简介：Anewopticalfiberfluorometricthermometerbasedonthetemperaturedependenceofthefluorescencelifetimeofphosphorisdescribed.Thephase-lockeddetection(PLD)systemisusedtomeasurefluorescencelifetime.Thecharacteristicsofthermmeterarediscussedandtheexperimentresultsaregiven.

简介：Vesiclecanbepreparedfromaqueousmixturesofsimplecommerciallyavailable,single-tailedcanonicandanionicsurfactants.Inthiswork,theI3/I1value,Ie/Imvalue,andfluorescencelifetimeofpyreneindifferentsystems(seethepreparationofsamples)weredetermined.Hieessentialaffectingfactorsintheformationofvesiclecanbededucedfromtheobtainedresults.Itshowedthatlargevesiclemustformnaturallybeforesonicationin0.082MoctyltrimethylammoniumbromideandsodiumlauratepH=9.2aqueoussolution.Whileaftersonication,onlysmallvesicleexists,whichcanbeprovedfurtherthroughelectronmicroscope.

简介：Objective:Infectionofhumanpapillomavirusincondylomaacuminatum(CA)wasdetectedbyrealtimefluorescencequantitativePCR(FQ-PCR)technique.Methods:SpecimensofCA-DNAquantificationfrom94caseswereexaminedbyrealtimeFQ-PCRtechniqueand32caseswerecomparedwiththesamemethodafter10-daystreatment.Results:CA-DNAwasfoundinallpatients,withanaverageof4.0×10^6copies/ul.After10daysoftreatment,theaveragewas2.1×10^5copies/ul.TherewasasignificantdifferenceintheaverageamountofCA-DNAbeforeandafterthetreatment.Conclusion:RealtimeFQ-PCRisagoodmethodforexaminingCA-DNAamountanditcandirectthetreatmentofCA.

简介：α-andβ-cyclodextrinsconsistingofsixandsevenglucoseresiduesrespectively,havelipophiliccavitieswithdifferentinnerdiameters.Theyformhost-guestinclusioncomplexeswithhydrophobicorganicandorganometallicguestmoleculesinaqueoussolution.Thesehost-guestcomplexeshaveprovedtobeexcellentmodelsystemsforstudyingthenatureofnoncovalentbondingforcesinaqueousmedia.TheyhaveprovidedvaluableinsightsintothehydrophobiceffectandLondondispersionforcesandaregoodmodelforunderstandingthespecificityofenzymesubstrateinteractions[1]Evidencefortheformationofinclusioncomplexeshavebeenprovidedfromcalovimetrictitration[2]NMR[33],circulardichroism[4],UV[1]andfluorescencespectra[5]andconductometricmethod[6]etc.Herewereportanewfluorimetricmethodforastudyonthereactionofthehost-guestinclusioncomplexesofcyclodextrinwithphenols.Dissociationconstants(Kd)oftheinclusioncomplexesofsomephenolswithα-β-cyclodextrinareestimatedbasedonthevariationofthefluorescentintensityandmodifiedHarad'equations.

简介：在氧气体的处理以后，包含胺组的小分子能射出荧光。氧化被相信在荧光中心的形成起一个重要作用。比作以前的结果，小分子和大分子可能有一样的荧光中心。

简介：ＩｎｔｒｏｄｕｃｔｉｏｎＡｖａｒｉｅｔｙｏｆｎｅｗｐｏｌｙａｍｉｎｏｐｏｌｙｃａｒｂｏｘｙｌａｔｅｌｉｇａｎｄｓａｎｄｔｈｅｉｒｌａｎｔｈａｎｉｄｅｃｏｍｐｌｅｘｅｓｈａｖｅｂｅｅｎｓｙｎｔｈｅｓｉｚｅｄｉｎｒｅｃｅｎｔｙｅａｒｓｂｅｃａｕｓｅｏｆｔ...

