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简介：<正>AlthoughGuanMoyehasadoptedthenomdeplumeMoYan-meaning’don’tspeak’inChinese,itseemsanendlesscascadeofutteranceshasbeenemergingfromhispenorcomputer.Whateverthesubjectmatter,atorrentialflowofrich,unpredictableandoftenlaceratingwordsremainhistrademark.

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简介：在Poaceae的钙举起，translocation和累积的机制充分还没被理解。处理这个问题，我们在钙(Ca2+)的silico分析进行了染色体宽的比较级二庄稼种，米饭和蜀黍的transporter基因家庭。基因注解，在上游的行动cis元素的鉴定，种系发生的树构造和基因家庭印射的syntenic用几个生物信息学工具被执行。31Ca2+transporters的一个总数，从12个染色体在9上散布了，从米饭染色体被预言，当除了染色体10，从蜀黍预言的28Ca2+transporters在所有染色体上是分布式的时(Chr10)。有趣地，大多数Chr1和Chr3上的基因显示出在米饭和蜀黍之间的一种反的syntenic关系。这些transporter蛋白质的多重顺序排列和主题分析揭示了在二种之间的高保存。种系发生的树能很好在基因家庭之中识别隧道，ATPases和exchangers的子类。在里面silicocis规章的元素分析建议了与光，应力和荷尔蒙应答以及内乳特定、分裂组织特定的基因表示联系的多样的功能。进一步的实验被保证验证在里面预言的transporter基因家庭的silico分析并且阐明在各种各样的生物进程的Ca2+transporters的函数。

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简介：Sweetsorghum,theworld'sfifthmostimportantcerealafterrice,corn,wheatandbarley,isaveryattractivesourceforbiomassproduction.Thisrawmaterialcomplementssugarcaneproductionandisemergingastheidealfeedforanimalsandfortheproductionofthefirst-generationofbiofuels,suchasethanolandvalue-addedchemicals.Atpresent,mostofthestudiesaboutsweetsorghumfocusonutilization(suchasethanolproduction),andthestudyofsugeraccumulationinstemarerarely.

简介：Duringthepastyear,biophysicsgroupatInstituteofModernPhysics(IMP)obtainedlotsofachievementsintheresearchofheavy-ionmutationbreedingandproductionchainofsweetsorghum.Infundamentalresearchfield,amutantpopulationofArabidopsisthalianainducedbycarbonionbeamradiationwasestablishedinM2generation.Thetotalmutationratewas4.77%.Amutantmarked197#,whichhadfrostbite-like,palegreen,wrinkledandunevenleavesanddisplayedloosebractsandlatematuration,wasobtainedandreportedforthefirsttime.Generoughmappingresultsdemonstratedthatthereweretwomutationsitesinthe1stand4thchromosomeof197#mutant,indicatingheavyionradiationmightinducemorecomplicatedmutationsbeyondourcurrentrecognition.Thewholegenomeresequencingofthismutantisstillinprogress.Inaddition,high-yieldstrainsofmicrobeswhichhavepotentialvalueforcommercialapplication,suchasCorynebacteriumglutamicumandLacbobacillusthermophiles,werescreenedusingheavy-ionmutationtechnique.

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简介：Thehighgrowth-stimulatingeffectofplantextracthasurgedtheplantbiotechnologiststousenaturalsupplementsintheculturemediainsteadofsyntheticphytohormones.Weadvocatedtheeffectofsproutedsorghumextract(SSE)onemergence,invitroacclimatization,andgeneticfidelityincoleoptilederivedcallusofindicaricevarietyADT36.TheuseofSSEwithMurashigeSkoogmediumefficientlyacclimatizedtherootandshootapicalsystems.Ahighermatandseminalroots(3.4gbiomass)withanefficientshootprimordiumelongationwereobservedwithanincreaseintheconcentrationofSSE.SeedstreatedwithSSEmediumshowedhighergerminationandearliercoleoptilematurationabout48hcomparedtountreatedseeds,andtherewasahigherexpressionofeEF-1αwithanincreaseincoleoptilelength.B5mediumwaseffectiveoninducingembryogenicandnodularcallusfrom3-day-oldcoleoptilewith3.0mg/L2,4-dichlorophenoxyaceticacidandfurtherproliferatedeffectivelywith0.8mg/Lkinetinwithafreshweightof180mg.Highlysignificantregenerationwasobservedwithcombinationof2.5mg/L6-benzylaminopurineand3.0mg/Lα-naphthaleneaceticacid.Themetabolicandgeneticprofilesofinvitroanddirectlycultivatedplantswerethesame,examinedthroughFourier-transforminfraredspectroscopy,randomamplifiedpolymorphicDNA(RAPD),inter-simplesequencerepeat(ISSR)andR-ISSR(combinationofRAPDandISSR)markers,respectively,andthusconfirmingthesignificantefficacyoftheSSEincorporatedmedium.DisarmedT-DNAwastransformedtocoleoptilederivedcallusthroughAgrobacteriumtumefaciensLBA4404andconfirmedbyGUSassay.TheT-DNAintegrationwasconfirmedbyDNAblotanalysisusingDNAfromtransientGUS-expressedexplants.Thus,SSEcanbeusedasanaturalandorganicsupplementfororganogenesisandefficientacclimatizationsofshootandrootapicalmeristemsinregeneratedplants.