简介：Epicuticularwaxformstheoutermostprotectivebarrieroftheaerialsurfacesoflandplants,workinginconcertwithothercomponentsoftheplantcuticletopreventuncontrolledlossofwaterandtoprovideprotectionagainstanarrayofexternalenvironmentalstress.Inthisstudy,chemicallymutagenizedpopulationsofrice(OryzasativaL.)derivedfromapproximately4750M2familieswerescreenedforadhesionofwaterdropletsresultinginawetleaf/glossy(wlg)phenotype.Mutantswereidentifiedin11independently-derivedM2families.Scanningelectronmicroscopyanalysisconfirmedtheassociationofthewlgphenotypewithchangesintheepicuticularwaxcrystalsoftheseplants.Thephenotypesoffivemutants(7-17A,26.1,524.2,680.2,and843.1)wereconfirmedtobetheresultofsinglerecessivegenemutation.Evaluationofmutantsfrom3(6-1A,7-17A,and11-39A)of11M2familiesrevealedsignificantreductions(>50%)insurfacewaxcontentandincreasesincuticlemembranepermeability.
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