简介：荧光刺激和可溶的有机物(WSOC)样品从20提取了的46水的平均分子的重量在东方中国玷污类型被决定。它被发现所有样品分享类似的光谱学。一种好线性关系不过在350～450nm的范围在全部的器官的碳和刺激之间存在器官的碳的内容和样品的pH在一个宽范围变化。在相对刺激紧张和WSOC和FA的平均分子的重量之间的重要关联都没被发现，但是部分关联为WSOC样品作为控制因素与pH变得重要。相对刺激紧张显示出在学习区域从南方增加到北方的一个一般趋势。pH价值可能在调整起一个重要作用荧光灯WSOC的空间变化。

简介：Ourrecentstudiesconcerningthebindingofionicsurfactantsonoppositelychargedpolyelectrolytesobservedwithfluorescencetechniquesarereviewed.Thecationicsurfactantscetyltrimethylammoniumbromide(CTAB),dodecyltrimethylammoniumchloride(DTAC),andnonionicsurfactantoctaethyleneglycolmonododecylether(C12E8)wereallowedtobindonanionicpoly(2-acrylamido-2-methylpropanesulfonicacid)(PAMPS)anditspyreneand/ornaphthalenelabeledcopolymers.TherelativeexcimeremissionintensityIE/IMofacationicprobe1-pyrenemethylaminehydrochloridewerechosentomonitorthebindingprocessandtheconformationchangeofsurfactant-boundpolyelectrolytes.The1:1aggregationofpolyelectrolyte-CTABwithrespecttothechargewasfoundaslongastheCTABconcentrationwasslightlyhigherthanitscriticalaggregationconcentration(CAC).TheintermolecularNRETindicatedthattheCTAB-boundpolyelectrolytesaggregatedtogetherthroughthehydrophobicinteractionbetweentheCTABtails.However,neither1:1polyelectrolyte-DTACaggregationnorintermolecularaggregationofDTAC-boundpolyelectrolytewasobservedowingtoitsweakerhydrophobicityof12carbonatomsinthetail,whichisshorterthanthatofCTAB.Asknownfromthefluorescenceresults,nonionicsurfactantC12E8didnotbindontheanionicpolyelectrolytes,butthepresenceofPAMPSpromotedthemicelleformationforC12E8attheCACslightlybelowitscriticalmicelleconcentration(CMC).ThesolidcomplexofdansyllabeledAMPScopolymer-surfactantexhibitedadecreaseinlocalpolaritywithincreasingchargedensityofthepolyelectrolyteorwithalkanetaillengthofthesurfactant.SAXSsuggestedalamellastructurefortheAMPScopolymersurfactantsolidcomplexeswithalongperiodof3.87nmforCTABand3.04nmforDTAC,respectively.

简介：一种荧光眼纤维的温度计基于红宝石和吸收玻璃样品被设计。眼--纤维温度测量探查基于红宝石被开发。这个系统对在0℃to的范围的温度测量特别地能适应130℃。更加改进的表演在这台新设备被看见。到带的罐头的驱动电流容易通过控制电路在要求的定义界限以内被保留。

简介：Introduction.Poly(N-vinylcarbazole)(PVCZ)hasreceivedmuchattentionasarepresentativephotoconductivepolymerandextensivestudiesonmechanical,physical,chemicalandelectricalpropertieshavebeenreported'’.Ingeneral,thePVCZobtainedbyradicalandordinarycanonpolym...

简介：Mitoxantrone,aclinicallyusefulantitumourantibioticforleukaemiaandbreastcancer,hasreceivedmoreattentions.Inthispaper,theinteractionbetweenmitoxantroneandcalfthymusDNAisinvestigatedbyRamanandfluorescencespectroscopies,andthebindingsiteofmitoxantronetocalfthymusDNAisexplored.TheresultsshowedthatmitoxantroneinteractswithcalfthymusDNAbasesbytheintercalationofanthracyclineintothebasepairplaneofadenine(A)andthymine(T),anditresultsinthedisruptionofthehydrogenbondsbetweencalfthymusDNAbases,andthusthecalfthymusDNAdouble-strandcanbedisruptedintotheB-formDNAdouble-strandsegments.

