简介：Viaseveraltechniquestomodifysensitivematrixforinhabitingtheleakageoffluorescentindicator,anewstablesensingmaterialformonitoringphosphateshasbeenpreparedandappliedtothemeasurementofphosphatesinartificialseawater.ItisbasedonthereactionofPO3-4withAl(Ⅲ)-Morinthatleadstothefluorescencequenchingofthecompositematrix.AtpHvalue4.0andthesalinityvalue25.0,theresponsetimeofoutputsignalhavingreachedthesteadystateislessthan300seconds.ItscalibrationgraphisgainedintherangeofH2PO-4massconcentrationfrom1.50to7.00μg/mLwiththelimitofdetection(3σ/K)0.02μg/mL.Whenitwasusedtomeasurephosphatesinartificialseawater,therecoveryrangedfrom96.78%to102.09%.Beingstoredunderthepropercondition,themembranesmaintainsensitivefor90-120days,andareabletobeusedfor50-80timeswithindicatorsupplement.

简介：新benzoimidazole-naphthalimide衍生物4被综合，它的photophysical性质被学习。这混合物有选择地高度出现了并且敏感比色并且为氟化物阴离子的ratiometric察觉到能力。

简介：ThesynthesisofanewtypeofX-ray-sensitivecompound'di-(1-hydroxylundecyl)diselenide'anditsapplicationinthepreparationofanewtypeofliposomewithX-raysensitivitywasreported.Thisnewliposomewassynthesizedtoencapsulatedoxorubicinhydrochloride(Dox),withitsphysicalandchemicalproperties,stability,andradiationsensitivitydetermined.BasedonthepH-gradientmethod,liposomalDoxwaspreparedviaultrasonicemulsificationandthenpurifiedonaSephadexG50mini-column.UVspectrophotometryandliquidchromatographywereusedtodetecttheencapsulationefficiencyandradiationsensitivityoftheDox-loadedliposome.Theresultsshowthatthroughchangesinreleaserate,thisliposomeshowsarelativeradiosensitivity.Intermsofradiationsensitivity,thedrugleakrateoftheX-ray-sensitiveDox-loadedliposomeincreasedgraduallyandpeakedat65.4%undertheX-rayradiationofadoseof10Gyormorethan10Gy,whichissignificantlydifferentfromthatofordinaryliposomes.Meanwhile,X-ray-sensitiveDox-loadedliposomehasagooddispersionstability,withanaverageparticlesizeofapproximate120nm.TheefficiencyofthisliposomeencapsulatingDoxwas75.84%,slightlylowerthanthatofordinaryliposomes.TheX-ray-sensitiveDox-loadedliposomeexhibitedsuspensionstabilitywithin30dofstorageat4°C,withoutvisibleprecipitation.Di-(1-hydroxylundecyl)diselenideissafeandnoncytotoxicandcomparedwiththoseofsyntheticphospholipidsitssynthesisislowcostanddoesnotrequirecomplexconditions.

简介：CunanoclusterswereelectrochemicallydepositedonthefilmofaNafion-solubilizedmulti-wallcarbonnanotubes(CNTs)modifiedglassycarbonelectrode(CNTs-GCE),whichfabricatedaCu-CNTscompositesensor(Cu-CNTs-GCE)todetectglucosewithnon-enzyme.Thelinearrangeis7.0×10-7to3.5×10-3mol/Lwithahighsensitivityof17.76μA/(mmolL),withalowdetectionlimit2.1×10-7mol/L,fastresponsetime(within5s),goodreproducibilityandstability.

