简介：SulfatecanbeactivatedbyATPsulfurylaseandadenosine5’-phosphosulfatekinase(APSK)invivo.RecentstudiessuggestedthatAPSKinArabidopsisthalianaregulatedthepartitionbetweenAPSreductionandphosphorylationanditsactivitycanbemodulatedbycellularredoxstatus.InordertostudyregulationofAPSKinrice(OsAPSK),OsAPSK1genewasclonedanditsactivitywasanalyzed.OsAPSK1C36andC69werefoundtobetheconservedcounterpartsofC86andC119,whichinvolvedindisulfideformationinAtAPSK.C36A/C69AOsAPSK1doublemutationwasmadebysitedirectedmutagenesis.OsAPSK1anditsmutantwereprokaryoticallyover-expressedandpurified,andthenassayedforAPSphosphorylationactivity.OsAPSK1activitywasdepressedbyoxidizedglutathione,whiletheactivityofitsmutantwasnot.Furtherstudiesinthecasethatoxidativestresswillfluctuateinvivo3’-phosphoadenosine-5’-phosphosulfatecontent,andallAPSKisoenzymeshavesimilarregulationpatternsarenecessarytobeperformed.

简介：Twostarch-branchingenzyme(SBE)inrice,isknowntobeakeyenzymeinamylopectinbiosynthesis.ThecDNAoftwoSBE(starch-branchingenzyme)genesSheIandShedencodingSBEⅠandSBEⅢ(twomajorisoformsinrice)wereclonedbyanimprovedRT-PCRtechnique,fromatemplatecDNAlibray,derivedfromthetotalmRNAsextractedfromtheimmatureseedsofajaponicariceWuyunjing7.DNAsequenceanalysisshowedthatthesizeoftheclonedSheIandShedcDNAswere2490and2481bplong,respectively,includingtheirentirecodingsequences.ComparisonanalysisindicatedthatthenucleotidesequenceofShe3wasthesameasthatofshed(GenbankAccessionNo.D16201)asreportedpreviously.Therewereonlyfourbase-pairsdifference,whichresultedinchangesoftwodeducedaminoacidsbetweentheclonedShe1cDNAandthereportedshe1(GenbankAccessionNo.D11082).TheclonedSheIandShedcDNAsmakeitpossibletoimprovericestarchqualitythroughgeneticengineering.

简介：Lipoxygenase3(LOX3)isamajorcomponentoftheLOXisozymesinmaturericeseeds.ToinvestigatetheroleofLOX3geneunderstresses,aplantexpressionvectorcontainingantisensecDNAofLOX3wasconstructed.RicevarietiesWuyunjing7andKasalathweretransformedbytheAgrobacterium-mediatedmethodandtransgenicriceplantsweregenerated.PCRandSouthernblotresultsshowedthattheantisenseLOX3genewasintegratedintothericegenome.AnalysesofembryoLOX3deletionandsemi-quantitativeRT-PCRconfirmedtheantisensesuppressionofLOX3geneintransgenicplants.TheT2antisenseplantsofLOX3weresensitivetodroughtstress,riceblastandbacterialblightcomparedwithnon-transgenicplants.TheseresultssuggestthattheLOX3genemightfunctioninresponsetostresses.

简介：TounderstandthewildOryzagenomeeffectonphotosynthesisanditsrelationtototaldrymatteraccumulationinanelitericevariety,asetof40stableintrogressionlines(ILs)BC3F8derivedfromacrossofOryzasativa(KMR3)×Oryzarufipogon(WR120)weregrownunderwellwateredconditions.Leafgasexchangemeasurementsandleafchlorophyllestimateswereconductedatthefloweringstage.Theresultsrevealedsignificantvariationsinnetphotosyntheticrate(Pn),transpirationrate(E),transpirationefficiency(Pn/E)andcarboxylationefficiency(Pn/Ci).PnshowedsignificantpositivecorrelationwithE,stomatalconductance(gs),Pn/Ciandtotalcanopydrymatter.SpecificleafareaandleafthicknesswerenotsignificantlycorrelatedwithPn.Thirty-sevenoutof40ILsshowedhigherPnthanKMR3[11.28μmol/(m2·s)],and20ILsshowedhigherPnthanWR120[15.08μmol/(m2·s)].ThelineIL194showedthehighestPn[21.62μmol/(m2·s)]withincreasedtotalcanopydrymatterfollowedbylinesIL381,IL106,IL363-12,IL198,IL86-18andIL50,whichexhibitedPnabove18.0μmol/(m2·s).TheILswithenhancedPnareapotentialsourcefordevelopingricevarietiesandhybridswithhigherbiomassandyield.

