简介:Smallubiquitin-likemodifier(SUMO)-conjugatingenzymesareinvolvedinpost-translationalregulatoryprocessesineukaryotes,includingtheconjugationofSUMOpeptidestoproteinsubstrate(SUMOylation).SUMOylationplaysanimportantroleinimprovingplanttolerancetoabioticstresssuchassalt,drought,heatandcold.Herein,wereportedtheisolationofOsSCE1(LOC_Os10g39120)geneencodingaSUMO-conjugatingenzymefromrice(Oryzasativacv.Nipponbare)anditsfunctionalvalidationinresponsetodroughtstress.TheE2enzyme,OsSCE1,isoneofthreekeyenzymesinvolvedintheconjugationofSUMOtoitstargetproteins.ActivatedSUMOistransferredtothecysteineofanE2enzymeandthentothetargetlysineresidueofthesubstrate,withorwithoutthehelpofanE3SUMOligase.ExpressionofOsSCE1wasstronglyinducedbypolyethyleneglycol6000(PEG6000)treatment,whichsuggestedOsSCE1maybeinvolvedinthedroughtstressresponse.OverexpressionofOsSCE1(OsSCE1-OX)inNipponbarereducedthetolerancetodroughtstress.Conversely,thedroughttolerancewasslightlyimprovedbytheknockdownofOsSCE1(OsSCE1-KD).TheseresultswerefurthersupportedbymeasurementofprolinecontentinOsSCE1-OXandOsSCE1-KDtransgeniclinesunderinduceddroughtstress,whichshowedOsSCE1-KDtransgeniclinesaccumulatedhigherprolinecontentthanthewildtype,whereasOsSCE1-OXlinehadlowerprolinecontentthanthewildtype.ThesefindingssuggestedOsSCE1mayplayaroleasanegativeregulatorinresponsetodroughtstressinrice.
简介:淀粉,直链淀粉,蔗糖内容和在在开花以后的shading处理下面的淀粉合成酶的活动的动态变化作为材料用二米饭变化IR72(indica)和Nipponbare(装饰用的梨树)被学习。当仅仅有点改变的自动数据处理葡萄糖pyrophosphorylase(ADPGPPase)活动,可溶性淀粉synthase活动和小粒绑了淀粉synthaseactivity时,在shading处理,thest拱门,直链淀粉和蔗糖下面,内容减少了减少,可溶性淀粉分叉酶(SSBE,Q酶)活动和小粒界限淀粉分叉酶(GBSBE,Q酶)增加的活动,和淀粉初上舞台的人ranching酶(DBE,R酶)活动与变化变化了。关联分析证明淀粉内容的变化断然并且显著地在弱光与蔗糖内容的变化被相关。ADPGPPase活动和SSBE活动断然并且显著地与淀粉累积率被相关。淀粉synthase活动的衰落与淀粉内容的减少有关,淀粉分叉酶的活动的增加在弱光下面在直链淀粉的比率的减少起了一个重要作用到全部的淀粉,这被暗示。
简介:Effectsofshadingatthefloweringandgrainfillingstagesonosmoticregulationsubstancecontentandprotectiveenzymeactivitiesintheleavesofdifferentricegenotypes(e.g.,IIyou498,Gangyou188,Dexiang4103,Gangyou527andChuanxiang9838)wereinvestigated.TheresultsshowedthatthemalondialdehydecontentofGangyou188significantlyincreasedundershading,whereasthoseofIIyou498,Dexiang4103,Gangyou527andChuanxiang9838showednosignificantdifferencescomparedtothecontrol.ThesolublesugarcontentsignificantlydecreasedinIIyou498,Gangyou188,Dexiang4103andChuanxiang9838,whereasitdidnotsignificantlyincreaseinGangyou527undertheweaklightconditions(shading).Moreover,thesolubleproteincontentintheleavesofdifferentgenotypestendedtodecreaseundershading.Further,theprotectiveenzymeactivitiesintheleavesvariedindifferentricegenotypesundertheshadingtreatment.Basedontheosmoticregulationsubstancecontentandtheprotectiveenzymeactivitiesintheleavesofdifferentricegenotypes,itwasconcludedthatGangyou188andGangyou527hadstrongabilitiestoadapttothelowlightconditions.Inaddition,themechanismofdamagetoriceleavesindifferentgenotypesundershadingwasdiscussed.
