学科分类
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42 个结果
  • 简介:Thepromoterregionofadroughtandabscisicacid(ABA)induciblegene,osr40c1,wasisolatedfromasalt-tolerantindicaricevarietyPokkali,whichis670bpupstreamoftheputativetranslationstartcodon.Insilicopromoteranalysisofresultedsequenceshowedthatatleast15typesofputativemotifsweredistributedwithinthesequence,includingtwotypesofcommonpromoterelements,TATAandCAATboxes.Additionally,severalputativecis-acingregulatoryelementswhichmaybeinvolvedinregulationofosr40c1expressionunderdifferentconditionswerefoundinthe5′-upstreamregionofosr40c1.TheseareABA-responsiveelement,light-responsiveelements(ATCT-motif,BoxI,G-box,GT1-motif,Gap-boxandSp1),myeloblastosisoncogeneresponseelement(CCAAT-box),auxinresponsiveelement(TGA-element),gibberellin-responsiveelement(GARE-motif)andfungal-elicitorresponsiveelements(BoxEandBox-W1).Aputativeregulatoryelement,requiredforendosperm-specificpatternofgeneexpressiondesignatedasSkn-1motif,wasalsodetectedinthePokkaliosr40c1promoterregion.Inconclusion,thebioinformaticanalysisofosr40c1promoterregionisolatedfromindicaricevarietyPokkaliledtotheidentificationofseveralimportantstress-responsivecisactingregulatoryelements,andtherefore,theisolatedpromotersequencecouldbeemployedinricegenetictransformationtomediateexpressionofabioticstressinducedgenes.

  • 标签: 启动子序列 计算机分析 籼稻品种 分离 顺式调控元件 CAAT盒
  • 简介:以便揭示在二CCDD染色体种,Oryzaalta和Oryzalatifolia之间起源和进化关系,在situ杂交(鱼)荧光被采用从O与C0t-1DNA分析二种染色体。alta作为根探针。Karyotype比较地也在O之间被分析。alta和O。latifolia基于他们杂交类似的乐队模式发信号。在O之间有高相同和靠近关系。alta和O。然而,在杂交之间区别表明latifolia也是清楚C0t-1DNA被证明是种类--并且染色体类型特定。C0t-1DNA鱼能是更有效分析在不同种类之间genomic关系,这被建议。根据在二allotetraploidy种之间高度并且中等重复DNA序列比较分析,O。alta和O。latifolia,可能起源和Oryzaallotetraploidy进化机制被讨论。

  • 标签: 重复DNA序列 D基因组 水稻 染色体核型分析 杂交信号 原位杂交
  • 简介:到在米饭germplasm91-1A2米饭胆量小蚊抵抗被识别并且遗传上分析了。米饭人口F1s从作为个男父母与米饭材料Jinggui,TN1,W1263(Gm1),IET2911(Gm2),BG404-1(gm3),OB677(Gm4),ARC5984(Gm5)和Duokang1(Gm6)交叉91-1A2被导出。到米饭胆量小蚊所有父母线和F1,BC1F1和F2人口抵抗被识别。结果证明91-1A2和所有F1s对中国米饭胆量小蚊遗传因子型IV抵抗。到在BC1F1和F2易受影响抵抗植物分离比率被X2测试与1:3和9:7规则给予,建议到中国米饭胆量小蚊遗传因子型IV91-1A2抵抗被是新抵抗基因二主导基因控制,对已知米饭胆量小蚊抵抗基因非突变而产生之遗传因子。