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简介：Inthispaper,itwasdiscoveredthatanovelpH-sensitivecopolymerofN-isopropylacrylamide(NIP)andN-(3-dimethylaminopropyl)methacrylamide(DMAPM)couldbegottenbypolymerization.ThephasetransitionpH(pHtr)ofP(NIP-DMAPM)polymerwasfoundtobe7.4at37℃.ThepolymerwasprecipitatedoutofwateraboveacriticalpH=7.4andre-dissolvedbelowpH----7.4.Thecharacteristicofthispolymermadeitpossibletocarryouttheimmunochemicalstepsofanimmunoassayinatruesolutionandthentoquicklyseparatetheresultingproductfromthereactionmixture.Inacompetitivefluorescenceimmunoassay,thestandardrabbitIgGandrabbitIgGimmobilizedonP(NIP-DMAPM)firstcompetitivelyreactedwiththefluoresceinisothiocyanate(FITC)labeledantibody,thenthepHofsolutionwasadjustedabovethepHtrofpolymertoprecipitatethepolymer-immunecomplex,andthepolymer-immunecomplexprecipitatewasseparatedandre-dissolvedbytheadjustmentofpH,finallytheFITC-labeledantibodyintheimmunecomplexwasquantifiedbyfluorescencemeasurement.ThecalibrationgraphforrabbitIgGwaslinearovertherangeof100-1000ng/mLwithadetectionlimitof11ng/mL.Themethodisrapid,sensitiveandsimple.OwingtoneutralpHtrofP(NIP-DMAPM),thedamagetoantigen-antibodyimmunecomplexwasgreatlydecreasedinthecourseofseparation.Inaddition,asandwichenzyme-linkedfluorescenceimmunoassaymethodforthedeterminationofhumanIgGwasalsodeveloped,showingthatthepH-sensitivephaseseparatingimmunoassaycouldbeperformedinthecompetitivemethodaswellasthesandwichmethod.

简介：Graphenenanosheets(GS)wereeasilypreparedthroughliquid-phaseexfoliationofgraphitepowderinN,N-dimethylformamide(DMF)withtheassistanceofsodiumcitrate.Then,GSwascoatedontoaglassycarbonelectrode(GCE)surfacebydroptofabricateaGS/GCEnanointerface.Subsequently,byusingtetraethylorthosilicatesolasprecursor,nanosilicawaselectrochemicallydepositedontotheGS/GCEsurfacetoproduceananocompositefilmelectrode(nanosilica/GS/GCE).Electrochemicalbehaviorsofmethylparathion(MP)onthenanosilica/GS/GCEsurfacewereinvestigatedthoroughly.Itwasfoundthatthenanosilica/GSnanocompositescanimprovetheredoxpeakcurrentsofMPsignificantlyduetothesynergeticeffect.TheoxidationpeakcurrentwaslinearlyrelatedtoMPconcentrationintherangefrom0.0005mmol/Lto5.6mmol/L.Thedetectionlimitwascalculatedtobe0.07nmol/L(S/N=3).ThedevelopedmethodwasusedtodetermineMPinrealsamples.Therecoverieswereintherangefrom95.4%to104.2%,demonstratingsatisfactoryresults.

简介：Micelles,microemulsions.vesicles,etc.,areorganicmicroheterogeneousmedia.Useofthesemediainanalyticalreactionsfordevelopingnewkinetic-baseddeterminationsorimprovingpreviouslyestablishedkineticmethodshasbeenreported'2.Inrecentyears,amixedmicellarmediumc...

简介：Ahighlysensitiveelectrochemiluminescence-polymerasechainreaction(ECL-PCR)methodforK-raspointmutationdetectionisdeveloped.Briefly,K-rasoncogenewasamplifiedbyaRu(bpy)32+(TBR)-labeledforwardandabiotin-labeledreverseprimer,andfollowedbydigestionwithMvaIrestrictionenzyme,whichonlycutthewild-typeampliconcontainingitscuttingsite.Thedigestedproductwasthenadsorbedtothestreptavidin-coatedmicrobeadthroughthebiotinlabelanddetectedbyECLassay.TheexperimentresultsshowedthatthedifferentgenotypescanbeclearlydiscriminatedbyECL-PCRmethod.Itisusefulinpointmutationdetection,duetoitssensitivity,safety,andsimplicity.

简介：这份报告描述了能够基于调停toehold的海滨排水量反应(toehold-SDR)和microbead俘获技术的联合在正常温度检测单个核苷酸多型性的一个免费酶、方便、便宜的genotyping生物传感器。生物传感器由一根记者探针和一根俘获探针形成的pre-hybridized海滨组成。面对一个变异的序列，没有调停toehold的海滨排水量，记者探查不能被免除pre-hybridized海滨。Microbeads俘获荧光灯通过biotin-streptavidin相互作用的pre-hybridized海滨microbeads给出重要荧光信号，当在答案没有荧光时。面对一个匹配的目标，然而，海滨排水量有效地被开始，记者探查被免除pre-hybridized海滨。在增加microbeads以后，解决方案生产明亮的荧光，当时microbeads没有明显的信号。遗传型根据答案的荧光紧张方便地被识别。方法提供简单、便宜的策略检测点变化。而且，这个生物传感器在1-40 的范围显示出线性关系；nmol/L和活动范围0.3 的察觉限制；nmol/L。

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