简介:Epicuticularwaxformstheoutermostprotectivebarrieroftheaerialsurfacesoflandplants,workinginconcertwithothercomponentsoftheplantcuticletopreventuncontrolledlossofwaterandtoprovideprotectionagainstanarrayofexternalenvironmentalstress.Inthisstudy,chemicallymutagenizedpopulationsofrice(OryzasativaL.)derivedfromapproximately4750M2familieswerescreenedforadhesionofwaterdropletsresultinginawetleaf/glossy(wlg)phenotype.Mutantswereidentifiedin11independently-derivedM2families.Scanningelectronmicroscopyanalysisconfirmedtheassociationofthewlgphenotypewithchangesintheepicuticularwaxcrystalsoftheseplants.Thephenotypesoffivemutants(7-17A,26.1,524.2,680.2,and843.1)wereconfirmedtobetheresultofsinglerecessivegenemutation.Evaluationofmutantsfrom3(6-1A,7-17A,and11-39A)of11M2familiesrevealedsignificantreductions(>50%)insurfacewaxcontentandincreasesincuticlemembranepermeability.
简介:到米饭异种的分子的水平上的变化的特征在空间环境导致了的进一步的学习,我们在四米饭异种(二high-tillering和二low-tillering)的叶子和种子分析了蛋白质在8(th)并且9(th)在一架15天的空间班机以后的代,并且与他们由two-dimentionalpolyacrylamide的扎根的控制相比,凝胶电泳和颠倒分阶段执行液体层析(RPLC)。另外,变异的种子的白朊,血球素,谷醇溶蛋白,谷蛋白,和直链淀粉被RPLC和紫外线spectrometry分析。结果证明在山峰的叶子的低丰富的蛋白质是到ering舞台为止更可能的与他们的相应控制相比被导致。当时,揭示的变异的种子的白朊,血球素,和谷醇溶蛋白变化稳定地与他们在不同异种的变化的控制,和特征相比被继承在8(th)并且9(th)代,建议他们能被用作简历标记到异种由空间飞行导致了的身份。而且,二蛋白质(SSP9111和SSP6302)被发现在不同异种与高紧张(双重的变化)被表示,它两个都根据集体spectrometry并且数据库寻找与相片系统被相关。
简介:Elevennitratenon-utilizing(nit)mutantswererecoveredfromsixisolatesofMagnaporthegriseaculturedonMMmedia,amendedwith60g/Lpotassiumchlorate,withafrequencyof1.42°.Somebiologicalproperties,suchasgrowthrate,growthbiomass,culturalcharacters,conidialproduction,sexualreproductionability,andpathogenicitywerecomparedbetweennitmutantsandtheirparentisolates.Resultsshowedthatallthenitmutantswereresistanttochlorate.SomeimportantbiologicalpropertiessuchasthegrowthrateonYPSA,conidialproductionabilityonTPSA,pathogenicity,hadnosignificantdifferencesbetweennitmutantsandtheirparentisolates.Matingtypedidn'tchange,butperitheciaproductionabilityoffertileisolateschangedsignificantlyascomparedwiththatoftheirparentisolates.Therefore.thenitcanbeusedasageneticmarkertostudythegeneticssuchaspathogenicity,fungicideresistanceinMagnaporthegrisea.
简介:到精选精英germplasms,从装饰用的梨树米饭栽培变种Wuyujing3导出的112异种被评估。象圆锥花序那样的收益部件每平方米数,谷物数字每圆锥花序,和谷物,重量被测量。象白垩的谷物(PCG)的百分比那样的优秀特点,稻谷收益(BRY),milled米饭收益(MRY),milling(DM)的度,直链淀粉内容(交流),蛋白质内容(PC),和在特点之中的关系是inverstigated。结果证明谷物产量每在谷物收益变化为94.64%贡献的平方米与6.4t/hm2的一个平均数和谷物的数字从2.15~12.49t/hm2。为优秀特点,所有米饭异种有短尺寸(谷物长度≤5.5 ;公里)并且大胆形状(到宽度比率=1.10-2.00的谷物长度)。大多数米饭异种(87.5%)低于20%有PCG价值。有的MRY重视甚于50%的所有异种,低于20%的交流值,和低于10%的PC值。白垩的谷物的百分比否定地显著地与MRY被相关并且断然与DM相关。BRY和MRY否定地显著地与DM被相关。PC显著地并且断然与MRY被相关并且否定地与DM相关,当交流没这些优秀特点地有重要关联时。有25米饭异种,完成了象白垩的谷物的低百分比那样的江苏标准装饰用的梨树米饭的主要要求,这被结束,低直链淀粉满意的、最佳的蛋白质内容,并且它能被用作精英germplasms。因此,识别的异种可以在米饭质量的改进导致重要进步。