  • 标签: 水稻种质 遗传分析 稻瘿蚊 抗性鉴定 显性基因控制 F2群体
  • 简介:Smallubiquitin-likemodifier(SUMO)-conjugatingenzymesareinvolvedinpost-translationalregulatoryprocessesineukaryotes,includingtheconjugationofSUMOpeptidestoproteinsubstrate(SUMOylation).SUMOylationplaysanimportantroleinimprovingplanttolerancetoabioticstresssuchassalt,drought,heatandcold.Herein,wereportedtheisolationofOsSCE1(LOC_Os10g39120)geneencodingaSUMO-conjugatingenzymefromrice(Oryzasativacv.Nipponbare)anditsfunctionalvalidationinresponsetodroughtstress.TheE2enzyme,OsSCE1,isoneofthreekeyenzymesinvolvedintheconjugationofSUMOtoitstargetproteins.ActivatedSUMOistransferredtothecysteineofanE2enzymeandthentothetargetlysineresidueofthesubstrate,withorwithoutthehelpofanE3SUMOligase.ExpressionofOsSCE1wasstronglyinducedbypolyethyleneglycol6000(PEG6000)treatment,whichsuggestedOsSCE1maybeinvolvedinthedroughtstressresponse.OverexpressionofOsSCE1(OsSCE1-OX)inNipponbarereducedthetolerancetodroughtstress.Conversely,thedroughttolerancewasslightlyimprovedbytheknockdownofOsSCE1(OsSCE1-KD).TheseresultswerefurthersupportedbymeasurementofprolinecontentinOsSCE1-OXandOsSCE1-KDtransgeniclinesunderinduceddroughtstress,whichshowedOsSCE1-KDtransgeniclinesaccumulatedhigherprolinecontentthanthewildtype,whereasOsSCE1-OXlinehadlowerprolinecontentthanthewildtype.ThesefindingssuggestedOsSCE1mayplayaroleasanegativeregulatorinresponsetodroughtstressinrice.

  • 标签: Oryza SATIVA drought stress small ubiquitin-like
  • 简介:Cd忍耐和米饭幼苗translocation上H2O2预告处理效果用在Cd忍耐不同二米饭栽培变种(N07-6和N07-63)被学习。malondialdehyde(MDA)内容,减少谷胱甘肽(GSH),非蛋白质thiols(NPT),phytochelatins(PC)和谷胱甘肽S-transferase(GST)活动在暴露于各种各样处理二栽培变种之间被比较。结果证明50mol/LCd暴露显著地禁止了米饭生长,提高了GSH,NPT,PC和MDA生产,并且增加了GST活动,并且二栽培变种之间有重要差别。更多Cd被搬运进N07-6射击。H2O2预告处理由进步在根增加GSH,NPT和PC内容,以及GST活动减轻了Cd毒性。在N07-63这些参数增加度比在N07-6那些高,建议N07-63忍耐比N07-6更显著地被提高。氢过氧化物把Cdtranslocation归结为米饭射击,但是不同地在根影响了Cd内容。从上述结果,在到在二栽培变种之间H2O2预告处理Cddetoxification和反应有显著差别,这可以被推测。

  • 标签: 水稻品种 过氧化氢 预处理 CD 谷胱甘肽S 个人电脑
  • 简介:阳离子exchangers(CAX)属于广泛地在最后十在植物tonoplasts被调查了cation/Ca2+exchanger总科。最近,在植物涉及重金属累积和忍耐CAX角色植物救治和食物安全被学习了。在这微型评论,我们在Ca2+信号transduction总结Ca2+/H+antiporter角色,维持离子动态平衡并且扣押重金属进液泡。而且,我们在场在重金属detoxification血浆膜Ca2+/H+antiporter个可能角色。

  • 标签: 转运蛋白 逆向 质膜 耐受性 重金属积累 钙信号转导
  • 简介:有低glutelin内容瑞斯作为肾失败影响病人功能食物合适。在米饭低glutelin内容基因Lgc1有在二高度类似的glutelin基因GluB4和GluB5之间3.5-kb删除,它在染色体2短手臂上定位。在低glutelin内容米饭改进选择效率繁殖,指定为InDel-Lgc1-1和InDel-Lgc1-2二个分子标记被开发检测低glutelin内容基因Lgc1。双PCR察觉显示二个标记联合使用能容易把Lgc1遗传型与不同米饭变化区分开来。作为种简单、便宜技术,因此,分子标记能广泛地被用来与Lgc1基因识别不同变化并且在低glutelin内容米饭帮助标记选择适用。

  • 标签: 蛋白基因 水稻育种 分子标记 蛋白含量 ORYZA PCR检测
  • 简介:到怀有基因Pi-d2从pCB6.3kb,pCB5.3kb和pZH01-2.72kb三不同表示向量转变了米饭强风抵抗转基因米饭线米饭强风抵抗被分析。有Pi-d2基因九根先进产生转基因米饭线显示了各种各样抵抗到39米饭强风紧张,并且最高疾病抵抗频率到达了91.7%。有Pi-d2基因四根早产生同型结合转基因线展出了抵抗到58米饭强风紧张中超过81.5%个,显示出宽光谱抵抗特征。当在文化媒介粗略毒素集中增加了,米饭强风真菌粗略毒素选择转基因胚胎calli证明从转基因米饭植物不成熟胚胎胼胝正式就职率减少了。当粗略毒素集中到达了40%,从转基因线不成熟胚胎胼胝正式就职率是49.3%,并且受体控制是5%。在正式就职下面的地里转基因大米线颈强风疾病发生是0%~50%,显示转基因大米线大米强风抵抗比受体控制高得多。

