简介：在这研究，我们调查了白果树biloba摘录(GBE)和phosphodiesterase类型的联合的效果5(PDE-5)阴茎海绵体和下士的钾隧道活动的肌肉发达的音调上的禁止者弄平肌肉房间。从雄的新西兰白兔子的阴茎海绵体的脱衣为等轴的紧张研究在器官洗澡被装。在有1×的收缩以后；10−5 moll−1新肾上素，GBE(0.01-1 ；mgml−1)和mirodenafil(0.01-100 ；nmoll−1)一起被增加进机关洗澡。在electrophysiological研究，整个房间的水流被常规补丁夹钳技术在人的阴茎海绵体的有教养的光滑的肌肉房间记录。阴茎海绵体以一种剂量依赖者方式响应GBE被放松(从0.64%；±；8.35%；在0.01 ；mgml−1到52.28%；±；11.42%；在1 ；mgml−1)。在有0.03 的预告的处理以后；GBE的mgml−1，mirodenafil的弛缓的效果根本被增加集中。在tetraethylammonium(茶)以后(1 ；mmoll−1)管理，增加的效果被禁止(P<；0.01)。GBE的细胞外的管理增加了在一个剂量依赖者的外面的水流塑造的整个房间的K+。外面的水流的增加被1 禁止；mmoll−1茶。这些结果建议GBE能甚至在最低限度地有效的剂量增加mirodenafil的弛缓的力量。通过钾隧道的K+流动可能是涉及这synergistic松驰的机制之一。

简介：AbstractBackground:The effectiveness of bronchial thermoplasty (BT) has been reported in patients with severe asthma. This study compared the effects of BT and cryoballoon ablation (CBA) therapy on the airway smooth muscle (ASM).Methods:Eight healthy male beagle dogs were included in this experiment. In the preliminary experiment, one dog received BT treatment for both lower lobe bronchus, another dog received CBA treatment for 7 s on the upper and lower lobe of right bronchus, and 30 s on the left upper and lower lobe. The treatments were performed twice at an interval of 1 month. In subsequent experiments, the right lower lobe bronchus was treated with BT, and the left lower lobe bronchus was treated with CBA. The effects of treatment were observed after 1 (n = 3) month and 6 months (n = 3). Hematoxylin-eosin staining, Masson trichrome staining, and immunohistochemical staining were used to compare the effects of BT and CBA therapy on the ASM thickness, collagen fibers synthesis, and M3 receptor expression after treatment. One-way analysis of variance with Dunnett post hoc test was used to analyze the differences among groups.Results:In the preliminary experiment, the ASM ablation effect of 30-s CBA was equivalent to that of 7-s CBA (ASM thickness: 30.52 ± 7.75 μm vs. 17.57 ± 15.20 μm, P = 0.128), but the bronchial mucociliary epithelium did not recover, and large numbers of inflammatory cells had infiltrated the mucosal epithelium at 1-month post-CBA with 30-s freezing. Therefore, we chose 7 s as the CBA treatment time in our follow-up experiments. Compared with the control group (35.81 ± 11.02 μm), BT group and CBA group (13.41 ± 4.40 μm and 4.81 ± 4.44 μm, respectively) had significantly decreased ASM thickness after 1 month (P < 0.001). Furthermore, the ASM thickness was significantly lower in the 1-month post-CBA group than in the 1-month post-BT group (P = 0.015). There was no significant difference in ASM thickness between the BT and CBA groups after six months (9.92 ± 4.42 μm vs. 7.41 ± 7.20 μm, P = 0.540). Compared with the control group (0.161 ± 0.013), the average optical density of the ASM M3 receptor was significantly decreased in 6-month post-BT, 1-month post-CBA, and 6-month post-CBA groups (0.070 ± 0.022, 0.072 ± 0.012, 0.074 ± 0.008, respectively; all P < 0.001). There was no significant difference in the average optical density of ASM M3 receptor between the BT and CBA therapy groups after six months (P = 0.613).Conclusions:CBA therapy effectively ablates the ASM, and its ablation effect is equivalent to that of BT with a shorter onset time. A neural mechanism is involved in both BT and CBA therapy.

