简介：MicroRNAs(miRNAs)是非编码的小RNA，它响应关於生命、不能生活的压力起重要规章的作用。Dongxiang野米饭(Oryzarufipogon，DXWR)能在极端干旱环境幸存，但是它干旱抵抗的分子的机制大部分仍然是未知的。为了进一步探索miRNA，规章的机制在干旱抵抗包含了，我们用小RNA定序和生物信息学途径在DXWR识别了138新奇miRNAs，并且发现67新奇miRNAs的表示层次被干旱应力signicantly影响。总共，200候选人目标基因为干旱被预言并且注解压力应答的新奇miRNAs。基因本体论(去)小径建议了的基因和染色体(KEGG)的分析和京都百科全书大多数目标基因与新陈代谢有关。高展出的茎环量的即时PCR(qRT-PCR)结果与定序数据索引，哪个miRNA表示模式基于在现在的学习定序的小RNA是可靠的conrmed。同时，qRT-PCR验证了在几miRNAs和他们的目标基因之间的反的表示模式。这些结果将提高我们在DXWR响应干旱应力理解规章的机制miRNA，并且能为这个珍贵基因资源的保护和利用用作一本重要参考书。

简介：Outof1989wildaccessionssowninseedboxesforscreening,only1003wildaccessionswithgoodgerminationwerescreenedagainstbrownplanthopper(BPH),Nilaparvatalugens(St?l)undergreenhouseconditions.Thecollectioncomprisedofaccessionsfrom11wildspeciesandAfricancultivatedrice.ThegermplasmwasscreenedforBPHfollowingstandardseedboxscreeningtechniqueinthegreenhouse.Asmanyas159accessionswereidentifiedasresistantduringtheyear2012basedononeyearscreening.AselectedsetofBPHresistantaccessionswerescreenedagainduring2013.Basedonthetwoyearsscreening,sevenaccessionsofO.nivara(AA),oneaccessionofO.officinalis(CC),sevenaccessionsofO.australiensis(EE),fiveaccessionsofO.punctata(BBandBBCC)andnineaccessionsofO.latifolia(CCDD)wereconfirmedtoberesistanttoBPH.SofarnoBPHresistancegeneshavebeenidentifiedanddesignatedfromO.nivaraandO.punctata,hencethesemayactasnewsourcesofresistance.

简介：Thisstudywasconductedtodevelopandcharacterizeanovelcytoplasmicmalesterile(CMS)sourcewhichwasidentifiedfromDongxiangwildrice(Oryzarufipogon)bycrossingDongxiangwildriceasfemalewithZhongzao35,anindicainbredvariety,asmaleandcontinuousbackcrossingwithZhongzao35.ObservationunderopticalmicroscopemanifestedthatthisnovelCMSbelongedtotypicalabortiontypewithlesspollencomparedwithwildabortivetypecytoplasm(CMS-WA).SequentialplantingshowedthatthisnovelCMShascompleteandstablemalesterility.Testcrossexperimentshowedthatallthe24testedmaterialsincludingmaintainerandrestorerlinesofCMS-WAandHongliantypecytoplasm(CMS-HL)andotherindicainbredvarietiesarethemaintainerswithcompletemaintainingability,suggestingthatthisnovelCMShasfertilityrestorationtotallydifferentfromCMS-WAandCMS-HLandbelongstoanoveltypeofCMS.Sofar,weonlydiscoveredauniquefertilityrestorationsourceforthisnovelCMS.InheritanceanalysisshowedthatthefertilityrestorationofthisCMSwasgovernedbythreepairsofindependentdominantgenes.ProspectforapplicationofthisnovelCMSsysteminhybridricebreedingwasalsodiscussed.

简介：Alargenumberofplantremainswerediscoveredintheancient-woodslayerofZhujiajianIsland,ZhejiangProvince.Thereweresomethicktrunks,completelaminas,fruit,seedsandsoon.Accordingtoradiocarbontestsconductedforplantremains,theancient-woodslayerhasbeendatedbacktoabout8750-6200years,andthevegetationwasasubtropicalevergreenanddeciduousbroad-leavedmixedforestsontheislandinthepast.Inthemiddleoftheancient-woodslayer,twograinsofwildricewereexploredaccidentally,whichareOryzarufipogon,alongwiththefruitandseedsofsomewaterplants,suchasCeratophyllumdcmcrsum,C.oryzetoum,Euryalefetox,Trapaincisavar.quadricaudata,Scirpusyagaraandsoon.Theremightbemarshysoilandapondinancientforestvegetationfromwherethegrainsofwildriceandhydrophyticfruitwerefound.ItisoftremendousimportancetostudytheoriginofwildriceinChina.