  • 标签: 转基因水稻植株 水稻稻瘟病 抗性基因 Pi 幼胚愈伤组织 胚性愈伤组织
  • 简介:Tiller角度,个很必要农学特点,在米饭繁殖是重要,特别在植物类型繁殖。控制2个tiller角度(tac2)异种被乙醇甲烷磺酸盐mutagenesis从restorer线Jinhui10获得。tac2异种在幼苗阶段和显著地在tillering阶段增加tiller角度显示了正常显型。初步生理研究显示异种对GA敏感。因此,TAC2和TAC1可能以方法控制tiller角度,这被推测。基因分析证明变异特点被主要后退基因控制并且位于用SSR标记染色体9。在TAC2和它最近标记RM3320和RM201之间基因距离分别地是19.2厘米和16.7厘米。

  • 标签: 分蘖角度 遗传分析 水稻育种 隐性基因 突变体 SSR标记
  • 简介:在米饭restorer线C224条纹疾病抵抗继承特点加多基因用主要基因混合效果模型被分析。另外,抵抗与维护者线在C224七个十字被调查。结果证明C224条纹抵抗被二主要基因与添加剂优势效果(E-1模型)加多基因与additive-dominance-epistasis效果控制。这二基因有12.47%和24.75%分别地,出现否定优势效果添加剂效果。在二主要基因之间有重要epistasis和相互作用效果。当多基因是2.74%,二主要基因可遗传性是92.12%,显示条纹抵抗有主导主要基因效果。七个十字,五显示了高或中等抵抗到条纹疾病。

  • 标签: 主基因+多基因 混合效应模型 条纹叶枯病 水稻恢复系 遗传分析 上位性效应
  • 简介:Rice(Oryzasativa)issensitivetosalinity,butthesalttoleranceleveldiffersamongcultivars,whichmightresultfromnaturalvariationsinthegenesthatareresponsibleforsalttolerance.High-affinitypotassiumtransporter(HKTs)hasbeenproventobeinvolvedinsalttoleranceinplants.Therefore,wescreenedfornaturalnucleotidepolymorphisminthecodingsequenceofOsHKT1,whichencodestheHKTproteinineightVietnamesericecultivarsdifferinginsalttolerancelevel.Intotal,sevennucleotidesubstitutionsincodingsequenceofOsHKT1werefound,includingtwonon-synonymousandfivesynonymoussubstitutions.Furtheranalysisrevealedthatthesetwonon-synonymousnucleotidesubstitutions(G50TandT1209A)causedchangesinaminoacids(Gly17ValandAsp403Glu)atsignalpeptideandtheloopofthesixthtransmembranedomain,respectively.Toassessthepotentialeffectofthesesubstitutionsontheproteinfunction,the3DstructureofHKTproteinvariantswasmodelledbyusingPHYRE2webserver.Theresultsshowedthatnodifferencewasobservedwhencomparedthosepredicted3DstructureofHKTproteinvariantswitheachother.Inaddition,thecodonbiasofsynonymoussubstitutionscannotclearlyshowcorrelationwithsalttolerancelevel.Itmightbeinterestingtofurtherinvestigatethefunctionalrolesofdetectednon-synonymoussubstitutionsasitmightcorrelatetosalttoleranceinrice.

  • 标签: 水稻科学 农业
  • 简介:ThecompleteopenreadingframeofOsPIN1awasamplifiedthroughreversetranscriptase-polymerasechainreaction(RT-PCR)basedonthesequencedepositedinGenBanktoexploretherelationshipbetweentheauxineffluxproteinOsPIN1aandthenegativephototropismofriceroots.SequencingresultsshowedthattheGCcontentofOsPIN1awas65.49%.ThefusionexpressionvectorpCAMBIA-1301-OsPIN1a::GFPcontainingtheOsPIN1ageneandacodinggreenfluorescentprotein(gfp)genewasconstructed.ThefusionvectorwastransferredintoonionepidermalcellsbyAgrobacteriumtumefacienstransformation.ThetransientexpressionofOsPIN1a-GFPwasmainlylocatedinthenucleusandcellmembrane.Moreover,thetransgenicplantswereobtainedbyAgrobacterium-mediatedgenetictransformation.MoleculardetectionperformedbyusingPCRandβ-glucuronidasestainingshowedthatthetargetconstructwasintegratedintothegenomeofrice.Thenegativephototropiccurvaturesofthetransgenicricerootswerehigherthanthoseofthewildtype.Similarly,theexpressionlevelsofOsPIN1ainthetransgenicplantswereconsiderablyhigherthanthoseinthewild-typeplants.TheseresultssuggestthatOsPIN1aiscrucialinthenegativephototropiccurvatureofriceroots.