简介：Artesunaeisaderivativeofqinghaosu,withasesquiterpenestructure.Thespecificactionandtheclinicalusesofartesunateareonthepreliminarystage,ontheonehand,artesunatehasspecificactionofbothantiinflammationandantivirus,andalsohasprotectiveeffectonthepulmonaryalveolarmacrophages,whuichmaybeadvantageoustothetreatmentoftheairwaynon-specificinflammationofasthma,.Ontheotherhand,qinghaousuhastheactivitiestorelaxvascularsmoothmuscleandtocausehypotension.Theexpectorantaction,theantitussiveactionandtheantuiasthmaticactionofqinghaosuwerepreported.Artesunatemayalsohaveantiasthmaticactivity,becausetheantimalarialpotencyofartesunateisstrongerthanthatofqinghaosu,andArtesunatecanblockCa^2+influxbyinhibitingcalcium-dependentchloridecurrent.Themainaimsofthispaperaretoinvestigatethesite,themode,andthemechanismofartesunateactiononisolatedtrachealsmoothmusclefromtheguinea-pig.

简介：ＥｆｅｃｔｓｏｆＡｒｔｅｓｕｎａｔｅｏｎＴｒａｃｈｅａｌＳｍｏｏｔｈＭｕｓｃｌｅｆｒｏｍｔｈｅＧｕｉｎｅａｐｉｇＰｏｓｔｇｒａｄｕａｔｅ∶ＭａｏＳｈｅｎｇＹａｎｇＳｕｐｅｒｖｉｓｏｒ∶ＡｓｏｃｉａｔｅＰｒｏｆ．ＪｉａｎＣｈｕＸｉａｏＤｅｐａ...

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简介：BackgroundAcutecoronarysyndrome(ACS)isaleadingcauseofmortalityandmorbidityworldwide,whichcomprisesunstableangina(UA)andacutemyocardialinfarction(AMI),andtheinvestigationofbiologicalmarkerstoassessthosemostatriskofrecurrentcardiovasculareventsisnecessary.MethodsSixty-sixhealthycontrolpeopleand67casesofUApatientswereenrolledinGuangdonggeneralhospital.Enzymelinkedimmunosorbentassay(ELISA)wasusedtodetectthelevelofplasmaACTG2.Thereceiveroperatingcharacteristiccurve(ROC)wasusedtoanalyzethepredictionvalueofACTG2forUA.AccordingtotheaveragelevelofplasmaACTG2inUApatients,UApatientsweredividedintothelowACTG2group(

简介：ObjectivesToevaluatetheimpactofstentimplantationonproliferationandapop-tosisininjuredmediavascularsmoothmusclecells(VSMC)andtoexplorethemechanismofrestenosisafterstentimplantation.MethodsFiftymaleNewZealandrabbitswererandomizedintotwogroups,includingballoongroupandstentgroup.Controlgroupwassetup.Thesampleswereharvestedon3,7,14,28,56daysafteroperationandthefollowinginvestigationwascarriedout:(1)Assessingtheexpressionofproliferatingcellnuclearantigen(PCNA)ofmediaVSMCbythemethodofimmunohistochemistry;(2)AnalyzingapoptosisofmediaVSMCbyDNAagarosegelelectrophoresisandTUNELtechnique.ResultsTheexpressionofPCNAandapoptosisinstentandballoongroupsweremarkedlyincreasedcomparedwithcontrolgroups.(1)StentgroupinducedsignificantincreasedexpressionofPCNAinthemediaVSMCcomparedwithballoongroupon3to28days.Onday7,thepositiveratesofPCNAwere24.36±0.55%vs18.74±1.09%

