简介:包括16不育系的米饭isonuclear-alloplasmic线和他们的维护者线的四个集合被使用91份SSR教材学习原子染色体和他们的相对关系的基因差异分析。169等位基因的一个总数在16根线被检测,与53.85%的多态的loci和每1.8的地点的等位基因的一个平均数字的频率,并且平均基因差异是0.228。无菌的线分享了的isonuclear-alloplasmicmale的四个集合146相同等位基因,相应于86.39%全部的等位基因;同时,在测试材料之中有23不同等位基因,是13.61%全部的等位基因。平均,78.70%相同等位基因和全部的等位基因的21.30%不同等位基因在isonuclear-alloplasmic不育系和他们的维护者线之间被检测。Therewere53.85%相同等位基因和在homozygousallonucleus不育系之中的全部的等位基因的46.15%不同等位基因。指纹为某不育系和维护者线被建立。测试的所有材料基于簇分析在0.2遗传距离被划分成三个组。HuanongA和HuayuA(包括的Huanong乐队HuayuB)的八根线在第一个组,KezhenA的四根线(包括KezhenB)在在第三个组的Zhenshan97A(包括的Zhenshan97B)的第二个组,和四根线。为theisonuclear-alloplasmic不育系,在Y(Yegong)类型和WA之间的类似系数(野未成功)打字或在CW(Raoping野米饭)和WA之间打到达87-98%。
简介:Thepaperproposesaphysicalmodelforthemotionofthecontactlineandthegas-liquidinterface.Thelocalmotionofthecontactlineatthesolidwallisassumedandtheinterfacebetweengasandliquidistracedbyalevelfunction.Thefinitevolumemethodandstaggeredgridsareusedtosolvethegoverningequationnumerically.Themotionofthewatercolumninaverticalpipeiscomputedandtheresultsareingoodagreementwithexperimentaldata.
简介:Threeresidualheterozygouslines(RHLs)carryingheterozygoussegmentsintheintervalsRM587-RM225,RM204-RM6119andRM6119-RM402ontheshortarmofricechromosome6,respectively,wereselectedfromaricepopulationderivedfromanRHLfortheintervalRM587-RM402.Tenmaternalhomozygotes,10paternalhomozygotesand20heterozygoteswereselectedfromeachoftheF2populationsderivedfromthethreeRHLs.Thethreesetsofnearisogeniclines(NILs)weregrowntodetectthegrainyieldperplant,numberofpaniclesperplant,numberoffilledgrainsperpanicleand1000-grainweight.WithanalysisonthephenotypicdifferencesamongthethreegenotypegroupsineachNILsetandthoseamongoverlappingchromosomesegmentsubstitutionlines,threeQTLsfornumberoffilledgrainsperpanicleandtwoQTLsforgrainyieldperplantwereresolved.Theywerelocatedintheintervalsrangingfrom0.66Mbto2.49Mb.Theadditiveeffectwashigherthanthedominanceeffectateachlocus.ThealleleforincreasingthetraitvaluewasderivedfromthepaternalparentatqNFGP6-1,andfromthematernalallelesatotherQTLs.Basedonthepresentstudy,anapproachforconstructingnewgeneticresourcetofacilitatefinemappingofQTLsinricewasproposed.
简介:TheDNAfragmentsabout1600bpwereamplifiedusingrandomamplifiedpolymorphismDNA(RAPD)primerOPA12withthetemplatesofmitochondrialDNAofZhenshan97AandZhenshan97B,andweresequenced.ThenucleotidesequencesandlengthsofthefragmentsfromZhenshan97AandZhenshan97Bshowednodifference.Thepreciselengthofthefragmentwas1588bp.Sequencecharacterizedamplificationregion(SCAR)primerswerethendevelopedtodiscriminatethecytoplasmicmalesterile(CMS)linesandtheirmaintainerlines.Aspecific1588bpfragmentcouldbeamplifiedwithSCARprimers,CHI19F2/CHI19R2andCHI20F3/CHI23R3,inthemitochondrialDNAofZhenshan97A,butnotZhenshan97B.Furthermore,thespecificfragmentcouldbealsoamplifiedfromthetotalDNAfromgreenleaftissuesofZhenshan97AwithSCARprimers,butnotZhenshan97B.Withthecorrespondingprimers,thespecificfragmentcouldalsobeamplifiedfromthetotalDNAofgreenleavesofothertwoCMSlineswithwildabortivetypecytoplasm(CMS-WA),namelyZhenpinAandTianfengA,butnotintheirmaintainerlines.Moreover,usingtotalDNAastemplate,eachofthefourpairsofSCARprimerscouldalsobeusedtoamplifythe1588bpfragmentinCMS-ID(Indonesiapaddytype)lineⅡ-32A,butnotinII-32B,andthespecificfragmentwasamplifiedfromtheDNAofbothF1andF2seedlingsofShanyou63.Theresultsofdetectingthegeneticpurityofaman-mademixtureoftheseedsofZhenshan97AusingCHI20F3/CHI23R3werecompletelyconsistentwiththephenotypes.Takentogether,theseresultsindicatedthatthespecific1588bp-fragmentamplifiedbyCHI20F3/CHI23R3wastheuniqueamplificationproductsofCMSmitochondrialDNA,andcouldbeusedtodistinguishCMS-WAandCMS-IDlinesfromtheircorrespondingmaintainerlinesattheseedlingstage.
