简介:摘要:广告时代,变译理论为广告翻译提供了新路径。研究在变译理论的基础上对苹果产品iPhone 12 Pro的广告语进行分析,并进一步探讨了广告翻译的基本原则,即主次性、叛逆性和本土化。
简介:AbstractBackground:There is growing evidence that 5-fluorouracil (5-FU) combined with therapeutic trauma can effectively induce skin repigmentation in vitiligo patients who are unresponsive to conventional treatments. Previous studies have mainly focused on identifying the antimitotic activity of 5-FU for the treatment of skin cancer, but few studies have investigated its extra-genotoxic actions favoring melanocyte recruitment.Methods:We utilized the full thickness excisional skin wound model in Dct-LacZ transgenic mice to dynamically assess the migration of melanocytes in the margins of wounds treated with or without 5-FU. The in-situ expression of CXCL12 was examined in the wound beds using immunofluorescence staining. Quantitative real-time polymerase chain reaction and Western blotting analyses were performed to detect the expression levels of CXCL12 mRNA and protein in primary mouse dermal fibroblasts treated with or without 5-FU. Transwell assays and fluorescein isothiocyanate (FITC)-phalloidin staining were used to observe cell migration and filamentous actin (F-actin) changes of melan-a murine melanocytes.Results:Whole mount and cryosection X-gal staining showed that the cell numbers of LacZ-positive melanocytes were much higher in the margins of dorsal and tail skin wounds treated with 5-FU compared with the controls. Meanwhile, CXCL12 immunostaining was significantly increased in the dermal compartment of wounds treated with 5-FU (control vs. 5-FU, 22.47 ± 8.85 vs. 44.69 ± 5.97, P < 0.05). Moreover, 5-FU significantly upregulated the expression levels of CXCL12 mRNA (control vs. 5-FU, 1.00 ± 0.08 vs. 1.54 ± 0.06, P < 0.05) and protein (control vs. 5-FU, 1.00 ± 0.06 vs. 2.93 ± 0.10, P < 0.05) in cultured fibroblasts. Inhibition of the CXCL12/CXCR4 axis suppressed melanocyte migration in vitro using a CXCL12 small interfering RNA (siRNA) or a CXCR4 antagonist (AMD3100).Conclusion:5-FU possesses a pro-pigmentary activity through activation of the CXCL12/CXCR4 axis to drive the chemotactic migration of melanocytes.
简介:摘要:论述了计算机辅助编程的步骤和Pro/E数控加工的基本流程,在Pro/E9.0环境下,实现了齿轮数控加工的全过程,并生成加工齿轮的NC代码。
简介:摘要:现有的CAD/CAM 系统主要是提供一个可以进行数控编程的人机交互式工具,并没有针对零件的特征信息提供一套计算机辅助的机制对数 加工的工艺设计进行指导,为了克服上述弱点,本文主要在车灯注塑模具的数控编程中汇集数控加工工艺专家智慧,并且充分利用这些数控加工工艺知识,所以在此基础上基于Pro/EEMXEM软件平台开发车灯模具数控编程模板。
简介:摘要:泰国的广告在泰国电影昙花一现之后依然保持着傲人的优秀,世界几大广告奖(克里奥、戛纳、莫比、纽约广告奖)每年都拿到手软,也顺便震惊了中国的社交网络,为此做案例分析。
简介:摘要目的探讨血清神经元特异性烯醇化酶(NSE)及胃泌素释放肽前体(Pro-GRP)在小细胞肺癌(SCLC)患者中疗效预测价值。方法回顾性研究2016年2月至2018年12月在复旦大学附属中山医院接受化疗的SCLC患者249例。分析治疗前后血清NSE、Pro-GRP值在各治疗反应组间的差异,分析其对近期疗效的预测价值。结果治疗前,缓解组及稳定组NSE均较进展组明显降低(P值均<0.05)。治疗后,缓解组及稳定组NSE均明显低于进展组(P值均<0.05);与稳定组比较,缓解组治疗后NSE更低(P值<0.05)。治疗后,缓解组Pro-GRP值均较进展组及稳定组明显降低(P值均<0.05)。治疗后,NSE值及Pro-GRP在缓解组13.3(11.4,16.9) μg/L和101.8(59.2,350.3) ng/L与未缓解组21.1(15.0,39.0) μg/L和507.0(82.6,2 164.0) ng/L间的差异均有统计学意义(P值均<0.001)。结论治疗SCLC患者中NSE可以预示早期疾病进展。治疗后NSE、Pro-GRP越低预示更好的疗效。NSE、Pro-GRP结合对近期疗效有较好的预测价值。
简介:摘要:目的 初步研制结合中医理论的多维度相结合的老年性肾阳虚型骨质疏松症患者的PRO评价量表。方法 基于中医基础理论,以肾阳虚型“骨痿”为基础,兼顾现代医学关于骨质疏松症的临床诊断特点指导下,检索文献收集条目池,筛选条目池形成量表初稿,经统计严格筛选出灵敏度和特异度高的条目,制成PRO量表。结论 统计分析建议剔除条目:使用SPSS 21.0软件统计分析,使用分布法、离散趋势法、t检验法等相关统计方法,得出最终条目。最终形成入选条目包括临床表证、社会关系、日常生活、治疗、心理等多维度相关条目,共计12条的肾阳虚型老年性骨质疏松症PRO量表。通过分半信度和Cronbach’sα系数对信度考评,具有良好的信度和效度,可以患者自我评价及临床评价的参考工具。
简介:摘要目的分析12例儿童Gitelman综合征(Gitelman syndrome,GS)的临床和实验室检查及SCL12A3基因变异情况。方法收集GS患儿临床资料,采用全外显组测序技术对患儿致病变异进行筛查,再通过Sanger测序对患儿及其父母进行验证。结果12例GS患儿的诊断年龄2岁8个月至15岁,男10例、女2例,6例因感染、5例因身材矮小、1例因下肢无力就医时发现血钾降低。12例患儿均检测出SLC12A3基因变异,其中复合杂合变异11例,纯合变异1例;共检测出19个致病等位基因,15个错义变异,2个移码变异,2个剪切区域变异。发现未报道的新变异位点3个,分别为c.578_582dupCCACC(p.Asn195Profs*109)、c.251C>T(p.Pro84Leu)、c.2843G>A(p.Trp948X)。结论儿童GS临床症状不典型,以年长儿多见,对偶然发现的低钾血症伴身材矮小或偏矮患儿,应注意GS的筛查。绝大多数GS患儿存在SLC12A3基因2个致病变异,以复合杂合变异为主,以p.Thr60Met最为常见。新变异的发现丰富了SLC12A3基因变异谱。