简介：ThetitlecomplexEu(Ⅲ)(TTA)3(phen)(whereTTA=thenoyltrifluoroacetonemonoanion;phen=1,10-phenanthroline)hasbeensynthesizedinmixedsolventsofacetoneandethanol(1:1volumeratio)anditscrystalstructurehasbeendeterminedbyX-raydiffraction.Thecomplexcrystalsaretriclinic,spacegroupP1(#2)withcelldimensionsofa=1.3241(2)nm,6=1.5278(4)nm,c=0.9755(3)nm,α=92.49(2)°,β=102.57(2)°,γ=91.62(2)°,V=1.9268(8)nm3,Z=2,μ(MoKα)=18.77cm-1,Dx=1.720g/cm3.ThecoordinationgeometryofEuatomisadistortedsquareantiprism,andtheencapsulatedstructurethatcanmeetthestructuralrequirementofthetypicaleuropiumluminescentsensor.Thefluorescencespectrumsuggeststhatthecomplexisastrongphotoluminescentmaterial.

简介：TwokindsofSt/DVBcopolymerbeedscontaining(1-pyrenyl)formyland(1-pyrenyl)methylgroupsrespectivelyhavebeensynthesizedbybothfunctionalizationandcopolymerization.Thefluorescencespectraoftheswollenpolymerssynthesizedaresimilarinshapetothoseofthecorrespondingmodelcompounds,whiletheintensityratioofexcimertomonomershowscleardependenceonthecontentsofthepyrenegroups.

简介：Cholesterolmodifieddextran(CHD)havingself-aggrgationorself-assemblypropertywassynthesizedfromcholesteroland1,6-hexyldiisocyanate.Thedegreeofsubstitutionofcholesterylmoietyindextranmainlineis3-5cholesterolsthe100glucoseunits.WehavepreparedwatersolutionofCHDusingprobetypesonifierandN-Phenyl-a-naphthylamine(PNA)asafluorescentprobetostudyCHDself-aggregateprocess.Foreachsolutionoftwosamples,wefoundthatthemaximumemissionofPNAinCHDconcentration.Thischangecorrespondstotheformationofmicelle-likeclustersself-aggregatedbythecholesterolmoietyoncetheCHDconcentration.Thischangecorrespondstotheformationofmicelle-likeclustersself-aggregatedbythecholesterolmoietyoncetheCHDconcentrationexceeds0.01mg/ml.

简介：ＩｎｔｒｏｄｕｃｔｉｏｎＴｈｅｄｅｔｅｒｍｉｎａｔｉｏｎｏｆｃｈｌｏｒｏｔｅｔｒａｃｙｃｌｉｎｅ（ＣＴＣ）ｈａｖｅｍａｉｎｌｙｂａｓｅｄｕｐｏｎｃｈｒｏ－ｍａｔｏｇｒａｐｈｉｃ［１］，ｓｐｅｃｔｒｏｐｈｏｔｏｍｅｔｒｙ［２］，ｖｏｌｔａｍｍｅｔｒｙ［...

简介：Heptacyclo[6.6.0.02,6.03,13.04,11.05,9,010,14]tetradecaneisacage-shapedmoleculeofbothhighsymmetry(D2d)andhighrigidity.Inourpreviousreports,ithasbeenusedasanuniquespacergroupforelectrontransferprocessesbetweenadonor(D)andanacceptor(A)substituent.TheDandAchromophorescanbealignedalongastraightline,eithercoplanar(0°dihedralangle)orperpendicular(90°dihedralangle)withrespecttoeachother.Knowledgecollectedinthisworkmaybeusefulforthedesignofphoto-electronicdevices,suchaselectrochemicalsensors,photovoltaiccells,molecularrectifiersandswitches,etc.

简介：Thebinarycomplexesofeuropiumwithbenzoicacidanditsderivatives(phthalicacid,m-phthalicacid,o-aminobenzoicacid,salicylicacid,andsulfosalicylicacid)weresynthesizedandtheircompositionswereidentifiedbyelementalanalyses.UVandIRofthecomplexeshavebeeninvestigated.TheUVspectraindicatedthatthecomplexes'ultravioletabsorptionweremainlytheligands'absorption.TheIRspectrashowedthattheIRspectraofcomplexesaredifferentfromthoseoffreeligands.Thefluorescencepropertiesofthemwereinvestigatedbyusingluminescencespectroscopy,theresultsshowedthatonlythreecomplexesappearasbetterluminescence,theywereEu-benzoicacid,Eu-m-phthalicacidandEu-phthalicacid,whiletheothersexhibitedtheligands'widebandemission.

