简介：BACKGROUND:Previousresearchhasrevealedthatsomatostatincaninduceepilepsy,andthatthelevelsofneuropeptideYmayincreaseandbecomemoreactiveinbrainareaswithepilepticseizures.OBJECTIVE:ToobservetheeffectofGanodermalucidumsporepowderontheneuropeptideYandsomatostatincontentinthecerebralcortexandhippocampalregionsofseizureratsinducedbypentylenetetrazol(PTZ).Furthermore,toverifyanyeffectofGanodermalucidumsporepowderoninhibitionofepilepticseizures.DESIGN,TIMEANDSETTING:ArandomizedgroupanimalstudywasperformedinAugust2007intheSchoolofBasicMedicalSciences,JiamusiUniversity(Jiamusi,Heilongjiang,China).MATERIALS:Thirtyhealthy,male,Wistarrats,aged12weeksandweighing180–220g,weretakenastheexperimentalanimals.PTZ(SigmaCompany,UnitedStates)wasusedtoinduceepilepsy.Ganodermalucidumspores(Leyss,exFrvariety)werepurchasedfromJiamusiCityWildGrowingCaseoftheGanodermaLucidum(China).Rabbitanti-somatostatinantibodiesandsecondaryantibodieswerepurchasedfromWuhanBosterCompany(China).NeuropeptideYradioimmunoassaykitwaspurchasedfromBeijingFuruiBiotechnologyCompany(China).METHODS:Thirtyratswererandomlydividedintothreegroups:acontrolgroup,anepilepsymodelgroupandaGanodermalucidumspore-treatedgroup.Eachgroupcontained10animals.RatsintheepilepsymodelgroupweretreatedwithintraperitonealinjectionsofPTZandgastricperfusionofphysiologicsaline.IntheGanodermalucidumspore-treatedgroup,intraperitonealinjectionofPTZandgastricperfusionofGanodermalucidumsporepowderwereadministered.Theblankcontrolgroupwasonlyadministeredwiththephysiologicalsalinebyintraperitonealinjectionandgastricperfusion.MAINOUTCOMEMEASURES:ImmunohistochemicalstainingandradioimmunoassaymethodswereusedtoobservethechangesofsomatostatinandneuropeptideYcontentinbraintissueofepilepticrats,aswellasthemorphologyofneurons