简介:Thispaperanalyzestheadvantagesanddisadvantagesoftwotechni-callinesforautomaticgroupgeneralizationofcontourlines.Theauthorsuggeststhatitispossibletogetfasterandbettergeneralizationresultsifwesimulatetheintelligenceofhumanexpertsinprogramdesigning,retrievegeomorphologicalstructuralinformationusingtheinputdataof2-Dcontourlinesandderiveandoutputthegeneralied2-Dresultsdirectly.
简介:IceformationintheharboursinarcticregionsuchasinFinlandisaprobleminwintertimes.Theairbubblersareoftenusedforcontrollingthegrowthoficeneartheharbourpierwalls.Thispapergivesanin-depthdescriptionoftheharbouriceproblemandtheapplicabilityofthebubblers.Anumericalmethodofflowandheat-transferisusedtopredicttheeffectivenessoftheairbubblersincontrollingtheiceaccumulationintheharbours.Empiricalmodelsofformattingandmeltingtheicearepresentedandusedinthenumericalsolutions.Itshowsthatthenumericalmethodcanrealisticallypredicttheice-meltingeffectoftheairbubblers.
简介:Objective:TodetectdifferentialproteinexpressioninmalignantandnormallivercelllinesinvitrousingtheSELDIProteinChipplatform,forinvestigatingthepathogenesisoflivercancer.Methods:Twocelllines,humannormallivercelllineL02andhepatomacelllineSMMC-7721wereculturedroutinely,harvestedingoodconditionandlysed.Afterquantification,thesupernatantofthelysatewastestedbyIMAC3(ImmobilizedMentalAffinityCapture)andWCX2(WeakCationExchange)chipsontheSELDI-TOF-MSProteinChipreader.Results:Proteinexpressiondifferedbetweenthemalignantandnormallivercelllines.Atotalof20differentiallyexpressedproteinswerefound,amongwhich,7werecapturedbytheIMAC3chipand14bytheWCX2chip.Peaksat5,419,7,979and11,265Dawerehigherandat8,103,8,492,10,160and11,304DalowerinSMMC-7721cellsbytheIMAC3chip;peaksat7,517,7,945and7,979Dawerehigherandat5,061,5,551,5,818,7,439,9,401,10,100,10,312,11,621,11,662,11,830and12,772DalowerinSMMC-7721cellsbytheWCX2chip.Interestingly,bothchipscapturedthe7,979Dapeak.Inaddition,the11,081DapeakcorrespondedpreciselywiththemolecularmassofthecalciumbindingproteinS100A10,whichmayparticipateintheformationoflivercancerinassociationwithp36.Conclusion:DetectingdifferentialproteinexpressioninmalignantandnormallivercelllinesusingtheSELDIProteinChipplatformwassimple,sensitiveandrepeatable.Theresultsweobtainedcanserveasabasisforinvestigatingthepathogenesisoflivercancerandaidthediscoveryofnewtherapeutictargets.
简介:I.IntroductionA31.2km110kVtransmissionlineisdesignedtooperateunder35kVwithconductormodelAC-185(aluminiumstrand--28x2.87mm~2,steelcore--7x2.50mm~2)ataverageoper-atingstressof24.2%,andoverheadgroundwiremodelC-50(steelstrand--7x2.97mm~2)ataverageoperatingstressof20.6%.Thewholelinehas130pole-towers,mostofwhichareofprestressedconcretedouble-polestructure,asshowninFig.1.The
简介:Inthelightofφ-mappingmethodandtopologicalcurrenttheory,theeffectofdisclinationlinesonthefreeenergydensityofnematicliquidcrystalsisstudied.ItispointedoutthatthetotalFrankfreeenergydensitycanbedividedintotwoparts.Oneisthedistortedenergydensityofdirectorfieldaroundthedisclinationlines.Theotheristhesaddle-splayenergydensity,whichisshowntobecentralizedatthedisclinationlinesandtobetopologicallyquantizedintheunitofkπ/2whentheJacobiandeterminantofthedirectorfielddoesnotvanishatthesingularitiesofthedirectorfield.ThetopologicalquantumnumbersaredeterminedbytheHopfindicesandBrouwerdegreesofthedirectorfieldatthedisclinationlines,i.e.,thedisclinationstrengthes.WhentheJacobiandeterminantvanishes,thegeneration,annihilation,intersection,splittingandmergingprocessesofthesaddle-splayenergydensityaredetailedintheneighborhoodsofthelimitpointsandbifurcationpoints,respectively.Itisshownthatthedisclinationlinewithhightopologicalquantumnumberisunstableandwillevolvetothelowtopologicalquantumnumberstatesthroughthesplittingprocess.
