简介:AbstractWith the deepening of research, proteomics has developed into a science covering the study of all the structural and functional characteristics of proteins and the dynamic change rules. The essence of various biological activities is revealed from the perspectives of the biological structure, functional activity and corresponding regulatory mechanism of proteins by proteomics. Among them, phospholipid-binding protein is one of the hotspots of proteomics, especially annexin A1, which is widely present in various tissues and cells of the body. It has the capability of binding to phospholipid membranes reversibly in a calcium ion dependent manner. In order to provide possible research ideas for researchers, who are interested in this protein, the biological effects of annexin A1, such as inflammatory regulation, cell signal transduction, cell proliferation, differentiation and apoptosis are described in this paper.
简介:ObjectivesToexploretheprotectiveeffectofirbesartan(Irb)undertheinterferencewithangiotensinⅡ(AngⅡ)onABCA1.MethodsElectronmicroscopywasusedtodetectthemorphousoffoamcells.TheexpressionofABCA1mRNAanditsproteinweredeterminedbyRT-PCRandWesternblotting,respectively.Thevarianceofcellularcholesterolcontentwasmeasuredbyzymochemistryvia-fluorospectrophotometer.ResultsApositivefacilitativeeffectofAngⅡontheformationoffoamcellswasobserved.TotalcholesterolwasincreasedsignificantlybyAngⅡ,theexpressionofABCA1wasdown-regulatedobviouslybyAngⅡ;IrbcouldprotectABCA1againstthelesionofAngⅡ;TotalcellularcholesterolcontentwasreducedsignificantlyinIrb+AngⅡgroup;However,considerablealterationaboutthecholesterolcontentandtheexpressionofABCA1werenotobservedinIrbgroupwithoutincubationwithAngⅡ.ConclusionsIrbcouldprotectABCA1againstthelesionofAngⅡ,whichmaycontributetoitsanti-atheroscleroticproperties.
简介:摘要:针对重庆仙桃数据谷A1塔楼应用MIDAS Building 对其进行6度0.05g地震作用下的非线性动力反应分析,依据计算结果从结构的基底剪力、层间位移角以及结构构件的塑性发展情况分析结构的整体作用响应及存在的薄弱部位;从构件层面验算关键构件的设计承载能力是否满足地震作用力作用所产生的内力需求,并依据分析结果对其进行改进。计算结果表明:在大震作用下,层间位移角满足规范要求;构件的塑性铰分布合理,有利于结构抗震;结构构件的承载能力均大于作用内力,确保结构实现预期的抗震性能目标。
简介:珍梅的阿爹和玛梅的阿妈,自就更不会去注意这件事了。生活的重压,使他们背朝天公面朝水已成为常态。必须多捕鱼多运货才能生存,才能让两个女儿读书,有起码的生活尊严。