  • 标签: RICE OsPIN1a green FLUORESCENT protein TRANSIENT
  • 简介:Byusing304recombinantinbredlinesderivedfromindicaricecrossZhong156/Gumei2,alinkagemapconsistingof177markerlociandcovering12ricechromosomeswasconstructedandemployedformappinggenesconferringblastresistanceinrice.GenomiclocationofgenePi25(t)conferringneckblastresistancetotheChineseisolate92-183(raceZC15)wasverifiedtobelocatedbetweenmarkersA7andRG456onchromosome6,withgeneticdistancesof1.7cMand1.5cMtoA7andRG456,respectively.LeafblastresistanceofGumei2tothePhilippineisolateCa89(lineage4)wasfoundtobecontrolledbyasinglegene.ThegenetentativelydesignatedasPi26(t)waslocatedbetweenmakersB10andR674onchromosome6,withgeneticdistancesof5.7cMand25.8cMtoB10andR674respectively.ResistantallelesatbothgenelociwerederivedfromGumei2,indicatinganexistenceofresistancegeneclusterinGumei2.

  • 标签: 水稻 稻瘟病 抗病性 分子标记 基因簇 Gumei
  • 简介:Membersoftheactivityofbc1complex(ABC1)familyareproteinkinasesthatarewidelyfoundinprokaryotesandeukaryotes.PreviousstudiesshowedthatseveralplantABC1genesparticipatedintheabioticstressresponse.Here,wepresentthesystematicidentificationofriceandArabidopsisABC1genesandtheexpressionanalysisofriceABC1genes.Atotalof15and17ABC1genesfromthericeandArabidopsisgenomes,respectively,wereidentifiedusingabioinformaticsapproach.Phylogeneticanalyseso...

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  • 简介:米饭镉(Cd)敏感变异cadB-1用Agrobacteriumtumefaciens被获得调停系统。在cadB-1和野类型(WT)暴露以后米饭幼苗到有增加外部Cd集中cadB-1和WT10d,在根积累到高水平Cd,茎和叶子Cd集中个范围,并且在cadB-1幼苗生长抑制比在WT更严肃。氢过氧化物累积在cadB-1叶子和根是更高。减少谷胱甘肽(GSH)比率/oxidized谷胱甘肽(GSSG),ascorbate(ASC)/dehydroascorbate(DHA)和减少菸碱腺嘌dinucleotide磷酸盐(NADPH)/oxidized菸碱腺嘌dinucleotide磷酸盐(NADP+)在高Cd层次下面在叶子和根两个都比在WT在cadB-1是更低。ascorbateperoxidase(APX)活动,谷胱甘肽peroxidase(GR),dehydroascorbatereductase(DHAR)和monodehydroascorbatereductase(MDHAR)在Cd高水平处理下面在叶子和根两个都比在WT在cadB-1是也更低。我们结果建议在Cd应力下面,ASC-GSH周期更严重比在WT在cadB-1被禁止,显示变异cadB-1不太能清除反应氧种类并且对Cd敏感。

  • 标签: 脱氢抗坏血酸还原酶 还原型谷胱甘肽 镉(CD) 水稻幼苗 突变体 还原型烟酰胺腺嘌呤二核苷酸
  • 简介:个实验被进行用荧光灯微分显示器(软式磁碟机)方法在干旱应激和正常条件下面在米饭叶子和根比较信使rna表达式差别。积极碎片被H.A联合孤立黄页(contained0.1%H.A。黄)分离和宏数组屏蔽方法。比作ArabidopsisthalianaNADPH氧化还原酶基因,它有96%身份。cDNA是1423bp,并且包含了与345氨基酸残余编码蛋白质1048bp个完全读物框架。而且,基因表示水平在正常条件下面比那在干旱应激下面是更高。在干旱反应下面的NADPH氧化还原酶基因可能角色也被讨论。

  • 标签: 水稻 干旱压力 还原型辅酶II类氧化还原酶基因 克隆化 基因表达
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