-GHwasadministratedintramuscularlyfor15days,theinjectioncontrolgroupinwhichanequalamountofphysiologicalsalinewasinjectedintramuscularly,andanormalcontrolgroup.Theleftventriculardiastolicendpressurewas(10.60±2.41)mmHginthehr-GHgroup,but(19.00±3.81)mmHginthesalinecontrolgroup(P＜0.01);Cardiacoutputwas(1.86±0.13)L/mininthehr-GHgroup,but(1.56±0.18)L/mininthesalinecontrolgroup(P＜0.05);Peripheralmin)-1inthesalinecontrolgroup(P＜0.05);±dp/dtmaxwas(2900±316.23)and(2280±286.36)inthehr-HGgroupandthesalinecontrolgrouprespectively(P＜0.05).Theresultsshowthathr-GHenhancesmyocardialcontractilityofCHF,andtheCHFmodelbuiltbyatemporaryartificialcardiacpacemakeratahighrateofstimulationisreasonableandapplicable.

简介：本文首先介绍了固定CO2的意义及方法,然后分析了微生物固定CO2技术流程,接下来阐述了微生物种群及生物反应器类型,最后重点讨论了微生物固定CO2的应用(以微型藻类在CO2吸收与资源化中的应用为例),以期为同行提供有益借鉴与参考。

简介：选择60Coγ射线5Gy照射后第7天的人胚肺成纤维细胞(HELF),采用聚阳离子脂质体LipofectAMINE介导的基因转染法将人TGFβ1正、反义基因表达载体pMAMneo-TGFβ1和pMAMneo-AntiTGFβ1转入HELF,转染细胞经G418抗性筛选出来.提取培养细胞的染色体DNA,用DNA斑点印迹分析表明,它们能与neo基因特异杂交.用neo基因特异的PCR引物能从转染细胞扩增出276bp的阳性片段,而未转染细胞则扩增阴性.