简介：TheinteractionbetweenricehostanditspathogenXanthomonasoryzaepv.oryzae(Xoo)atcellularlevelwasstudiedbyusingaresistantsomaclonalmutantHX-3anditssusceptabledonorMinghui63.AfterinoculationwithXoostrainZhe173(Chinesepathotype|\).theactivityofsuperoxidedismutase(SOD)andperoxidase(POD)inthecallusofMinghui63wasincreaseddramatically,andtheactiveoxygen(O2^-)wasproducedatahigherrate;Meanwhile,thecallusgrewslowlywiththereductionofproteincontent.ComparedtotheactivityofSODandPOD.theproductionrateofO2^-andthefreshweightinHX-3callusvariedlittleaftertheinoculation.ItcouldbeproposedthatthereweregreatdifferencesbetweentheresistanceofHX-3andMighui63atcellularlevel.TherewasnodifferencedetectedconcerningresistancetobacterialleafblightinHX-3betweentheplantandthecallus.

简介：到精选精英germplasms，从装饰用的梨树米饭栽培变种Wuyujing3导出的112异种被评估。象圆锥花序那样的收益部件每平方米数，谷物数字每圆锥花序，和谷物，重量被测量。象白垩的谷物(PCG)的百分比那样的优秀特点，稻谷收益(BRY)，milled米饭收益(MRY)，milling(DM)的度，直链淀粉内容(交流)，蛋白质内容(PC)，和在特点之中的关系是inverstigated。结果证明谷物产量每在谷物收益变化为94.64%贡献的平方米与6.4t/hm2的一个平均数和谷物的数字从2.15～12.49t/hm2。为优秀特点，所有米饭异种有短尺寸(谷物长度≤5.5 ；公里)并且大胆形状(到宽度比率=1.10-2.00的谷物长度)。大多数米饭异种(87.5%)低于20%有PCG价值。有的MRY重视甚于50%的所有异种，低于20%的交流值，和低于10%的PC值。白垩的谷物的百分比否定地显著地与MRY被相关并且断然与DM相关。BRY和MRY否定地显著地与DM被相关。PC显著地并且断然与MRY被相关并且否定地与DM相关，当交流没这些优秀特点地有重要关联时。有25米饭异种，完成了象白垩的谷物的低百分比那样的江苏标准装饰用的梨树米饭的主要要求，这被结束，低直链淀粉满意的、最佳的蛋白质内容，并且它能被用作精英germplasms。因此，识别的异种可以在米饭质量的改进导致重要进步。

简介：为了推进，改进高地大米变化Huhan3和Huhan7，种子样品为约1和5d与二艘可重获的飞船被送到外层空间并且分别地为7和5代被宣传。Phenotypic分析表明词法特点和蛋白质和谷物的直链淀粉内容变化了。由联系基因的简单顺序重复(SSR)和插入删除(InDel)的genomic变化的描述标记显示变化模式是很复杂的。大多数变化在简单顺序重复碎片发生在碎片的3结束或5结束。反向的抄写聚合酶链反应(RT-PCR)试金证明在SSR的那些部分的变化影响了他们的基因表示，显示那基因联系了标记将有用孤立功能的基因。为繁殖的地调查也表明有高产量，高质量和干旱忍耐的更多的线能通过太空繁殖被选择。结果显示太空mutagenesis为米饭繁殖导致了分子的变化，以及生理、词法的变化。

简介：Riceblack-streakeddwarfvirus(RBSDV)isarecognizedmemberofthegenusFijivirus,familyReoviridae.Itsgenomehastendouble-strandedRNA(dsRNA)segments(S1-S10),inwhichthefifthgenomesegment(S5)containstwoopenreadingframes(ORFs)withapartiallyoverlappingregion.ThesecondORFofRBSDVS5encodesaviralnonstructuralproteinnamedp5bwithunknownfunction.Torevealthefunctionofp5b,itsgenewasligatedintothebaitplasmidpGBKT7andanexpressionlibrarycontainingricecDNAswasconstructedusingplasmidpGADT7foryeasttwo-hybridassay.Thebaitproteinp5bwasdetectedinyeastbywesternblot,andtheresultofanauto-activationtestshowedthatp5bcouldnotautonomouslyactivatetheexpressionofreportergenesinyeast.Thenthebaitproteinp5bwasusedforscreeningthecDNAexpressionlibrariesofrice.Genefragmentsofsomepivotalenzymesinvolvedinphotosynthesis,respirationandotherimportantmetabolicprocesses,wereidentifiedtointeractwithp5binyeast,suggestingthattheseinteractionsmayplayrolesinsymptomdevelopmentininfectedplants.

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简介：一张加倍的haploid(DH)人口由120根线组成了，源于在一个indica变化，TN1，和一个装饰用的梨树变化之间的一个十字，Chunjiang06，被用来识别由使用QTLNetwork软件在胚芽和3-leaf-seedling阶段控制米饭寒冷忍耐的QTL。在4以后的正常胚芽的百分比