简介:在flowering舞台被使用二装饰用的梨树米饭变化Koshihikari和Sasanishiki在天赋和高温下面学习以后,蔗糖,fructose,蔗糖synthase的葡萄糖内容和差别迟钝,vacuolar转化酶,和细胞壁的动态变化在米饭谷物绑了转化酶。在米饭谷物,蔗糖synthase活动比转化酶的高,它显著地与淀粉累积率被相关,显示蔗糖synthase起了在蔗糖降级和淀粉合成的一个重要作用。在高温下面,没有fructose和葡萄糖的任何增加的谷物蔗糖内容的重要增加满足,建议高温处理提高了蔗糖累积,当减少时在米饭谷物的蔗糖降级。与控制植物相比,在蔗糖synthase,vacuolar转化酶,和有高温的细胞壁界限转化酶的活动的减少对待显示的植物蔗糖降级的减速与在蔗糖synthase和转化酶的活动的减少有关。
简介:Cd忍耐和米饭幼苗的translocation上的H2O2预告的处理的效果用在Cd忍耐不同的二米饭栽培变种(N07-6和N07-63)被学习。malondialdehyde(MDA)的内容,减少的谷胱甘肽(GSH),非蛋白质thiols(NPT),phytochelatins(PC)和谷胱甘肽S-transferase(GST)的活动在暴露于各种各样的处理的二栽培变种之间被比较。结果证明50mol/LCd暴露显著地禁止了米饭生长,提高了GSH,NPT,PC和MDA的生产,并且增加了GST的活动,并且二栽培变种之间有重要差别。更多的Cd被搬运进N07-6的射击。H2O2预告的处理由进一步在根增加GSH,NPT和PC内容,以及GST活动减轻了Cd毒性。在N07-63的这些参数的增加度比在N07-6的那些高,建议N07-63的忍耐比N07-6更显著地被提高。氢过氧化物把Cdtranslocation归结为米饭射击,但是不同地在根影响了Cd内容。从上述结果,在到在二栽培变种之间的H2O2预告的处理的Cddetoxification和反应有显著差别,这可以被推测。
简介:到怀有基因Pi-d2从pCB6.3kb,pCB5.3kb和pZH01-2.72kb的三不同表示向量转变了的米饭强风抵抗的转基因的米饭线的米饭强风的抵抗被分析。有Pi-d2基因的九根先进产生的转基因的米饭线显示了各种各样的抵抗到39米饭强风紧张,并且最高疾病抵抗的频率到达了91.7%。有Pi-d2基因的四根早产生的同型结合的转基因的线展出了抵抗到58米饭强风紧张中的超过81.5%个,显示出宽光谱的抵抗的特征。当在文化媒介的粗略的毒素的集中增加了,米饭强风真菌的粗略的毒素选择的转基因的胚胎的calli证明从转基因的米饭植物的不成熟的胚胎的胼胝正式就职率减少了。当粗略的毒素的集中到达了40%时,从转基因的线的不成熟的胚胎的胼胝正式就职率是49.3%,并且受体控制的是5%。在正式就职下面的地里的转基因的大米线的颈强风的疾病发生是0%~50%,显示转基因的大米线的大米强风抵抗比受体控制的高得多。
简介:Tiller角度,一个很必要的农学的特点,在米饭繁殖是重要的,特别在植物类型繁殖。控制2的一个tiller角度(tac2)异种被乙醇甲烷磺酸盐mutagenesis从restorer线Jinhui10获得。tac2异种在幼苗阶段和显著地在tillering阶段增加的tiller角度显示了正常显型。初步的生理的研究显示异种对GA敏感。因此,TAC2和TAC1可能以一样的方法控制tiller角度,这被推测。基因分析证明变异的特点被主要后退的基因控制并且位于用SSR标记的染色体9。在TAC2和它的最近的标记RM3320和RM201之间的基因距离分别地是19.2厘米和16.7厘米。
简介:Byusing304recombinantinbredlinesderivedfromindicaricecrossZhong156/Gumei2,alinkagemapconsistingof177markerlociandcovering12ricechromosomeswasconstructedandemployedformappinggenesconferringblastresistanceinrice.GenomiclocationofgenePi25(t)conferringneckblastresistancetotheChineseisolate92-183(raceZC15)wasverifiedtobelocatedbetweenmarkersA7andRG456onchromosome6,withgeneticdistancesof1.7cMand1.5cMtoA7andRG456,respectively.LeafblastresistanceofGumei2tothePhilippineisolateCa89(lineage4)wasfoundtobecontrolledbyasinglegene.ThegenetentativelydesignatedasPi26(t)waslocatedbetweenmakersB10andR674onchromosome6,withgeneticdistancesof5.7cMand25.8cMtoB10andR674respectively.ResistantallelesatbothgenelociwerederivedfromGumei2,indicatinganexistenceofresistancegeneclusterinGumei2.