简介:Incontinuousannealinglines/hotgalvanizing,stripsareguidedbyupperandlowerrollsinanalternateupanddownverticalmotion.Thiskindofprocessconsistingofheating,soaking,andcoolingfurnaces(orzones)hasseveraladvantagesintermsofproductivityandproductquality.Mainproblemsencounteredincontinuousannealinglinesaremisguidingandformationofbuckling.Thestripbucklingcausesthestriptoberejectedand,intheworstcase,thestripbreaksandthecontinuousannealinglinesarecompelledtostop.Inthispapertheelastic-plasticmodelofthestriptravelingandthecorrespondingboundaryconditionwereconstructeddependantonthetheoryofmechanic.Simulationsareperformedintwosteps.First,ahomogeneouslongitudinaltensilestressisappliedatoneendofthestrip,theotherendofthestripandtherollarerestricted.Inthesecondstep,theappliedtensionremainsconstant.Auniformdisplacementisappliedtotheotherendofthestripandarotationtotheroll.Thetransversecompressivestress,thebucklingformationduringstriptravelingprocess,thelocationforthebucklingtakingplaceandthebucklingcriterionwereinvestigatedsystematically.Theresultshowsthatthetransversecompressivestressincreaseswiththeincreaseofthetensilestressandthecrownofthetaperedroll.Becausethecontactareabetweenthetaperedrollandthestripincreasewiththedecreaseofthecrown,thetensilestressdistributionismorehomogeneousinthetransversedirectionofthestrip.Anadditionaltransversecompressivestressiscreatedandabucklingisalsoobservedduetothefrictionduringthestripmotioninthesecondstep.Oncethebucklingisappearance,itisveryhardtoberemovedonlythroughdecreasingtheappliedlongitudinaltension,becausethestripwrinklestillkeepshighlevelduetotheeffectofthebuckledstrip,thewrinklewouldevolveintobucklingeventually.Thebucklingformationprocesspredictedbythefiniteelementmodelisverysimilartothose
简介:Asemi-analyticalmethodintimedomainispresentedforanalysisofthetransientresponseofnonuniformtransmissionlines.Inthismethod,thetelegraphequationsintimedomainisdifferencedinspacedomainfirst,andistransformedintoasetoffirst-orderdifferentialequationsofvoltageandcurrentwithrespecttotime.Byintegratingthesedifferentialequationswithrespecttotime,andprecisecomputation,thesolutionofthesedifferentialequationscanbeobtained.Thismethodcansolvethetransientresponseofvariouskindsoftransmissionlineswitharbitraryterminalnetworks.Particularly,itcananalyzethenonuniformlineswithinitialconditions,forwhichthereisnoexistingeffectivemethodtoanalyzethetimeresponsesofar.Theresultsobtainedwiththismethodarestableandaccurate.Twoexamplesaregiventoillustratetheapplicationofthismethod.
简介:Thepartialchargesimulationmethodispresentedtosolvethecharacteristicimpedanceofthetransmissionlineofspecificcrosssectionwithanoffsetinnerconductor.Thismethodhasahigheraccuracyduetotheaccuratesatisfactionoftheboundaryconditionontheouterconductor.ThecombinedmethodoftheGausseliminationandoptimizationisusedtosolvetheequationofchargesimulation,anditisaneffectivemethodforincreasingtheaccuracyandassuringtheconvergence.TheGreen’sfunctionsoffivetransmissionlines(i.e,withcircular,elliptic,rectangular,troughandslabconductor)aregiven.
简介:在技术集中的市场,让公司开发长期的多重产生生产线而不是释放连续单个产品是普通策略。尽管这策略比顺序介绍单个产品代更有利,它能也导致内部产品的线cannibalization。多重产生的生产线的Cannibalization是需要在计划舞台以便支撑长期的盈利的早生产线被考虑的一个复杂问题。在这份报纸,我们建议能在一条多重产生的生产线以内模仿潜在的cannibalization情形的一个基于代理人的模型。我们把一条多重产生的生产线(MGPL)看作在MGPL的产品产生各在市场上基于移动随着时间的过去调整它的出售价格的复杂适应系统需求。建议模型提供各种各样的定价策略怎么影响MGPL和罐头的全面生命周期盈利的卓见被用来在在计划阶段的早生产线开发适当动态定价策略帮助公司。