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简介：新奇有势力朊酶，Urechisunicinctusfibrinolytic酶(UFE)，第一被发现。UFE的酶的性质进一步被调查。作为低分子的集体蛋白质，UFE看起来对热和氢指数很稳定。当温度在50℃下面时，残余酶活动仍然保持几乎未改变，但是当温度被提起到60℃时，残余酶活动开始很快减少了。UFE在从3～12的pH的范围是相当稳定的，特别在稍微碱的pH。Mn~(2+)，Cu~(2+)和Fe~(2+)离子是UFE的使活跃之物，当Fe~(3+)和Ag~+离子是UFE的禁止者时。沿着离子可能调整的withFe~(3+)的Fe~(2+)离子在vivo的UFE活动。UFE的最佳氢指数和温度分别地是大约第8a50℃。这酶的另外的特征也被学习。当UFE被申请时，系统的研究结果是重要的医药、工业的目的。

简介：Objective:ToevaluatethevalueofenhancedluminescenceenzymeimmunoassayinthediagnosisofNeisseriagonorrhea(NG)infection.Methods:Anti-catalaseantibodyforNeisseriagonorrheaecombinedwithenhancedluminescenceenzymeimmunoassaywereusedtotestforN.gonorrhea.Results:Aminimumof1×10^4/CFUofGCingenitaltractsecretionsorurinecouldbedetectedwiththetechniqueofluminescenceenzymeimmunoassay.Conclusion:TheenhancedluminescenceenzymeimmunoassayhastheadvantageofhighsensitivityandspecificityfordiagnosingNGfromgenitourinarytractsecretionandurine.

简介：TheeffectsofLaonsomehydrolyticenzymeactivitiesinredsoilwerestudiedinincubationandpetcultureexperiments.Intheincubationexperiment,Laslightlystimulatestheactivitiesofureaseandacidicphosphataseinsoilandstronglystimulatessucraseactivityinsoil.Inthepetcultureexperiment,Lastimulatestheactivitiesofurease,acidicphosphataseandsucrasetodifferentdegrees.Thestimulativeeffectsofrareearthelements(REE)onhydrolyticenzymeactivitiesinsoilmayresultinincreasingyieldofeivps.

简介：Cancergenomicsisarapidlygrowingdisciplineinwhichthegeneticmolecularbasisofmalignancyisstudiedatthescaleofwholegenomes.Whilethedisciplinehasbeensuccessfulwithrespecttoidentifyingspecificoncogenesandtumorsuppressorsinvolvedinoncogenesis,itisalsochallengingourapproachtomanagingpatientssufferingfromthisdeadlydisease.Specificallycancergenomicsisdrivingclinicaloncologytotakeamoremolecularapproachtodiagnosis,prognostication,andtreatmentselection.Wereviewhererecentworkundertakenincancergenomicswithanemphasisontranslationofgenomicfindings.Finally,wediscussscientificchallengesandresearchopportunitiesemergingfromfindingsderivedthroughanalysisoftumorswithhigh-depthsequencing.

简介：ObjectiveThestudywillexploreeffectsoftheautoantibodiesagainstAT1receptorandangiotensinⅡontherefractoryhypertension.MethodsSeventy-sevenpatients(46menand31women)withessentialhypertensionweredividedintogroupsofrefractoryhypertension(RH)andhypertension(HT)accordingtothe1999WHO-ISHGuidelinesfortheManagementofHypertension.Fortynormotensives(22men)wererecruitedascontrols.Themeanagewas54.3±13yearsoldinRHgroup,53.5±9yearsoldinHTgroupand51.2±11.9yearsoldinnormotensives(NT)group.Themeanbloodpressurewas154.2±9.4/98.4±8.2mmHginRHgroupand130.1±7.6/80.5±6.7mmHginHTgroupaftercombinationdrugtherapyofhypertensionfor4weeks.BloodpressureinNTgroupwas120.8±11.7/76.4±7.2mmHg.Theepitopeofthe2ndextracellularloopsofAT1receptorwassynthesizedandusedasantigenstoscreentheautoantibodiesbyELISA.Plasmaangiotensin(Ang)IIwereexaminedbyaradioimmunoassay.ResultsT

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简介：Polybenzylglutamateasoneofpolyα-aminoacidsdisplayingexcellentbiocompatibilityandbiodegradabi-lityiscapableofreactingwithhydroxyalkyl-aminebyaminolysisandchangingthebenzylglutamatetohydro-xyalkylglutamine,whichpropertiescanobviouslybeimproved.