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简介：【摘要】：目的：研究smooth饵激光联合盆底治疗仪治疗压力性尿失禁的疗效。方法：在本院接受治疗的压力性尿失禁患者中择取45例作为观察对象，样本抽选时间为2020年12月至2022年2月期间，上述患者经单双号随机分组法被分为两组，对照组22例患者给予盆底治疗仪治疗，观察组23例患者接受smooth饵激光联合盆底治疗仪治疗。将两组患者的尿失禁问卷简表（ICIQ-SF）评分、盆底肌力、漏尿量改善情况进行对比。结果：观察组患者治疗后的盆底肌力明显优于对照组，P＜0.05；治疗后，观察组患者的ICIQ-SF评分明显低于对照组，漏尿量则明显较对照组少，P＜0.05。结论：smooth饵激光联合盆底治疗仪应用于压力性尿失禁患者临床治疗中的效果显著，具有广泛推广应用的价值。

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简介：ObjectivesToinvestigatetheeffectsoftestosteroneenanthate(TE)onserumlipidsandlipoproteinsmetabolismandtheexpressionofandrogenreceptor(AR),estrogenreceptorbeta(ER-β)andplateletderivedgrowthfactorbeta(PDGFR-β)inaorticvascularsmoothmuscletissues(VSMTs).MethodsFortyagedmaleratswererandomlydividedinto4groups,groupA(placebogroup),groupB(2.5mg/kgintramuscularinjectionofTEonceaweek),groupC(5.0mg/kgintramuscularinjectionofTEonceaweek),groupD(10.0mg,/kgintramuscularinjectionofTEonceaweek).Allanimalswerefedfreelyduring16-weektreatmentperiods.TheexpressionofAR,ER-βandPDGFR-βwerestudiedbyWesternbolt.ResultsAverageserumLDL-CwasloweringroupDthanthatingroupA(p＜0.01).Comparedwiththeothergroups,averageserumTCwasalsoloweringroupD(p＜0.05).ARexpressioninaorticvascularsmoothmuscletissuescouldberegulatedbyTE:99.50±21.74,125.38±28.68and101.98±15.42forTEconcentrationsat2.5mg/kg,5.0mg/kgand10.0mg/kg,respectively,theexpressionofER-βcouldberegulatedbyTE:92.34±18.68,47.72±18.12,82.13±23.50,andtheexpressionofPDGFR-βcouldberegulatedaswellbyTE:219.70±45.59,50.16±9.72,125.36±15.74(DataforAR,ER-βandPDGFR-βproteinbandintensitywereexpressedwithx±s,withcontrolgrouptakenas100).ConclusionsThisstudyindicatesthatandrogenshavesignificanteffectsonserumlipidsandlipoproteinmetabolism.Testosteroneenanthateat5.0mg/kgcanstimulatetheexpressionofAR,butinhibitetheexpressionofPDGFR.Testosteroneenanthateattheconcentrationsof5.0mg/kgand10.0mg/kgcaninhibitetheexpressionofER-β.

简介：论战的AT1受体自身抗体(AT1-AAs)与恶意的高血压或preeclampia在病人被描述了。而且，AT1-AAs高度与倔强的高血压被联系。脉管的光滑的肌肉房间(VSMC)的功能在血压的规定是重要的。我们调查了并且比较了血管收缩素II(AngII)和AT1-AAs的能力刺激细胞内部的钙动员和老鼠VSMC的细胞的增长。有倔强的高血压，有非倔强的高血压的24个病人和37normotensives的22个病人被招募。每个病人的浆液被ELISA为AT1-AAs的存在检测。从病人的sera的AngII和AT1-AAs被用来在vitro刺激老鼠VSMC。AT1-AAs分别地与倔强的高血压，非倔强的高血压和normotensives在病人的10/22，3/24和3/37被检测。AT1-AAs带了增加以一种剂量依赖者方式和VSMC的细胞的增长的细胞内部的钙动员就作为AngII。AT1-AAs引起的这两效果与losartan或相应于AT1受体的第二个细胞外的环的部分的肽被堵住。因为AT1-AAs就作为AngII在老鼠VSMC展出了药理学活动，他们可能在外部脉管的抵抗的举起并且在脉管的改变起一个作用。并且AT1-AAs被建议在抵抗包含到antihypertensive治疗。

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