简介:客观:击倒链球菌mutans(S.mutans)的全部勒克司基因经由相应再结合和构造的UA159紧张S的删除勒克司的变异的紧张。Mutans。学习S的酸抵抗之间的差别。MutansIngbrittC国际标准紧张和勒克司变异的紧张的酸抵抗。方法:二DNA碎裂定位在勒克司上面、下游基因被放大,在他们之间的PJT10的抗生素的一种抵抗基因被设计进PUC19为构造再结合的plasmidplasmidpUCluxKO。S.mutans房间与的ElectrotransformationpUCluxKO变异导致了抗生素的一种的隔离抵抗S。Mutanstransformants,它被聚合酶链反应,V.harveyiBB170光生物鉴定和定序的分析识别。S的答案。有一样的密度的Mutans标准紧张和勒克司变异的紧张被做并且在pH有教养为一样的period.Terminal生长状况的3.5~7.0BHI液体compared.Firstly在pH被酸化5.5BHI液体,二紧张在pH是有教养的3.0BHI液体。二紧张的酸忍耐回答是compared.Results:限制endonuclease分析证明那pUCluxKO变异的向量成功地被重新结合。S.mutans异种的删除勒克司的地位被PCR与为勒克司和抗生素的一种抵抗的基因特定的教材证实。S.mutans异种不能导致生物体之发光,indiating异种成功地被重新结合。在文化的二十代以后,构造中国S.mutans异种被证实稳定。aciduricity的重要差别在S.mutans标准紧张和标准紧张的抵抗是的勒克司变异的strain.The酸之间被观察比二紧张两个都显示了的勒克司变异的strain.The的强壮酸忍耐回答的能力。结论:S.mutans基因突变而产生之遗传的交换plasmid校正地被构造并且一S.mutans的勒克司否定的异种被构造,它能帮助推进在S.mutans的致病学习勒克司的角色。勒克司变异的紧张对酸忍耐回答的酸inactivation,而是能力更敏感仍然存在。
简介:WedescribetheGALT-Protdatabaseanditsrelatedweb-basedapplicationthathavebeendevelopedtocollectinformationaboutthestructuralandfunctionaleffectsofmutationsonthehumanenzymegalactose-1-phosphateuridyltransferase(GALT)involvedinthegeneticdiseasenamedgalactosemiatypeI.Besidesalistofmissensemutationsatgeneandproteinsequencelevels,GALT-ProtreportstheanalysisresultsofmutantGALTstructures.Inadditiontothestructuralinformationaboutthewild-typeenzyme,thedatabasealsoincludesstructuresofover100singlepointmutantssimulatedbymeansofacomputationalprocedure,andtheanalysistoeachmutantwasmadewithseveralbioinformaticsprogramsinordertoinvestigatetheeffectofthemutations.Theweb-basedinterfaceallowsqueryingofthedatabase,andseverallinksarealsoprovidedinordertoguaranteeahighintegrationwithotherresourcesalreadypresentontheweb.Moreover,thearchitectureofthedatabaseandthewebapplicationisflexibleandcanbeeasilyadaptedtostoredatarelatedtootherproteinswithpointmutations.GALT-Protisfreelyavailableathttp://bioinformatica.isa.cnr.it/GALT/.
简介:
简介:ThaijasminericeKDML105isconsumedaroundtheworld.BKOS,PKOSandTKOSarenewcultivarsproducedfromlow-energyionbeaminductioninKDML105.ThepurposeofthisstudyistocomparethemorphologyandanatomybetweenKDML105andthethreenewcultivars.Seedsofthefourcultivarsweregerminatedandgrowninpotsuntilfloweringphase.Theplants'organswereobservedandthelengthsofculms,ligules,leavesandpaniclesweremeasured.Leafsurfaceareawascalculatedandnumbersofroots,spikeletsandtillerswerecounted.BKOSandPKOShadsignificantlyshorterculmsthanKDML105andTKOS.ThelargestleafareawasfoundinKDML105followedbyTKOS,BKOSandPKOS,respectively.NumbersofrootsandtillersinBKOSandTKOSweresignificantlyfewerthanthoseinKDML105andPKOS.ThenumberofspikeletsperplantinBKOSwasthelowestamongallcultivars.Foranatomicalcomparison,crosssectionsofculmsandrootswereobserved.Allplantshadasimilararrangementoftissues,butthenumberandsizeofcellsweredifferent.Furthermore,longitudinalsectionsofculmsshowedthatthelengthsofepidermalandparenchymacellsweredirectlyrelatedwiththelengthoftheculm.Tocomparetheleaves,bothstomataandepidermalcellswerecountedandthelengthsoftheguardcellsweremeasured.ThelengthsofguardcellsofBKOSandPKOSwereshorter,butthestomataldensityandthestomatalindexweresignificantlygreaterthanthoseofKDML105.ForTKOS,thoughthelengthofguardcellswasshorterthanthatinKDML105,thedifferencewasnotsignificant.However,thestomataldensityandstomatalindexweresignificantlyhigherthanthoseinKDML105.