简介:到在米饭germplasm91-1A2的米饭胆量小蚊的抵抗被识别并且遗传上分析了。米饭人口的F1s从作为一个男父母与米饭材料Jinggui,TN1,W1263(Gm1),IET2911(Gm2),BG404-1(gm3),OB677(Gm4),ARC5984(Gm5)和Duokang1(Gm6)交叉的91-1A2被导出。到米饭胆量小蚊的所有父母线和F1,BC1F1和F2人口的抵抗被识别。结果证明91-1A2和所有F1s对中国米饭胆量小蚊遗传因子型IV抵抗。到在BC1F1和F2的易受影响的抵抗植物的分离比率被X2测试与1:3和9:7规则给予,建议到中国米饭胆量小蚊遗传因子型IV的91-1A2的抵抗被是新抵抗基因的二主导的基因控制,对已知的米饭胆量小蚊抵抗基因非突变而产生之遗传因子。
简介:Thetransgenicrice,Zhongda2,whichwasgeneticallymodifiedfromanindicaricelineZhuxianBbyricechitinasegene(RC24),hadhighresistancetoricesheathblight(Rhizoctoniasolam)inlaboratoryandatwo-yearfieldexperiment.ThepathogencouldinvadesheathofZhongda2andinducesymptomsofthedisease.NodifferencewasnotedintimeofpenetrationorincubationperiodbetweenZhongda2andnon-transgenicricecontrol,ZhuxianB,butthehyphaelysatecouldbeobservedeadierthancontrol.Itsresistanceexpressedastoinhibitthegrowthofmyceliuminhosttissue.F1sfromZhongda2(♂)crossedwithotherfivenon-transgenicricelinesshowedhigherresistancethandonornon-transgenicparents,buttheresistancewasdifferentalongwiththedifferentmaternalparents.
简介:Bacterialleafblightofrice(BLB),causedbyXanthomonasoryzaepv.oryzae,isoneofthemostdestructivediseasesinAsianricefields.Ahigh-qualityricevariety,LT2,wasusedastherecipientparent.IRBB21,whichcarriestheXa21gene,wasusedasthedonorparent.TheresistancegeneXa21wasintroducedintoLT2bymarker-assistedbackcrossing.ThreeXooraceswereusedtoinoculatetheimprovedlinesfollowingtheclippingmethod.ElevenBC3F3linescarryingXa21wereobtainedbasedonmolecularmarkersandagronomicperformance.The11linesweretheninoculatedwiththethreeXooraces.Allthe11improvedlinesshowedbetterresistancetoBLBthantherecipientparentLT2.BasedonthelevelofresistancetoBLBandtheiragronomicperformance,fivelines(BC3F35.1.5.1,BC3F35.1.5.12,BC3F38.5.6.44,BC3F39.5.4.1andBC3F39.5.4.23)wereselectedasthemostpromisingforcommercialrelease.Theseimprovedlinescouldcontributetoriceproductionintermsoffoodsecurity.
简介:布朗planthopper(Nilaparvatalugens圣?l)大多数损坏害虫之一在米饭正在引起hopper灼伤,并且从而减少生产率并且另外产品的质量。控制这个害虫的有效管理策略是到本地米饭栽培变种的理想的基因的鉴定和转移。为开发抵抗栽培变种的最重要的途径是标记的鉴定,它能在更持久的抵抗遗传型的帮助标记的选择帮助。易受影响的父母IR50和抵抗父母Ptb33,和他们的F2人口是为有随机的放大多态的DNA的抵抗基因的鉴定的使用的inbulkedsegregant分析标记(RAPD)教材。教材OPC7和OPAG14证明主导、易受影响的特定的banding模式那么叫了co主导的标记。而且,OPC7697和OPAG14680给抵抗特定的乐队看了并且因此在联合分阶段执行,而OPC7846和OPAG14650给易受影响的特定的genotypic乐队看了inbulkedsegregant分析。因此,联合阶段标记,OPC7697和OPAG14680,被认为在在庄稼改进的米饭遗传型的帮助标记的选择更有用。