简介：Theaimofthisarticleistostudytheeffectivebehaviorofthesolutionofanonlinearproblemarisinginthemodellingofenzymecatalyzedreactionsthroughtheexteriorofadomaincontainingperiodicallydistributedreactivesolidobstacles,withperiodε.Theasymptoticbehaviorofthesolutionofsuchaproblemisgovernedbyanewellipticboundary-valueproblem,withanextrazero-ordertermthatcapturestheeffectoftheenzymaticreactions.

简介：一个entomopathogenic细菌，Xenorhabdusnematophila，被知道了由禁止联系免疫者的phospholipaseA2(PLA2)导致目标昆虫的重要免疫力的抑制，它随后关掉在在昆虫的有免疫力的调停是批评的eicosanoids的生合成。一些代谢物从细菌的文化肉汤发源被识别了并且包括benzylideneacetone，脯氨酸酷氨酸和acetylatedphenylalanine-glycine-valine，它被知道禁止从血球和胖身体提取的PLA2的酶活动。这研究在甜菜粟蚕的消化PLA2上测试了他们的效果，Spodopteraexigua。年轻幼虫喂了三代谢物的不同集中在100g/mL下面甚至在剂量在幼虫的开发导致了重要不利效果。特别地，他们在摄取的食物的消化效率导致了重要减小。所有三代谢物显著地禁止了在一个低micromolar范围从第五个中间形态幼虫的midgut腔提取的消化PLA2的催化活动。这些结果建议S的消化PLA2上的三细菌的代谢物的禁止的活动。exiguamidgut可以解释一些他们的口头的有毒的效果。

简介：Theoysters(Saccostreacuculiata)werecollectedfromfourstationsaroundXiamenIs-land(LunduPort,XinglinBay,Tong'anBay,Huangcuo).Therelationbetweenthelevelofpetroleumhydrocarboninwholetissueandthecontentsofglutathione(GSH),theactivityofselenium-dependentglutathioneperoxidase(Se-GPx)andglutathioneS-transferase(GST)indigestiveglandandgillwereinvestigated.Theresultsshowed:(1)Thecontentsofpetroleumhydrocarboninoystercollect-edfromfourstations(LunduPort,XinglinBay,Tong'anBay,Huangcuo)were380.68,112.34,27.31,20.37μg/gwetweight,respectively;(2)theactivityofSe-GPxandGSTindigestiveglandwaslowerthanthatingill,andthecontentofGSHseemedreversibly;(3)amongthefourstations,bothSe-GPxandGSTactivityofdigestiveglandandgillinSaccostreacuculiatasampledfromthefourstationsshowedagoodcorrelationwithwholetissuepetroleumhydrocarbon,couldbeasbiomarkersofseaoilpollution.

简介：很少信息都不在土壤酶活动的潜力上存在，它是敏感的玷污性质和管理，为在集水规模的沉积来源的描述。这研究的目的是为沉积在Hiv集水(55km2)采指纹作为tracer探索并且评估酶活动，伊朗。因此，四酶从水库检查水坝(沉积水池)从42个不同采样地点，以及从12件沉积样品被测量，盖住三个沉积来源区域(牧场/表面侵蚀，果园/表面侵蚀，和streambank侵蚀)。结果显示那，在向后的模式判别式分析，-glucosidase和脱氢酶之上基于，允许正确地要分到他们的来源区域的超过95%样品。这些酶作为输入数据被选择让一个混合模型决定取样的沉积来源的相对贡献。从在学习区域的牧场，果园和streambank来源的吝啬的贡献分别地作为11.3%，15.1%和73.7%被估计。用geochemicaltracers，从牧场的吝啬的贡献，果园和streambank，来源分别地作为14.1%，9.5%和74.8%被估计。联合生物化学并且geochemicaltracers，类似的价值被获得(18.7%，10.7%和70.7%，分别地)。我们的结果显示那项土壤酶活动允许沉积来源的好描述，和罐头提供一个互补工具给当前存在的沉积采指纹途径。然而，方法应该也在另外的区域被测试。