简介：ThemorphologicalchangeofSt/DVBcrosslinkedgelsduringchloromethylationwasstudiedbyfluorescencespectroscopyusingSt/DVBcrosslinkedandhypercrosslinkedgelsascontrolsamples.Ithasbeenfoundthatwithincreaseofchlorinecontent,theexcimeremissionband(～325nm)approachestovanish,whiletheintensityofmulti-ringaggregateemissionband(～420nm)quicklyreachesamaximum,andthendecreasessharplyaccompaniedbyappearanceofanewbroadbandcentredatca488nmwhichroughlycoincideswiththetypicalemissionbandofhypercrossllinkedSt/DVBgels.Mearwhile,theresultofIRmeasurementsuggeststhatmethylenebridgebetweenphenylringsformsandincreaseswithchloromethylationprocess.Theseresultsareexplainedintermsofasidereactionofpost-crosslinking,whichdensifiesthelooselycrosslinkednetworksandundoesthedenselyentangledmicrogelnuclei.Asaresult,themorphologyofthecrosslinkedgelsbecomesmorehomogeneouswithchloromethylation.

简介：FluorescencequenchingofjanusgreenB(JGB)insodiumdodecylsulfate(SDS)micellebynucleicacids(DNA)wasstudiedusingUV-visabsorption,steadystatefluorescenceemissionmethodsandlifetimemeasurements.IntheSDSmicelle,weakfluorescenceofJGBwasenhanced,andthemaximumemissionshiftedfrom425to410nm.InthepresenceofDNA,thefluorescenceofJGBwasquenched.Linearrelationshipsbetweenthefluorescencequenching(F0/F)andconcentrationsofDNAwereobservedintherangeof2.4×10^-8to1.08×10^-7mol·L^-1forcalfthymusnucleicacids(ctDNA)and1.9×10^-8to3.8×10^-8mol·L-1forfishspermnucleicacids(fsDNA)when2.5×10^-5mol·L^-1JGBwasemployed.Thelimitdetectionwere1.3×10^-8mol·L^-1forctDNAand6.4×10^-9mol·L^-1forfsDNA.AthighDNAconcentration,therewasasystematicdeviationfromtheStem-Volmerequationduetothestaticanddynamicquenchingoccurringsimultaneously.TheproposedmethodwasappliedtothedeterminationofthenucleicacidsinchickenbloodextractionandtheanalyticalresultswereingoodagreementwiththeUV-method.

简介：Inthispaper,itwasdiscoveredthatanovelpH-sensitivecopolymerofN-isopropylacrylamide(NIP)andN-(3-dimethylaminopropyl)methacrylamide(DMAPM)couldbegottenbypolymerization.ThephasetransitionpH(pHtr)ofP(NIP-DMAPM)polymerwasfoundtobe7.4at37℃.ThepolymerwasprecipitatedoutofwateraboveacriticalpH=7.4andre-dissolvedbelowpH----7.4.Thecharacteristicofthispolymermadeitpossibletocarryouttheimmunochemicalstepsofanimmunoassayinatruesolutionandthentoquicklyseparatetheresultingproductfromthereactionmixture.Inacompetitivefluorescenceimmunoassay,thestandardrabbitIgGandrabbitIgGimmobilizedonP(NIP-DMAPM)firstcompetitivelyreactedwiththefluoresceinisothiocyanate(FITC)labeledantibody,thenthepHofsolutionwasadjustedabovethepHtrofpolymertoprecipitatethepolymer-immunecomplex,andthepolymer-immunecomplexprecipitatewasseparatedandre-dissolvedbytheadjustmentofpH,finallytheFITC-labeledantibodyintheimmunecomplexwasquantifiedbyfluorescencemeasurement.ThecalibrationgraphforrabbitIgGwaslinearovertherangeof100-1000ng/mLwithadetectionlimitof11ng/mL.Themethodisrapid,sensitiveandsimple.OwingtoneutralpHtrofP(NIP-DMAPM),thedamagetoantigen-antibodyimmunecomplexwasgreatlydecreasedinthecourseofseparation.Inaddition,asandwichenzyme-linkedfluorescenceimmunoassaymethodforthedeterminationofhumanIgGwasalsodeveloped,showingthatthepH-sensitivephaseseparatingimmunoassaycouldbeperformedinthecompetitivemethodaswellasthesandwichmethod.