简介：ObjectivesTostudythechangesofnitricoxide,angiotensinⅡandsuperoxideanioninrenalarteryhypertensionpathogenesis.MethodsMaleWistarratsweighing256-285gweredividedinto5groupsrandomly,10ratsofeachgroup.Controlgroup:falseoperationwasmadeandroutinedietwasgiven;Ligaturegroup:leftrenalarterywasligatureduncompletelyandroutinedietwasgiven;Ligature+Losartangroup:leftrenalarterywasligatureduncompletelyandLosartan20mg·kg^-1·d^-1wasaddedinthedrinkingwater;Ligature+L-Arggroup:leftrenalarterywasligatureduncompletelyandL-Arg2g·kg^-1·d^-1wasaddedinthedrinkingwater;Ligature+L-Arg+Losartangroup:leftrenalarterywasligatureduncompletelyandL-Arg2g·kg^-1·d^-1andLosartan20mg·kg^-1·d^-1wasaddedinthedrinkingwater.Bloodpressureandheartrateweremeasuredbeforeandattheendoftheexperiment.Oneweekafterligature,bloodwasdrawntodetermineangiotensinⅡ,cGMP,nitricoxide,nitricoxidesynthase(NOS),O2^-,superoxidedismutase(SOD).ResultsSystolicbloodpressurewashigherinligaturegroupthanthatincontrolgroup(p<0.05),systolicbloodpressurewasmuchlowerinligature+Losartangroupthanthatinligaturegroup.Heartratedidnotchangesignificantlyafterexperiment(p>0.05).AngⅡwashigherinligaturegroupthanthatincontrolgroup,evenmuchhigherinligature+Losartangroup(p<0.01).TherewasnodifferenceofcGMPineachgroup(p>0.05).TheconcentrationofNOwaslowerinligaturegroup(p<0.05),NOwashigherinligature+L-Arg+Losartangroupthanthatinligaturegroup(p<0.05).O2^-washigherinligaturegroupandligature+L-Arggroupthanthatincontrolgroup(p<0.05),O2^-waslowerinligature+Losartangroupthanthatinligaturegroup(p<0.05).ThelevelofSODwaslowerinligaturegroupthanthatincontrolgroup(p<0.05),higherinligature+L-Arggroupandligature+L-Arg+Losartangroupthanthatinligature

简介：backgroundIt'sestablishedthatAngiotensinⅡanditsreceptorsareinvolvedinintimalhyperplasiaafterballooninjuryandstentrestenosis.Recentevidencealsosuggeststhatstatinshavesomeanti-intimalhyperplasiaeffects.Inthisstudy,theeffectofRosuvastatinonexpressionofangiotensinⅡreceptorsinrataorticendotheliumafterballooninjuryisthereforeinvestigated.MethodsAll52WistarKyotoratswereestablishedtoaortainjurymodelsby2Fballooncatheter,thenwererandomlydividedintoshamoperationgroup,aortainjurygroupandRosuvastatin-treatmentgroup.After14days,theaorticspecimensoftheanimalswereharvestedandperformedimmunohistochemistryanddeterminationofmolecularbiology.ResultsTheresultsshowedthat(i)The14days-ballooninjuryinducedobviousintimathickening(P<0.01),however,thephenomenonwasreducedby14days-treatmentwithRosuvastatin(P<0.01).(ii)TheexpressionsofangiotentionⅡtypeⅠ(AT1)andtypeⅡ(AT2)receptormRNAandproteinweremarkedlyup-regulatedbytheballooninjury(P<0.01),after14days-treatmentwithRosuvastatin,theexpressionofAT1receptormRNAanditsproteinwasdecreased(P<0.01),buttheexpressionofAT2receptormRNAanditsproteinwasfurtherincreased(P<0.05).ConclusionInthisstudy,weobservedthattheballooninjuryinduced-intimathickeningwasreducedbyRosuvastatininrats,whichmightbelinkedwiththeregulationofexpressionofangiotensinⅡreceptors.

简介：SelectivelyconvertingCOandH2togasolineproduct(isoparaffinandolefin)inonestepstillremainsagreatchallenge.WedemonstrateeffectiveH-USYzeolitesupportednano-cobaltbifunctionalcatalystsforthiscatalyticreaction,whicharepreparedbythenovelphysicalsputteringprocess.Particlesofthesputteredcobaltexistinnano-levelandarewell-dispersedonacidUSYzeolite.Easyactivationoftheloadednano-cobaltisalsoachievedinalow-temperaturehydrogenreductionatmosphere.Inthetandemcatalyticreaction,thesputteredbifunctionalCo/USYcatalystexhibitsamuchhigherCOconversionandhigherisoparaffinselectivitythantheconventionalimpregnatedone.ComparedwithH-Mor,H-Betaandotherzeolitessupportedcatalysts,H-USYzeolitesupportedcobaltcatalystshowstheclearestpromotionaleffectontheactivityofFischerTropschsynthesis.Thedescribedsynthesishereinprovidesanewpathwaytosolvetheproblemcausedbythestrongmetal-supportinteraction(MSI)inheterogeneouscatalysis.

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简介：Thebiologytreatmentofcellulasesorhemicellulasesisusedinthispaper.Theeffectsofcellulasesandhemicellulasesonsurfacewettabilityandgluingpropertyofpoplarandwheatstrawwereinvestigated.Itwasfoundthatthesurfacecontactanglesofpoplarandwheatstrawwerereducedandthesurfacefreeenergieswereincreasedaftertreatmentbytheseenzymes.Thegluingstrengthsofpopularandwheatstrawwereenhancedwiththeincreasesofthedosageofenzymesandthetimeofenzymatictreatment.

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